1. Cell Cycle/DNA Damage
    Cytoskeleton
    Anti-infection
  2. Microtubule/Tubulin
    Parasite
  3. Parbendazole

Parbendazole (Synonyms: SKF 29044)

Cat. No.: HY-115364 Purity: 99.01%
Handling Instructions

Parbendazole is a potent inhibitor of microtubule assembly, destabilizes tubulin, with an EC50 of 530 nM, and exhibits a broad-spectrum anthelmintic activity.

For research use only. We do not sell to patients.

Parbendazole Chemical Structure

Parbendazole Chemical Structure

CAS No. : 14255-87-9

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 55 In-stock
Estimated Time of Arrival: December 31
Solid
10 mg USD 50 In-stock
Estimated Time of Arrival: December 31
25 mg USD 80 In-stock
Estimated Time of Arrival: December 31
50 mg USD 120 In-stock
Estimated Time of Arrival: December 31
100 mg USD 190 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products
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Description

Parbendazole is a potent inhibitor of microtubule assembly, destabilizes tubulin, with an EC50 of 530 nM, and exhibits a broad-spectrum anthelmintic activity.

IC50 & Target

EC50: 530 nM (tubulin)[1]

In Vitro

Parbendazole is a tubulin destabilizer, with an EC50 of 530 nM, and can induce DNA damage[1]. Parbendazole (2-10 μM) inhibits the assembly of microtubules dose-dependently, with an IC50 of 3 μM. Parbendazole (2-20 μM)-treated cells show an complete absence of microtubules in Vero cells[2]. Parbendazole (up to 10 μM) inhibits the growth of CLd-AXE myxamoebae. Parbendazole (2-5 μM) potently inhibits tubulin purified from the wild-type myxamoebae[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

247.29

Formula

C₁₃H₁₇N₃O₂

CAS No.
SMILES

O=C(OC)NC1=NC2=CC=C(CCCC)C=C2N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 4 mg/mL (16.18 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0438 mL 20.2192 mL 40.4384 mL
5 mM 0.8088 mL 4.0438 mL 8.0877 mL
10 mM 0.4044 mL 2.0219 mL 4.0438 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 0.4 mg/mL (1.62 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 0.4 mg/mL (1.62 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 0.4 mg/mL (1.62 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Kinase Assay
[2]

Pure tubulin is obtained from sheep brain by 2 cycles of assembly and disassembly in vitro. Immediately prior to use the protein is centrifuged at 130000 g for 30 min to remove any aggregates. It is used at a protein concentration of 0-2 mg/mL in 0.025 M Pipes buffer, 0-5 mM EGTA, 0-25 mM Mg2SOsup>4, 0.1 mM GTP. Drug binding is determined by equilibrium dialysis using concentrations of parbendazole between 0.1 μM and 4 μM, and 2% (v/v) DMF (dimethyl formamide) as a carrier. Equilibrium is achieved by constant stirring for 2 h at 26°C, bovine serum albumin being used as a standard. 200 μL aliquots are counted in PCS in a 25-200B liquid scintillation counter[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay
[2]

Vero cells, an established cell line derived from monkey kidney are seeded in DMEM supplemented with 10% (v/v) foetal calf serum onto glass coverslips in multiwell dishes. They are allowed to settle, and spread for 2-5 h in a humid atmosphere at 37°C. After this time the medium is changed to DMEM containing 2, 10 or 20 μM parbendazole and 1% (v/v) DMSO controls contained 1 % (v/v) DMSO or had no additions[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.01%

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Keywords:

ParbendazoleSKF 29044SKF29044SKF-29044Microtubule/TubulinParasiteInhibitorinhibitorinhibit

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Parbendazole
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HY-115364
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