Bacterial Sortase Substrate III, Abz/DNP
Bacterial Sortase Substrate III, Abz/DNP is a fluorescent peptide substrate. Bacterial Sortase Substrate III, Abz/DNP undergoes cleavage catalyzed by Staphylococcus aureus sortase A (SrtAΔN24) and Streptococcus pyogenes sortase A (SrtAΔN81), and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of the cell wall cross-bridge. Cleavage of this substrate can be detected at Ex/Em=320 nm/420 nm.
For research use only. We do not sell to patients.
- CAS No.: 2857862-12-3
- Formula: C41H57N11O14
- Molecular Weight:927.96
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Bacterial Sortase Substrate III, Abz/DNP is catalyzed for hydrolysis by purified recombinant Staphylococcus aureus SrtAΔN24 with a Km of 17.5 μM[1].
Bacterial Sortase Substrate III, Abz/DNP serves as a substrate for purified recombinant Streptococcus pyogenes SrtAΔN81-catalyzed transpeptidation with Gly3, generating detectable products via HPLC[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 2857862-12-3
-
Molecular Weight 927.96
-
Formula C41H57N11O14
-
Sequence
{Abz}-Leu-Pro-Glu-Thr-Gly-{Lys(Dnp)}-NH2
-
Sequence Shortening
{Abz}-LPETG-{Lys(Dnp)}-NH2
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
|---|---|---|
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
|---|---|
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Bacterial Sortase Substrate III, Abz/DNP Related Classifications
HY-P1883 Related Classifications
Powered by Bioz
- Bacterial Sortase Substrate III, Abz/DNP
- 2857862-12-3
- Fluorescent Dye
- purified recombinant Staphylococcus aureus SrtAΔN24
- Staphylococcus aureus sortase A (SrtAΔN24)
- fluorogenic peptide substrate
- staphylococcus aureus infection
- sortase-catalyzed hydrolysis
- purified recombinant Streptococcus pyogenes SrtAΔN81
- Streptococcus pyogenes sortase A (SrtAΔN81)
- transpeptidation reactions
- Inhibitor
- inhibitor
- inhibit