Napabucasin (Synonyms: ナパブカシン; BBI608)

Name: Napabucasin
Brand: MedChemExpress
SKU: HY-13919
Rating: 5.0 (26 reviews)

製品番号: HY-13919 純度: 99.94%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

Napabucasin (BBI608) is a STAT3 inhibitor which blocks stem cell activity in cancer cells.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 83280-65-3

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 84	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 84	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$77	在庫あり	Estimated Time of Arrival: December 31
10 mg	$110	在庫あり	Estimated Time of Arrival: December 31
25 mg	$190	在庫あり	Estimated Time of Arrival: December 31
50 mg	$275	在庫あり	Estimated Time of Arrival: December 31
100 mg	$495	在庫あり	Estimated Time of Arrival: December 31
200 mg	$715	在庫あり	Estimated Time of Arrival: December 31
500 mg	$1375	在庫あり	Estimated Time of Arrival: December 31
1 g	$1815	在庫あり	Estimated Time of Arrival: December 31
5 g		お問い合わせ
10 g		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 26 publication(s) in Google Scholar

顧客検証

Flow Cytometry

Cell Migration/Invasion Assay

2D/3D Cell Culture and Differentiation

IHC

Cell Proliferation/Viability Assay

: Napabucasin purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2019 Jul 5;38(1):289. [Abstract]; Proliferation of glioma cells treated with Napabucasin (0-10 μM, 24-48 h) and detected by CCK-8 assays. Half maximal inhibitory concentrations (IC₅₀) of Napabucasin in U87MG and LN229 cells were 6.4 and 5.6 μM respectively.

: Napabucasin purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2019 Jul 5;38(1):289. [Abstract]; After treatment with the indicated doses of Napabucasin (0, 1, 5, and 10 μM) for 48 h, protein levels of STAT3 in U87MG and LN229 cells were evaluated by western blotting.

: Napabucasin purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2019 Jul 5;38(1):289. [Abstract]; IF was used to estimate the expression of STAT3 in U87MG and LN229 cells after treatment with Napabucasin (5 μM, 48 h) (scale bar: 50 μm).

: Napabucasin purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2019 Jul 5;38(1):289. [Abstract]; EdU incorporation assays showed that exposure to 5 μM Napabucasin for 48 h impaired DNA synthesis in U87MG and LN229 cells.

: Napabucasin purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2019 Jul 5;38(1):289. [Abstract]; Napabucasin (5 μM, 48 h) arrests the GBM cell cycle and induces apoptosis. Cell cycle distribution of glioma cells analyzed by flow cytometry.

: Napabucasin purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2019 Jul 5;38(1):289. [Abstract]; Napabucasin represses the migration and invasion of GBM cells. Representative images of the wound healing assay showing that Napabucasin treatment (1 μM, 24 h) inhibited glioma cell migration.

: Napabucasin purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2019 Jul 5;38(1):289. [Abstract]; Napabucasin impairs the stemness of GBM cells. GSCs from U87MG and LN229 cells were grown in serum-free DMEM/F12 medium with or without Napabucasin (5 μM) for 10 days. Sphere morphology was photographed under a light microscope and spheroids were counted.

: Napabucasin purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2019 Jul 5;38(1):289. [Abstract]; Napabucasin (40 mg/kg, intraperitoneal injection, every other day ) suppresses tumor growth in an orthotopic glioma model. Immunohistochemistry was used to assess the expression of STAT3, MMP2, Ki-67, cyclin D1, β-catenin, and c-Myc in orthotopic xenografted tumors after vehicle or Napabucasin treatments.

: Napabucasin purchased from MedChemExpress. Usage Cited in: Cancer Med. 2016 Jun;5(6):1251-8. [Abstract]; Representative images of colony formation assay in PCa cell lines (PC-3 and 22RV1) and histological analysis of the colony number show that the colony formation of PCa cell lines are dramatically decreased after treated with 1 μM Napabucasin for 24 h.

STAT アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

STAT3 STAT5 STAT6 STAT1

生物活性
プロトコル
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

Napabucasin (BBI608) is a STAT3 inhibitor which blocks stem cell activity in cancer cells.

IC₅₀ & Target^[1]

STAT3

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.12 μM Compound: 2	Antiproliferative activity against human A549 cells assessed as inhibition of cell growth measured after 72 hrs by WST-8 method Antiproliferative activity against human A549 cells assessed as inhibition of cell growth measured after 72 hrs by WST-8 method	[PMID: 37343498]
A549	IC₅₀	0.12 μM Compound: 6; BBI608	Cytotoxicity against human A549 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay Cytotoxicity against human A549 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay	[PMID: 32044231]
A549	IC₅₀	0.74 μM Compound: Nap; BBI608	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 28558333]
A549	IC₅₀	6.69 μM Compound: BBI608	Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 48 hrs MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured after 48 hrs MTT assay	[PMID: 32631508]
AGS	IC₅₀	437 nM Compound: BBI608	Antiproliferative activity against human AGS cells assessed as reduction in cell viability incubated for 72 hrs by MTS assay Antiproliferative activity against human AGS cells assessed as reduction in cell viability incubated for 72 hrs by MTS assay	[PMID: 36103247]
BXPC-3	IC₅₀	1.3 μM Compound: NP	Antiproliferative activity against human BxPC-3 cells harboring ZFP91siRNA assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human BxPC-3 cells harboring ZFP91siRNA assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 33506674]
BXPC-3	IC₅₀	1.4 μM Compound: NP	Antiproliferative activity against human BxPC-3 cells assessed as reduction in cell viability incubated for 3 days by MTT assay Antiproliferative activity against human BxPC-3 cells assessed as reduction in cell viability incubated for 3 days by MTT assay	[PMID: 33506674]
BXPC-3	IC₅₀	1.8 μM Compound: NP	Antiproliferative activity against ZFP91 knockdown human BxPC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against ZFP91 knockdown human BxPC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 33506674]
BXPC-3	IC₅₀	1962 nM Compound: 1c; BBI608	Inhibition of ATP production in human BXPC-3 cells incubated for 20 to 24 hrs by ATP assay kit method Inhibition of ATP production in human BXPC-3 cells incubated for 20 to 24 hrs by ATP assay kit method	[PMID: 36282975]
BXPC-3	IC₅₀	5306 nM Compound: 1c; BBI608	Anti-proliferative activity against human BXPC-3 cells assessed as cell growth inhibition incubated for 72 hrs by MTS assay Anti-proliferative activity against human BXPC-3 cells assessed as cell growth inhibition incubated for 72 hrs by MTS assay	[PMID: 36282975]
CT26	IC₅₀	2.5 μM Compound: Napabucasin	Antiproliferative activity against mouse CT26 cells after 72 hrs by MTT assay Antiproliferative activity against mouse CT26 cells after 72 hrs by MTT assay	[PMID: 30472602]
Capan-2	IC₅₀	1586 nM Compound: 1c; BBI608	Anti-proliferative activity against human Capan-2 cells assessed as cell growth inhibition incubated for 72 hrs by MTS assay Anti-proliferative activity against human Capan-2 cells assessed as cell growth inhibition incubated for 72 hrs by MTS assay	[PMID: 36282975]
Col2	ED₅₀	0.4 μg/mL Compound: 5	Cytotoxicity against human Col2 cells Cytotoxicity against human Col2 cells	[PMID: 10785421]
DLD-1	IC₅₀	944 nM Compound: NAP	Antiproliferative activity against human DLD-1 cells harboring STAT3 over activation assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against human DLD-1 cells harboring STAT3 over activation assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 38704936]
DU-145	IC₅₀	23.04 nM Compound: 3	Anticancer activity against human DU145 cells after 72 hrs by XTT assay Anticancer activity against human DU145 cells after 72 hrs by XTT assay	[PMID: 18829316]
HCT-116	IC₅₀	0.3 μM Compound: 87; BBI608	Antiproliferative activity against human HCT-116 cells assessed as reduction in cell proliferation incubated for 72 hrs by CellTiter-Glo assay Antiproliferative activity against human HCT-116 cells assessed as reduction in cell proliferation incubated for 72 hrs by CellTiter-Glo assay	[PMID: 33445154]
HCT-116	IC₅₀	620.32 nM Compound: NAP	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 38704936]
HCT-15	IC₅₀	660.3 nM Compound: NAP	Antiproliferative activity against human HCT-15 cells harboring STAT3 over activation assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against human HCT-15 cells harboring STAT3 over activation assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 38704936]
HEK293	IC₅₀	10.4 μM Compound: NP	Antiproliferative activity against human HEK293 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human HEK293 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33506674]
HT-29	IC₅₀	0.14 μM Compound: BBI608	Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability measured after 48 hrs MTT assay Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability measured after 48 hrs MTT assay	[PMID: 32631508]
HT-29	IC₅₀	1.6 μM Compound: Napabucasin	Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 30472602]
HT-29	IC₅₀	1253.7 nM Compound: NAP	Antiproliferative activity against human HT-29 cells harboring STAT3 over activation assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against human HT-29 cells harboring STAT3 over activation assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 38704936]
HUVEC	IC₅₀	41.28 μM Compound: BBI608	Antiproliferative activity against human HUVEC cells assessed as inhibition of cell growth in measured after 72 hrs by MTT assay Antiproliferative activity against human HUVEC cells assessed as inhibition of cell growth in measured after 72 hrs by MTT assay	[PMID: 34435487]
HaCaT	IC₅₀	0.5 μM Compound: 4a	Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy	[PMID: 22845014]
HaCaT	IC₅₀	0.5 μM Compound: 6	Antiproliferative activity against human HaCaT cells Antiproliferative activity against human HaCaT cells	[PMID: 10479319]
HepG2	IC₅₀	0.66 μM Compound: Nap; BBI608	Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 28558333]
HepG2	IC₅₀	0.84 μM Compound: BBI608	Antiproliferative activity against human HepG2 cells overexpressed STAT3 assessed as reduction in cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human HepG2 cells overexpressed STAT3 assessed as reduction in cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 32603980]
HepG2	IC₅₀	10.2 μM Compound: BBI 608	Antiproliferative activity against human HepG2 cells after 48 hrs by CCK-8 assay Antiproliferative activity against human HepG2 cells after 48 hrs by CCK-8 assay	[PMID: 29604543]
HepG2	IC₅₀	6.6 μM Compound: Napabucasin	Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 30472602]
K562	IC₅₀	1 μM Compound: 6	Antiproliferative activity against human K562 cells measured after 48 hrs by MTT assay Antiproliferative activity against human K562 cells measured after 48 hrs by MTT assay	[PMID: 30003778]
KB	ED₅₀	0.2 μg/mL Compound: 5	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 10785421]
KB	ED₅₀	1 μg/mL Compound: 1	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 7153777]
KB	ED₅₀	1 μg/mL Compound: 19	Evaluated for cytotoxicity in the KB cell culture assay Evaluated for cytotoxicity in the KB cell culture assay	[PMID: 3669007]
KB	ED₅₀	4.2 μM Compound: 2	Effective cytotoxic dose against KB cells was evaluated Effective cytotoxic dose against KB cells was evaluated	[PMID: 9873618]
L02	IC₅₀	18.63 μM Compound: Napa; BBI-608	Antiproliferative activity against human L02 cells assessed as cell viability incubated for 48 hrs measured after additional incubation with MTT Antiproliferative activity against human L02 cells assessed as cell viability incubated for 48 hrs measured after additional incubation with MTT	[PMID: 39226127]
L02	IC₅₀	18.7 μM Compound: BBI 608	Antiproliferative activity against human L02 cells after 48 hrs by CCK-8 assay Antiproliferative activity against human L02 cells after 48 hrs by CCK-8 assay	[PMID: 29604543]
LNCaP	ED₅₀	0.2 μg/mL Compound: 5	Cytotoxicity against human LNCaP cells Cytotoxicity against human LNCaP cells	[PMID: 10785421]
M109	ED₅₀	0.9 μg/mL Compound: 5	Cytotoxicity against human M109 cells Cytotoxicity against human M109 cells	[PMID: 10785421]
MCF-10A	IC₅₀	0.54 μM Compound: BBI608	Cytotoxicity against human MCF10A cells assessed as reduction in cell viability measured after 48 hrs MTT assay Cytotoxicity against human MCF10A cells assessed as reduction in cell viability measured after 48 hrs MTT assay	[PMID: 32631508]
MCF-10A	IC₅₀	7.94 μM Compound: Napa; BBI-608	Antiproliferative activity against human MCF-10A cells assessed as cell viability incubated for 48 hrs measured after additional incubation with MTT Antiproliferative activity against human MCF-10A cells assessed as cell viability incubated for 48 hrs measured after additional incubation with MTT	[PMID: 39226127]
MCF7	EC₅₀	1.49 μM Compound: BBI-608	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by CyQuant assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by CyQuant assay	[PMID: 33352047]
MCF7	IC₅₀	0.24 μM Compound: 6; BBI608	Cytotoxicity against human MCF7 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay Cytotoxicity against human MCF7 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay	[PMID: 32044231]
MCF7	IC₅₀	0.63 μM Compound: BBI608	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability measured after 48 hrs MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability measured after 48 hrs MTT assay	[PMID: 32631508]
MCF7	IC₅₀	11.42 μM Compound: BBI608	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay	[PMID: 34435487]
MCF7	IC₅₀	< 1 μM Compound: 6; BBI608	Inhibition of IL-6 induced STAT3 phosphorylation at Tyr705 residue in human MCF7 cells preincubated for 1 hr followed by IL-6 stimulation and measured after 1 hr by Western blot analysis Inhibition of IL-6 induced STAT3 phosphorylation at Tyr705 residue in human MCF7 cells preincubated for 1 hr followed by IL-6 stimulation and measured after 1 hr by Western blot analysis	[PMID: 32044231]
MDA-MB-231	EC₅₀	1.8 μM Compound: BBI-608	Antiproliferative activity against human MDA-MB-231 cells harbouring STAT3 assessed as reduction in cell viability at upto 10 uM incubated for 72 hrs by CyQuant assay Antiproliferative activity against human MDA-MB-231 cells harbouring STAT3 assessed as reduction in cell viability at upto 10 uM incubated for 72 hrs by CyQuant assay	[PMID: 33352047]
MDA-MB-231	IC₅₀	0.25 μM Compound: 2	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 72 hrs by WST-8 method Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 72 hrs by WST-8 method	[PMID: 37343498]
MDA-MB-231	IC₅₀	0.25 μM Compound: 6; BBI608	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell proliferation measured after 72 hrs by WST8 assay	[PMID: 32044231]
MDA-MB-231	IC₅₀	0.34 μM Compound: Nap; BBI608	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 28558333]
MDA-MB-231	IC₅₀	0.7 μM Compound: BBI608	Antiproliferative activity against human MDA-MB-231 cells overexpressing STAT3 assessed as reduction in cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human MDA-MB-231 cells overexpressing STAT3 assessed as reduction in cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 32603980]
MDA-MB-231	IC₅₀	1.29 μM Compound: BBI608	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability measured after 48 hrs MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability measured after 48 hrs MTT assay	[PMID: 32631508]
MDA-MB-231	IC₅₀	10.97 μM Compound: BBI608	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay	[PMID: 34435487]
MDA-MB-231	IC₅₀	2.1 μM Compound: 6	Antiproliferative activity against human MDA-MB-231 cells measured after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells measured after 48 hrs by MTT assay	[PMID: 30003778]
MDA-MB-231	IC₅₀	2.94 μM Compound: Napa; BBI-608	Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability incubated for 48 hrs measured after additional incubation with MTT Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability incubated for 48 hrs measured after additional incubation with MTT	[PMID: 39226127]
MDA-MB-231	IC₅₀	9.68 μM Compound: BBI608	Antiproliferative activity against human drug tolerant MDA-MB-231/lapatinib cells assessed as inhibition of cell growth in measured after 72 hrs by MTT assay Antiproliferative activity against human drug tolerant MDA-MB-231/lapatinib cells assessed as inhibition of cell growth in measured after 72 hrs by MTT assay	[PMID: 34435487]
MDA-MB-468	IC₅₀	1.14 μM Compound: 1; BBI608	Antiproliferative activity against human MDA-MB-468 cells assessed as reduction in cell growth measured after 48 hrs by CCK8 assay Antiproliferative activity against human MDA-MB-468 cells assessed as reduction in cell growth measured after 48 hrs by CCK8 assay	[PMID: 33126089]
MDA-MB-468	IC₅₀	1.14 μM Compound: BBI608	Antiproliferative activity against human MDA-MB-468 cells overexpressing STAT3 assessed as reduction in cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human MDA-MB-468 cells overexpressing STAT3 assessed as reduction in cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 32603980]
MDA-MB-468	IC₅₀	5.22 μM Compound: Napa; BBI-608	Antiproliferative activity against human MDA-MB-468 cells assessed as cell viability incubated for 48 hrs measured after additional incubation with MTT Antiproliferative activity against human MDA-MB-468 cells assessed as cell viability incubated for 48 hrs measured after additional incubation with MTT	[PMID: 39226127]
MGC-803	IC₅₀	871.8 nM Compound: BBI608	Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability incubated for 72 hrs by MTS assay Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability incubated for 72 hrs by MTS assay	[PMID: 36103247]
MIA PaCa-2	IC₅₀	1.2 μM Compound: NP	Antiproliferative activity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 3 days by MTT assay Antiproliferative activity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 3 days by MTT assay	[PMID: 33506674]
MKN-45	IC₅₀	3943 nM Compound: BBI608	Antiproliferative activity against human MKN-45 cells assessed as reduction in cell viability incubated for 72 hrs by MTS assay Antiproliferative activity against human MKN-45 cells assessed as reduction in cell viability incubated for 72 hrs by MTS assay	[PMID: 36103247]
PC-3	IC₅₀	5.25 μM Compound: Napabucasin	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 30472602]
SK-N-SH	ED₅₀	0.8 μg/mL Compound: 5	Cytotoxicity against human SK-N-SH cells Cytotoxicity against human SK-N-SH cells	[PMID: 10785421]
SW480	IC₅₀	762.7 nM Compound: NAP	Antiproliferative activity against human SW480 cells harboring STAT3 over activation assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against human SW480 cells harboring STAT3 over activation assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 38704936]
SW626	ED₅₀	0.2 μg/mL Compound: 5	Cytotoxicity against human SW626 cells Cytotoxicity against human SW626 cells	[PMID: 10785421]
U-251	IC₅₀	2.6 μM Compound: Napabucasin	Antiproliferative activity against human U251 cells after 72 hrs by MTT assay Antiproliferative activity against human U251 cells after 72 hrs by MTT assay	[PMID: 30472602]

体外実験

Napabucasin inhibits the expressions of stemness markers and kill stemness-high cancer cells isolated from several kinds of tumors except PCa. Napabucasin not only inhibits cell proliferation, cell motility, cell survival, colony formation ability, and tumorigenic potential of PCa cells, and increases cell apoptosis and sensitivity to docetaxel, but also effectively blocks sphere formation of PrCSCs and kill them as well as inhibits stemness gene expression. Napabucasin inhibits cell proliferation in PC-3 cells and 22RV1 cells at 48, 72, 96, and 120 h (P<0.05). Cell motility and colony formation ability are closely correlated with the process of tumor metastasis. Napabucasin significantly decreases colony formation and cell motility ability of PCa cell lines in vitro (P<0.05). The proliferation of PC-3 and 22RV1 cells treated with 1 μM Napabucasin are significantly decreased from day 2 to 5 compared with the control group (P<0.05)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

Napabucasin (40 mg/kg) or Docetaxel significantly reduces xenograft tumor growth and tumor volume (TV) compared with PBS (P<0.05). Notably, while no differences are observed between the Napabucasin and the docetaxel groups in PC-3 mouse xenograft models, the TV in Napabucasin group is even lower than docetaxel group in 22RV1 mouse xenograft models (P<0.05). Additionally, Napabucasin or docetaxel also significantly reduces tumor weight compared with PBS (P<0.05)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

臨床実験

分子量

240.21

分子式

C₁₄H₈O₄

CAS 番号

83280-65-3

Appearance

Solid

Color

Light yellow to green yellow

SMILES

O=C(C1=C2OC(C(C)=O)=C1)C3=C(C2=O)C=CC=C3

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

N-Methylpyrrolidone (NMP) : 16.67 mg/mL (69.40 mM; Need ultrasonic)

DMSO : 4.44 mg/mL (18.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Ethanol : < 1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1630 mL	20.8151 mL	41.6302 mL
5 mM	0.8326 mL	4.1630 mL	8.3260 mL
10 mM	0.4163 mL	2.0815 mL	4.1630 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 5% NMP 40% PEG300 5% Tween-80 50% Saline
Solubility: 0.83 mg/mL (3.46 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 0.5 mg/mL (2.08 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 0.5 mg/mL (2.08 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 4

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.5 mg/mL (2.08 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.94%

Select Batch:

データシート (280 KB) SDS (393 KB)

COA (251 KB) HNMR (236 KB) LCMS (106 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Zhang Y, et al. Suppression of prostate cancer progression by cancer cell stemness inhibitor napabucasin. Cancer Med. 2016 Jun;5(6):1251-8. [Content Brief]

細胞実験 ^[1]	The antiproliferative activity of Napabucasin against the PCa cell lines PC-3 and 22RV1 is examined. For cell proliferation assay, the PCa cell lines (22RV1 and PC-3) are seeded in 96-well plates at 2×10³ cells/well in a final volume of 100 μL and incubated overnight. The proliferation of PC-3 and 22RV1 cells treated with 1 μM Napabucasin. The viability of cells is determined with CellTiter 96 non-radioactive cell proliferation assay (MTS). For colony formation assay, cells are placed in a six-well plate and maintained in RPMI-1640 supplemented with 10% FBS for 2 weeks. The colonies are fixed with 4% paraformaldehyde, stained with 0.1% crystal violet and counted^[1]. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
動物実験 ^[1]	Mice^[1] A total of 1×10⁶ PC-3 cells or 8×10⁶ 22RV1 cells in 100 μL of PBS are injected subcutaneously into dorsal flanks of an immunodeficient nude mouse. The animals are treated i.p. with Napabucasin (40 mg/kg), Docetaxel (10 mg/kg), or PBS q3d once the tumors have reached 50 mm³. The tumor volume (TV) is calculated every 4 days according to the following standard formula: TV (mm³)=length×width²×0.5. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
参考文献	[1]. Zhang Y, et al. Suppression of prostate cancer progression by cancer cell stemness inhibitor napabucasin. Cancer Med. 2016 Jun;5(6):1251-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / N-Methylpyrrolidone (NMP)	1 mM	4.1630 mL	20.8151 mL	41.6302 mL	104.0756 mL
	5 mM	0.8326 mL	4.1630 mL	8.3260 mL	20.8151 mL
	10 mM	0.4163 mL	2.0815 mL	4.1630 mL	10.4076 mL
	15 mM	0.2775 mL	1.3877 mL	2.7753 mL	6.9384 mL
N-Methylpyrrolidone (NMP)	20 mM	0.2082 mL	1.0408 mL	2.0815 mL	5.2038 mL
	25 mM	0.1665 mL	0.8326 mL	1.6652 mL	4.1630 mL
	30 mM	0.1388 mL	0.6938 mL	1.3877 mL	3.4692 mL
	40 mM	0.1041 mL	0.5204 mL	1.0408 mL	2.6019 mL
	50 mM	0.0833 mL	0.4163 mL	0.8326 mL	2.0815 mL
	60 mM	0.0694 mL	0.3469 mL	0.6938 mL	1.7346 mL

Napabucasin Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Napabucasin83280-65-3BBI608BBI 608BBI-608STATInhibitorinhibitorinhibit

日本の代理先をお選びくださいナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
製品名			カスタマ需要量 *
お名前 *			タイトル
メールアドレス *			電話番号 *
デパートメント			組纖名 *
市区町村			都道府県
国或いは地域 *
必ず会社名を記載ください。個人への返信は行いません。
備考

製品名			Lot #
お名前 *			メールアドレス *
電話番号 *			部門 *
組纖名 *			国或いは地域 *
備考

Napabucasin (Synonyms: ナパブカシン; BBI608)

カスタマーレビュー

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

STAT アイソフォーム固有の製品をすべて表示:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Napabucasin Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

お名前
メール *

	Sorry, but the email address you supplied was invalid.

Napabucasin (Synonyms: ナパブカシン; BBI608)

カスタマーレビュー

Napabucasin 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

STAT アイソフォーム固有の製品をすべて表示:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

Napabucasin 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Napabucasin Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report