NAD+ (Synonyms: β-DPN; β-NAD; β-Nicotinamide Adenine Dinucleotide)

Name: NAD+
Brand: MedChemExpress
SKU: HY-B0445
Rating: 5.0 (45 reviews)

Cat. No.: HY-B0445 Purity: 99.96%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

NAD+ is a coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 53-84-9

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	해외재고보유	Estimated Time of Arrival: December 31
Solid
500 mg	해외재고보유	Estimated Time of Arrival: December 31
1 g	해외재고보유	Estimated Time of Arrival: December 31
5 g	해외재고보유	Estimated Time of Arrival: December 31
10 g	해외재고보유	Estimated Time of Arrival: December 31
25 g	해외재고보유	Estimated Time of Arrival: December 31
50 g	해외재고보유	Estimated Time of Arrival: December 31
> 100 g	견적 받기

or 대량구매 문의

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 45 publication(s) in Google Scholar

Other Forms of NAD+:

α-NAD In-stock
NAD⁺-¹³C₅ ammonium In-stock
NAD+-d₄ In-stock
NAD sodium In-stock
NAD+ lithium In-stock
NAD+ (GMP Like) In-stock

45 Publications Citing Use of MCE NAD+

Cell Proliferation/Viability Assay

RT-PCR

: NAD+ purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Feb:69:103030. [Abstract]; LDH release assay showed the glioma cell death caused by SRT2183 (40 μmol/L) at 24 h was attenuated when the cells were pretreated 1 h with EX527 (200 μmol/L) or supplemented with exogenous NAD⁺ (2 mmol/L).

: NAD+ purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Feb:69:103030. [Abstract]; The declines of cysteine induced by SRT2183 (40 μmol/L) at 24 h were both suppressed by pretreating the cells 1 h with EX527 or supplementing exogenous NAD⁺ (2 mmol/L).

: NAD+ purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Feb:69:103030. [Abstract]; Western blotting revealed RSL3-induced downregulation of SLC7A11 and GPX4 was enhanced by FK866, but attenuated by exogenous NAD⁺ (2 mmol/L) supplement.

: NAD+ purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Feb:69:103030. [Abstract]; Pretreatment with FK866 (500 μmol/L) or supplement of exogenous NAD⁺ at 2 mmol/L for 1 h prevented RSL3-induced upregulation of ATF3 in both cytoplasmic and nuclear fractions.

: NAD+ purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Feb:69:103030. [Abstract]; Supplement of exogenous NAD⁺ at 1 mmol/L could inhibit ATF3 upregulation in the cells treated with FK866 (500 μmol/L) for 24 h, and this inhibition became more apparent by increasing NAD⁺ dosage to 2 mmol/L.

: NAD+ purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Oct;44(10):2125-2138. [Abstract]; Western blotting proved supplement of exterior NAD⁺ (0.5-2 mmol/L, 1 h) dosage-dependently alleviated DPT-induced downregulation of phospho-AKT and upregulation of NOX2, NAT10, phospho-CREB, PARP1, and PAR.

: NAD+ purchased from MedChemExpress. Usage Cited in: Vascul Pharmacol. 2022 Dec:147:107126. [Abstract]; NAD+ significantly improves the expression of CD31 in the MI mice and DM + MI mice.

: NAD+ purchased from MedChemExpress. Usage Cited in: Vascul Pharmacol. 2022 Dec:147:107126. [Abstract]; NAD+ significantly improves the expression of VEGF in the MI mice and DM + MI mice.

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

NAD+ is a coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage.

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

NAD+ is a coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. NAD+ is the oxidized form of NADH^[1]. NAD+ is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (Nadide) ^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Oral NAD+ supplementation has been used to combat simple fatigue as well as such mysterious and energy-sapping disorders as chronic fatigue syndrome and fibromyalgia^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

분자량

663.43

화학식

C₂₁H₂₇N₇O₁₄P₂

CAS No.

53-84-9

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1[C@H](O)[C@@H](COP(OP(OC[C@@H](O[C@H]([C@@H]2O)N3C=NC4=C3N=CN=C4N)[C@H]2O)(O)=O)([O-])=O)O[C@H]1[N+]5=CC(C(N)=O)=CC=C5

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

용액&용해도

In Vitro:

H₂O : 50 mg/mL (75.37 mM; Need ultrasonic)

H₂O : 35 mg/mL (52.76 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5073 mL	7.5366 mL	15.0732 mL
5 mM	0.3015 mL	1.5073 mL	3.0146 mL
10 mM	0.1507 mL	0.7537 mL	1.5073 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (150.73 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

순도&문서

Purity: 99.96%

Select Batch:

Data Sheet (273 KB) SDS (419 KB)

COA (249 KB) HNMR (292 KB) CNMR (291 KB) RP-HPLC (238 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Viollet, B., et al., Cellular and molecular mechanisms of metformin: an overview. Clin Sci (Lond), 2012. 122(6): p. 253-70. [Content Brief]

[2]. Brandt, U., Energy converting NADH:quinone oxidoreductase (complex I). Annu Rev Biochem, 2006. 75: p. 69-92. [Content Brief]

[3]. Kussmaul, L. and J. Hirst, The mechanism of superoxide production by NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria. Proc Natl Acad Sci U S A, 2006. 103(20): p. 7607-12. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / H₂O	1 mM	1.5073 mL	7.5366 mL	15.0732 mL	37.6830 mL
	5 mM	0.3015 mL	1.5073 mL	3.0146 mL	7.5366 mL
	10 mM	0.1507 mL	0.7537 mL	1.5073 mL	3.7683 mL
	15 mM	0.1005 mL	0.5024 mL	1.0049 mL	2.5122 mL
	20 mM	0.0754 mL	0.3768 mL	0.7537 mL	1.8841 mL
	25 mM	0.0603 mL	0.3015 mL	0.6029 mL	1.5073 mL
	30 mM	0.0502 mL	0.2512 mL	0.5024 mL	1.2561 mL
	40 mM	0.0377 mL	0.1884 mL	0.3768 mL	0.9421 mL
	50 mM	0.0301 mL	0.1507 mL	0.3015 mL	0.7537 mL
H₂O	60 mM	0.0251 mL	0.1256 mL	0.2512 mL	0.6280 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

NAD+ Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NAD+53-84-9β-DPN β-NAD β-Nicotinamide Adenine DinucleotideEndogenous MetaboliteInhibitorinhibitorinhibit

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NAD+ (Synonyms: β-DPN; β-NAD; β-Nicotinamide Adenine Dinucleotide)

고객리뷰

Other Forms of NAD+:

NAD+ Related Antibodies

45 Publications Citing Use of MCE NAD+

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

순도&문서

References

고객리뷰

NAD+ Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

NAD+ Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report