Bacterial Sortase Substrate III, Abz/DNP TFA
Based on 1 Customer Validation
Bacterial Sortase Substrate III, Abz/DNP TFA is a fluorescent peptide substrate. Bacterial Sortase Substrate III, Abz/DNP TFA undergoes cleavage catalyzed by Staphylococcus aureus sortase A (SrtAΔN24) and Streptococcus pyogenes sortase A (SrtAΔN81), and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of the cell wall cross-bridge. Cleavage of this substrate can be detected at Ex/Em=320 nm/420 nm.
For research use only. We do not sell to patients.
- Purity: 98.04%
- Formula: C41H57N11O14.xC2HF3O2
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Biological Activity
Bacterial Sortase Substrate III, Abz/DNP TFA is catalyzed for hydrolysis by purified recombinant Staphylococcus aureus SrtAΔN24 with a Km of 17.5 μM[1].
Bacterial Sortase Substrate III, Abz/DNP TFA serves as a substrate for purified recombinant Streptococcus pyogenes SrtAΔN81-catalyzed transpeptidation with Gly3, generating detectable products via HPLC[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Formula C41H57N11O14.xC2HF3O2
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Color Light yellow to yellow
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Sequence
{Abz}-Leu-Pro-Glu-Thr-Gly-{Lys(Dnp)}-NH2
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Sequence Shortening
{Abz}-LPETG-{Lys(Dnp)}-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : ≥ 50 mg/mL
* "≥" means soluble, but saturation unknown.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
- Bacterial Sortase Substrate III, Abz/DNP
- Fluorescent Dye
- purified recombinant Staphylococcus aureus SrtAΔN24
- Staphylococcus aureus sortase A (SrtAΔN24)
- fluorogenic peptide substrate
- staphylococcus aureus infection
- sortase-catalyzed hydrolysis
- purified recombinant Streptococcus pyogenes SrtAΔN81
- Streptococcus pyogenes sortase A (SrtAΔN81)
- transpeptidation reactions
- Inhibitor
- inhibitor
- inhibit