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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1435
    Oxonol VI
    		98.18%
    Oxonol VI is an optical indicator of membrane potential in lipid vesicles (excitation/emission wavelengths: 614/646 nm). Oxonol VI can be used to detect changes in membrane potential associated with (Na+ + K+)-ATPase activity in reconstituted vesicles.
    Oxonol VI
  • HY-123275A
    H-D-Phe-Pip-Arg-pNA hydrochloride
    		98.44%
    H-D-Phe-Pip-Arg-pNA (S-2238) hydrochloride, a chromogenic substrate, is patterned after the N-terminal portion of the A alpha chain of fibrinogen, which is the natural substrate of thrombin. H-D-Phe-Pip-Arg-pNA hydrochloride is specific for thrombin and is used to measure antithrombin-heparin cofactor (AT-III). The AT-III assay using H-D-Phe-Pip-Arg-pNA hydrochloride is sensitive, accurate, and easy to perform.
    H-D-Phe-Pip-Arg-pNA hydrochloride
  • HY-D1459
    BODIPY 665/676
    		99.96%
    BODIPY 665/676 is a lipophilic radical-sensitive fluorescent probe that can be used to study radical-driven lipid autoxidation (Ex/Em=665/676 nm).
    BODIPY 665/676
  • HY-D1296
    Green DND-26
    		99.77%
    Green DND-26 is a green fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 504/511 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and generally gather on spherical organelles. Green DND-26 is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes.
    Green DND-26
  • HY-W879007
    ICG-azide
    		99.66%
    ICG-azide, a near infrared (NIR) fluorescent dye, is a copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reagent. ICG-azide can be readily incorporated into dye-stabilized nanoemulsions and facilitate the attachment of targeting ligands via click-chemistry in a simple, scalable, and reproducible reaction.
    ICG-azide
  • HY-123630
    Allura Red AC
    		99.00%
    Allura Red AC is a food colorant, appearing as a deep red water-soluble powder or granules, used in various applications such as beverages, syrups, candies, and cereals. Allura Red AC can statically quench the intrinsic fluorescence of HSA. Additionally, Allura Red AC is a 5-hydroxytryptamine (5-HT) pathway-associated pro-inflammatory agent, capable of exacerbating experimental colitis. Allura Red AC holds potential for research in inflammatory bowel disease (IBD), intestinal barrier function, and food additive safety.
    Allura Red AC
  • HY-DY1031
    FM4-64 (solution)
    FM4-64 (solution) is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM4-64 is widely used in endocytic and exospic membrane structure markers.
    Solvent and concentration: DMSO: 5 mM
    FM4-64 (solution)
  • HY-D1450
    IR-1048
    IR-1048 is a nitroreductase (NTR)-responsive near-infrared fluorescence (NIR)/photoacoustic (PA) imaging probe and photothermal agent. IR-1048 is coupled with a nitroimidazole group to form IR-1048-MZ. IR-1048-MZ is catalytically reduced by NTR in a hypoxic environment, restoring strong near-infrared absorption and fluorescence emission (NIR II window), while activating the photothermal effect. IR-1048 relies on NTR-mediated electron transfer to relieve intramolecular fluorescence quenching, achieving specific imaging and photothermal ablation of tumor hypoxic areas. IR-1048 is mainly used for high-contrast NIR II/photoacoustic imaging and photothermal therapy research and tumor diagnosis of the tumor hypoxic microenvironment.
    IR-1048
  • HY-P1883A
    Bacterial Sortase Substrate III, Abz/DNP TFA
    		98.09%
    Bacterial Sortase Substrate III, Abz/DNP TFA is a fluorescent peptide substrate. Bacterial Sortase Substrate III, Abz/DNP TFA undergoes cleavage catalyzed by Staphylococcus aureus sortase A (SrtAΔN24) and Streptococcus pyogenes sortase A (SrtAΔN81), and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of the cell wall cross-bridge. Cleavage of this substrate can be detected at Ex/Em=320 nm/420 nm.
    Bacterial Sortase Substrate III, Abz/DNP TFA
  • HY-D0168
    Orcinol
    		99.97%
    Orcinol (3,5-Dihydroxytoluene) is an organic compound used in biological dyeing and proteomics research. Orcinol inhibits melanogenesis in B16F10 cells by upregulating the MAPK/ERK signaling pathway, and suppresses the expression of MITF, tyrosinase (TYR), TRP1, and DCT. Orcinol exhibits certain DPPH radical scavenging activity. In addition, Orcinol can alter nitrogen balance in animals. Orcinol holds promise for research in cancer and metabolic diseases.
    Orcinol
  • HY-D0203
    Basic green 4
    Basic green 4 (Malachite green) is a cationic dye that is widely used as a bactericide in aquaculture and is also commonly used in laboratories for the determination of phosphates. Basic green 4 exhibits high cytotoxicity and carcinogenicity to mammalian cells, specifically shown by its IC50 values of 2.03 µM for HEp-2 human laryngeal cells and 13.8 µM for Caco-2 human colorectal adenocarcinoma cells, and it can promote the development of liver tumors. The maximum absorbance wavelength is 621 nm.
    Basic green 4
  • HY-158082D
    TRITC-dextran, MW 150000
    TRITC-dextran, MW 150000 (Tetramethyl rhodamine isothiocyanate glucan, MW 150000) is a fluorescent dye, with the molecular weight of 150 kD. TRITC-dextran, MW 150000 exhibits an excitation wavelength of 555 nm. TRITC-dextran, MW 150000 is follicular and vessel penetrate, which is dependent on the molecular weight. TRITC-dextran, MW 150000 is utilized in drug delivery for the stability of TRITC over a wide pH range (i.e. pH 2–11) and resistance to photo-bleaching.
    TRITC-dextran, MW 150000
  • HY-D1161
    True Blue
    True Blue (NCI 240899) is a fluorescent dye, as neuronal retrograde tracer (excitation wavelength 395-425 nm, barrier filter 450 nm). True Blue can label neuron and has no effects on neuronal survival.
    True Blue
  • HY-130533
    ReAsH-EDT2
    ReAsH-EDT2 is a red fluorescent dye that marks proteins. ReAsH-EDT2 is a membrane-permeable biarsenical compound that binds covalently to tetracysteine sequences which allows the protein to be imaged. ReAsH-EDT2 can be used for protein localization and trafficking. (λex=530 nm, λem=592 nm).
    ReAsH-EDT2
  • HY-D0723
    5(6)-TAMRA SE
    5(6)-TAMRA SE is a fluorescent dye that emits red fluorescence. 5(6)-TAMRA SE binds to oligonucleotides and is used in DNA sequencing. 5(6)-TAMRA SE can be used in cancer research (Ex/Em = 565/580 nm).
    5(6)-TAMRA SE
  • HY-D0945
    Xylene Cyanol FF
    Xylene Cyanol FF is an acid triphenylmethane dye. Xylene Cyanol FF can be used for histochemical staining of hemoglobin peroxidase or as a tracking dye for DNA sequencing in electrophoresis. Xylene Cyanol FF will be catalyzed by Fe and Al to accelerate oxidation under the addition of double oxidant hydrogen peroxide and potassium periodate. Xylene Cyanol FF thus enables the spectrophotometric determination of Fe and Al in the solution to be tested.
    Xylene Cyanol FF
  • HY-P5354
    FRETS-VWF73
    FRETS-VWF73, a 73-amino-acid peptide, is a fluorogenic substrate for ADAMTS13 assay (Ex = 340 nm; Em = 450 nm). FRETS-VWF73 is a predictive tool for thrombotic thrombocytopenic purpura.
    FRETS-VWF73
  • HY-D0217
    Sulfobromophthalein disodium salt
    		99.77%
    Sulfobromophthalein (Bromosulfophthalein) disodium salt is an organic anion dye used in the study of a variety of membrane carriers expressed in animal tissues and involved in transport of agents and metabolites.
    Sulfobromophthalein disodium salt
  • HY-D0166
    Neutral Red
    		98.14%
    Neutral Red, a nitrogenous pH-indicator with a pKi of 6.8, is an indicator for the internal acidification of thylakoids. Neutral Red stains lysosomes red.
    Neutral Red
  • HY-P10932A
    pGk13a TFA
    pGk13a TFA is an azide (azide group can be combined with fluorophore)-containing amphiphilic membrane labeling probe. pGk13a TFA enables high-resolution imaging of cell membranes in the ultrastructural membrane expansion microscopy (umExM) technique, facilitating the observation of membrane-associated structures and proteins. pGk13a TFA can be used for neuronal structural studies.
    pGk13a TFA
Cat. No. Product Name / Synonyms Application Reactivity