Lovastatin (Synonyms: Mevinolin)

Name: Lovastatin
Brand: MedChemExpress
SKU: HY-N0504
Rating: 5.0 (35 reviews)

Cat. No.: HY-N0504 Purity: 99.75%: Data Sheet
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Lovastatin is a cell-permeable HMG-CoA reductase inhibitor used to lower cholesterol.

For research use only. We do not sell to patients.

CAS No. : 75330-75-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 35 publication(s) in Google Scholar

Other Forms of Lovastatin:

Lovastatin (Standard) In-stock
Epi lovastatin-d₃ Get quote
Lovastatin-d₉ (epimer mixture) Get quote
Lovastatin-d₃ Get quote

35 Publications Citing Use of MCE Lovastatin

RT-PCR

Bio/Physico-chemical Assay

Cell Imaging/Staining

In Vivo Efficacy Study

Cell Proliferation/Viability Assay

: Lovastatin purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Aug;45(8):1010-1037. [Abstract]; Denifanstat (10 mg/kg; i.g.; every day; from day7 to day20); Lovastatin(10 mg/kg; i.g.; every day; from day7 to day20); Simvastatin (50 mg/kg; i.g.; every day; from day7 to day20). Tumor growth curve of each group (4 mice/group). ns, not significant; * P < 0.05, **P < 0.01, ***P < 0.001.

: Lovastatin purchased from MedChemExpress. Usage Cited in: Toxicology. 2025 Oct 13:519:154309. [Abstract]; Renal tubular epithelial cells were treated with DMSO, 3 mM Pyruvic acid sodium, and 25 μM lovastatin (with or without 3 mM Pyruvic acid sodium) for 48 hours. ROS generation of the microarray epithelial cells was assessed by DCFH-DA staining.

: Lovastatin purchased from MedChemExpress. Usage Cited in: Toxicology. 2025 Oct 13:519:154309. [Abstract]; Renal tubular epithelial cells were treated with DMSO, 3 mM Pyruvic acid sodium, and 25 μM lovastatin (with or without 3 mM Pyruvic acid sodium) for 48 hours. Damage to renal tubular epithelial cells on the chip was measured using the LDH assay.

: Lovastatin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 26;15(1):6311. [Abstract]; Lovastatin (25 μM; 24 h). The effect of exogenous or endogenous cholesterol on cholesterol content in infected HEp-2 cells was determined using an Amplex^TM Red Cholesterol Assay Kit and immunofluorescence assay, respectively.

: Lovastatin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 May 26;14(1):3050. [Abstract]; Westernblot analysis of TRABID mRNA and protein levels in HeLa cells treated with 10 μM Lovastatin, 2 μg/ml Aphidicolin, 10 μM RO-3306, or 3 μM Nocodazole for 18 h.

: Lovastatin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 May 26;14(1):3050. [Abstract]; RT-qPCR analysis of TRABID mRNA and protein levels in HeLa cells treated with 10 μM Lovastatin, 2 μg/ml Aphidicolin, 10 μM RO-3306, or 3 μM Nocodazole for 18 h.

: Lovastatin purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2023 Jan:209:105497. [Abstract]; Pretreatment with AY9944 (15, 30 µM), U18666A (5, 10 µM), Lovastatin (5, 10 µM) and MβCD for 2 h can all significantly reduce the level of EV-A71 VP1 protein in HCT-8 cell.

: Lovastatin purchased from MedChemExpress. Usage Cited in: Cell Metab. 2020 Apr 7;31(4):741-754.e5. [Abstract]; Liver mRNA expression levels of 25 prioritized genes after one week of 0.02% Lovastatin (red) or control (black) treatment in male mice.

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Biological Activity
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Description

Lovastatin is a cell-permeable HMG-CoA reductase inhibitor used to lower cholesterol.

IC₅₀ & Target

HMG-CoA reductase^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	11.4 μM Compound: 1	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 23570542]
A549	IC₅₀	19.8 μM Compound: 1	Inhibition of HMG-CoA reductase in human A549 cells after 5 mins by spectrophotometric analysis Inhibition of HMG-CoA reductase in human A549 cells after 5 mins by spectrophotometric analysis	[PMID: 23570542]
HEK293	IC₅₀	28 μM Compound: Lovastatin lactone	TP_TRANSPORTER: inhibition of estradiol-17beta-glucuronide uptake(estradiol-17beta-glucuronide:0.02uM) in OATP1B1-expressing HEK293 cells TP_TRANSPORTER: inhibition of estradiol-17beta-glucuronide uptake(estradiol-17beta-glucuronide:0.02uM) in OATP1B1-expressing HEK293 cells	[PMID: 15616150]
HES	IC₅₀	13 nM Compound: mevinolin (lactone) 2	Concentration required to inhibit HMG-CoA reductase by 50% was determined in HES 9 cell line Concentration required to inhibit HMG-CoA reductase by 50% was determined in HES 9 cell line	[PMID: 1527791]
HT-29	IC₅₀	46.8 μM Compound: Lovastatin	Growth inhibition of human HT29 cells after 96 hrs by MTS assay Growth inhibition of human HT29 cells after 96 hrs by MTS assay	[PMID: 17472962]
HepG2	IC₅₀	0.029 μM Compound: 1	Tested for inhibition of cholesterol biosynthesis in HEP G2 cells Tested for inhibition of cholesterol biosynthesis in HEP G2 cells	[PMID: 7932551]
HepG2	IC₅₀	0.079 μM Compound: 2	Tested for ability to inhibit incorporation of [14C]acetate into cholesterol in cultured human hepatoma (HEP-G2) cells; 0.061-0.10 Tested for ability to inhibit incorporation of [14C]acetate into cholesterol in cultured human hepatoma (HEP-G2) cells; 0.061-0.10	[PMID: 8246237]
HepG2	IC₅₀	37 nM Compound: mevinolin (lactone) 2	Concentration required to inhibit HMG-CoA reductase by 50% was determined in Hep G2 cell line Concentration required to inhibit HMG-CoA reductase by 50% was determined in Hep G2 cell line	[PMID: 1527791]
HepG2	IC₅₀	39 nM Compound: Mevinolin	Compound was evaluated for inhibitory activity against HMG-CoA reductase in HepG2 cells Compound was evaluated for inhibitory activity against HMG-CoA reductase in HepG2 cells	10.1016/S0960-894X(00)80623-X
HepG2	IC₅₀	50 nM Compound: 1b (Mevinolin)	Inhibition of cellular HMG-CoA reductase in cultures of human HEP G2 cells, determined by decreased incorporation of sodium [14C]acetate into cholesterol. Inhibition of cellular HMG-CoA reductase in cultures of human HEP G2 cells, determined by decreased incorporation of sodium [14C]acetate into cholesterol.	[PMID: 2296036]
HepG2	IC₅₀	50 nM Compound: mevinolin	Inhibition of the incorporation of sodium [14C]acetate into cholesterol in HEP G2 cells. Inhibition of the incorporation of sodium [14C]acetate into cholesterol in HEP G2 cells.	[PMID: 1656041]
HepG2	IC₅₀	8.3 μM Compound: Lovastatin	Lipid lowering activity in human HepG2 cells assessed as decrease in oleic acid elicited lipid accumulation after 6 hrs by oil-red O staining method Lipid lowering activity in human HepG2 cells assessed as decrease in oleic acid elicited lipid accumulation after 6 hrs by oil-red O staining method	[PMID: 26169125]
Hepatocyte	IC₅₀	32 nM Compound: Mevinolin	Compound was evaluated for inhibitory activity against HMG-CoA reductase from rat hepatocyte Compound was evaluated for inhibitory activity against HMG-CoA reductase from rat hepatocyte	10.1016/S0960-894X(00)80623-X
Hs68	IC₅₀	23.2 μM Compound: 1	Cytotoxicity against human HS68 cells after 72 hrs by MTT assay Cytotoxicity against human HS68 cells after 72 hrs by MTT assay	[PMID: 23570542]
KB	IC₅₀	15.6 μM Compound: 2	Cytotoxicity against human KB cells by resazurin microplate assay Cytotoxicity against human KB cells by resazurin microplate assay	[PMID: 27228159]
LS180	IC₅₀	25.3 μM Compound: Lovastatin	Growth inhibition of human LS180 cells after 96 hrs by MTS assay Growth inhibition of human LS180 cells after 96 hrs by MTS assay	[PMID: 17472962]
MCF7	IC₅₀	> 40 μM Compound: 1	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 24 to 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 24 to 48 hrs by MTT assay	[PMID: 27756564]
MDA-MB-231	IC₅₀	> 40 μM Compound: 1	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 24 to 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 24 to 48 hrs by MTT assay	[PMID: 27756564]
MDCK	IC₅₀	10 μM Compound: Lovastatin lactone	TP_TRANSPORTER: inhibition of calcein-AM efflux in MDR1-expressing MDCK cells TP_TRANSPORTER: inhibition of calcein-AM efflux in MDR1-expressing MDCK cells	[PMID: 15616150]
MEF	IC₅₀	35 μM Compound: 1	Cytotoxicity against mouse MEF cells after 72 hrs by MTT assay Cytotoxicity against mouse MEF cells after 72 hrs by MTT assay	[PMID: 23570542]
NIH-3T3-G185	IC₅₀	114.4 μM Compound: Lovastatin	TP_TRANSPORTER: inhibition of Rhodamine 123 efflux in NIH-3T3-G185 cells TP_TRANSPORTER: inhibition of Rhodamine 123 efflux in NIH-3T3-G185 cells	[PMID: 11716514]
NIH-3T3-G185	IC₅₀	32.7 μM Compound: Lovastatin	TP_TRANSPORTER: inhibition of LDS-751 efflux in NIH-3T3-G185 cells TP_TRANSPORTER: inhibition of LDS-751 efflux in NIH-3T3-G185 cells	[PMID: 11716514]
PC-3	IC₅₀	5.4 μM Compound: 1	Cytotoxicity against human PC3 cells assessed as growth inhibition after 48 hrs by SRB assay Cytotoxicity against human PC3 cells assessed as growth inhibition after 48 hrs by SRB assay	[PMID: 27756564]
SW480	IC₅₀	7.1 μM Compound: Lovastatin	Growth inhibition of human SW480 cells after 96 hrs by MTS assay Growth inhibition of human SW480 cells after 96 hrs by MTS assay	[PMID: 17472962]
T47D	IC₅₀	> 40 μM Compound: 1	Cytotoxicity against human T47D cells assessed as growth inhibition after 24 to 48 hrs by MTT assay Cytotoxicity against human T47D cells assessed as growth inhibition after 24 to 48 hrs by MTT assay	[PMID: 27756564]
THP-1	IC₅₀	8 μg/mL Compound: Lovastatin	Inhibition of sICAM1/LFA1 interaction-mediated human THP1 cell adhesion after 1 hr by ELISA Inhibition of sICAM1/LFA1 interaction-mediated human THP1 cell adhesion after 1 hr by ELISA	[PMID: 18672369]
Vero	IC₅₀	2.2 μM Compound: 2	Cytotoxicity against African green monkey Vero cells Cytotoxicity against African green monkey Vero cells	[PMID: 27228159]

In Vitro

When Lovastatin is used in cell function experiments, it is usually necessary to convert it from the inactive "lactone ring" form to the active "open-ring hydroxy acid" form^[6]^[7].
1. Dissolve 10 mg of Lovastatin in 0.2 mL of warm (55°C) anhydrous ethanol. Ultrasonic-assisted dissolution of soluble clear.
2. Add 0.1 mL of 0.6 M NaOH and 2 mL of aqueous solution and incubate at room temperature for 30 minutes.
3. Adjust the pH to 8.0 with HCl.
4. Finally, bring the volume to 2.47 mL for a final concentration of 10 mM.

Lovastatin (10 μM; 72 hours) efficiently reduces viability of HepG2 cells^[2].
Lovastatin (10 μM; 48 hours) induces apoptosis in HepG2 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	HepG2 cells
Concentration:	10 μM
Incubation Time:	72 hours
Result:	Efficiently reduced viability of HepG2 cells.

In Vivo

Lovastatin is an inactive lactone that is hydrolyzed in the liver to an active f3-hydroxyacid form. This principal metabolite is the inhibitor of the enzyme HMG-CoA reductase. The K_i is 1 nM. Lovastatin and its β-hydroxyacid metabolite are highly bound to human plasma proteins. Lovastatin crosses the blood-brain and placental barriers^[3]. Lovastatin produces a profound reduction of apolipoprotein-B-containing lipoproteins, especially LDL cholesterol and, to a lesser extent, plasma triglycerides, and a small increase in HDL cholesterol^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

404.54

Formula

C₂₄H₃₆O₅

CAS No.

75330-75-5

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C([C@@H](C)CC)O[C@@H]1[C@@]([C@H]2CC[C@@H](OC3=O)C[C@H](C3)O)([H])C(C=C[C@@H]2C)=C[C@H](C)C1

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

aspergillus terreus

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (247.19 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 50 mg/mL (123.60 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4719 mL	12.3597 mL	24.7194 mL
5 mM	0.4944 mL	2.4719 mL	4.9439 mL
10 mM	0.2472 mL	1.2360 mL	2.4719 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.75%

Select Batch:

Data Sheet (283 KB) SDS (419 KB)

COA (255 KB) HNMR (288 KB) RP-HPLC (247 KB) LCMS (102 KB)

Handling Instructions (2659 KB)

References

[1]. Alberts AW, et al. Discovery, biochemistry and biology of lovastatin. Am J Cardiol. 1988 Nov 11;62(15):10J-15J. [Content Brief]

[2]. Kah J, et al. Selective induction of apoptosis by HMG-CoA reductase inhibitors in hepatoma cells and dependence on p53 expression. Oncol Rep. 2012 Sep;28(3):1077-83. [Content Brief]

[3]. Frishman WH, et al. Lovastatin: an HMG-CoA reductase inhibitor for lowering cholesterol. Med Clin North Am. 1989 Mar;73(2):437-48. [Content Brief]

[4]. Tobert JA, et al. Lovastatin and beyond: the history of the HMG-CoA reductase inhibitors. Nat Rev Drug Discov. 2003 Jul;2(7):517-26. [Content Brief]

[5]. Ifergan I, et al. Statins reduce human blood-brain barrier permeability and restrict leukocyte migration: relevance to multiple sclerosis. Ann Neurol. 2006 Jul;60(1):45-55. [Content Brief]

[6]. Chu W, et al. Lovastatin exerts protective effects on endothelial cells via upregulation of PTK2B. Exp Ther Med. 2016 Sep;12(3):1741-1749. [Content Brief]

[7]. Régrigny O, et al. Effect of lovastatin on cerebral circulation in spontaneously hypertensive rats. Hypertension. 2000 May;35(5):1105-10. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	2.4719 mL	12.3597 mL	24.7194 mL	61.7986 mL
	5 mM	0.4944 mL	2.4719 mL	4.9439 mL	12.3597 mL
	10 mM	0.2472 mL	1.2360 mL	2.4719 mL	6.1799 mL
	15 mM	0.1648 mL	0.8240 mL	1.6480 mL	4.1199 mL
	20 mM	0.1236 mL	0.6180 mL	1.2360 mL	3.0899 mL
	25 mM	0.0989 mL	0.4944 mL	0.9888 mL	2.4719 mL
	30 mM	0.0824 mL	0.4120 mL	0.8240 mL	2.0600 mL
	40 mM	0.0618 mL	0.3090 mL	0.6180 mL	1.5450 mL
	50 mM	0.0494 mL	0.2472 mL	0.4944 mL	1.2360 mL
	60 mM	0.0412 mL	0.2060 mL	0.4120 mL	1.0300 mL
	80 mM	0.0309 mL	0.1545 mL	0.3090 mL	0.7725 mL
	100 mM	0.0247 mL	0.1236 mL	0.2472 mL	0.6180 mL

Lovastatin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lovastatin75330-75-5MevinolinHMG-CoA Reductase (HMGCR)AutophagyFerroptosisInhibitorinhibitorinhibit

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Lovastatin (Synonyms: Mevinolin)

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Other Forms of Lovastatin:

Lovastatin Related Antibodies

35 Publications Citing Use of MCE Lovastatin

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References

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Lovastatin Related Antibodies

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Complete Stock Solution Preparation Table

Lovastatin Related Classifications

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