BODIPY 665/676
Based on 1 publication(s) in Google Scholar
BODIPY 665/676 is a lipophilic radical-sensitive fluorescent probe that can be used to study radical-driven llipid peroxidation (Ex/Em=665/676 nm).
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.96%
- No. CAS: 164106-16-5
- Fòrmula: C29H23BF2N2
- Peso molecular:448.31
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Almacenamiento:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) BODIPY 665/676
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Actividad biológica
Descripciòn
In Vitro
Guide (The following is our recommended protocol. This protocol only provides guidance and should be modified according to your specific needs).
1. Preparation of BODIPY 665/676 working solution
1.1 Preparation of stock solution
Dissolve 1 mg BODIPY 665/676 in 223 μL anhydrous DMSO to prepare a 10 mM stock solution.
Note: It is recommended to aliquot the BODIPY 665/676 stock solution and store at -20°C or -80°C, protected from light.
1.2 Preparation of working solution
Dilute the stock solution with pre-warmed serum-free cell culture medium or PBS to prepare a 1-10 μM BODIPY 665/676 working solution.
Note: Please adjust the concentration of the BODIPY 665/676 working solution according to actual conditions and prepare fresh before use.
2. UV-induced free radical reaction
2.1 Medium-chain triglyceride (MCT) oil and linseed oil (LSO).
a. Use a spectrofluorometer to measure fluorescence in the UV-induced free radical reaction.
b. Irradiate control oil (oil containing 1 μM BODIPY 665/676) and sample oil (oil containing 1 μM BODIPY 665/676 and 5.7 μM di-tert-butyl peroxide (DTBP)) for a fixed irradiation time.
c. Compared with the non-irradiated control oil, the fluorescence intensity measured at 675 nm in the sample decreases relatively, while the fluorescence intensity measured at 580 nm increases relatively, which is used to quantify the degree of oxidation. Measurements are repeated three times.
Notes
1. Please adjust the concentration of the BODIPY 665/676 working solution according to actual conditions.
2. Samples containing unsaturated fatty acids should be handled under nitrogen protection.
3. This product is for research use only and is not intended for use in drugs, household, or other purposes.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
676
Excitation (Ex)
665
Chemical Information
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No. CAS 164106-16-5
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Appearance Solid
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Peso molecular 448.31
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Fòrmula C29H23BF2N2
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Color Brown to black
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SMILES
[F-][B+3]1([N]2=C(C=CC2=CC3=CC=C([N-]31)/C=C/C=C/C4=CC=CC=C4)/C=C/C=C/C5=CC=CC=C5)[F-]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvente y solubilidad
In Vitro:
DMSO : 50 mg/mL (111.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2306 mL | 11.1530 mL | 22.3060 mL | 55.7650 mL |
| 5 mM | 0.4461 mL | 2.2306 mL | 4.4612 mL | 11.1530 mL | |
| 10 mM | 0.2231 mL | 1.1153 mL | 2.2306 mL | 5.5765 mL | |
| 15 mM | 0.1487 mL | 0.7435 mL | 1.4871 mL | 3.7177 mL | |
| 20 mM | 0.1115 mL | 0.5576 mL | 1.1153 mL | 2.7882 mL | |
| 25 mM | 0.0892 mL | 0.4461 mL | 0.8922 mL | 2.2306 mL | |
| 30 mM | 0.0744 mL | 0.3718 mL | 0.7435 mL | 1.8588 mL | |
| 40 mM | 0.0558 mL | 0.2788 mL | 0.5576 mL | 1.3941 mL | |
| 50 mM | 0.0446 mL | 0.2231 mL | 0.4461 mL | 1.1153 mL | |
| 60 mM | 0.0372 mL | 0.1859 mL | 0.3718 mL | 0.9294 mL | |
| 80 mM | 0.0279 mL | 0.1394 mL | 0.2788 mL | 0.6971 mL | |
| 100 mM | 0.0223 mL | 0.1115 mL | 0.2231 mL | 0.5576 mL |
BODIPY 665/676 Related Classifications
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