Xylene Cyanol FF
Based on 1 Customer Validation
Xylene Cyanol FF is an acid triphenylmethane dye. Xylene Cyanol FF can be used for histochemical staining of hemoglobin peroxidase or as a tracking dye for DNA sequencing in electrophoresis. Xylene Cyanol FF will be catalyzed by Fe and Al to accelerate oxidation under the addition of double oxidant hydrogen peroxide and potassium periodate. Xylene Cyanol FF thus enables the spectrophotometric determination of Fe and Al in the solution to be tested.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 94.69%
- CAS. Nr.: 2650-17-1
- Formel: C25H27N2NaO6S2
- Molecular Weight:538.61
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Speicherung:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
Xylene Cyanol FF tracks DNA on different polyacrylamide gels. The operation is as follows[1]:
1. Used for denaturing gel.
(1) Prepare gel: Contain 10% acrylamide (19:1, acrylamide:bisacrylamide), and 8.3 M urea. The operating condition is 55°C.
(2) Prepare electrophoresis buffer: 89 mM Tris, HCl, pH 8.0, 89 mM boric acid, 2 mM EDTA (TBE).
(3) Prepare loading buffer: 10 mM NaOH, 1 mM EDTA, and 0.1% Xylene Cyanol FF.
(4) Gel is run at 70 W (50 V/cm, constant power) on an electrophoresis unit, or 60 °C (31 V/cm, constant voltage).
(5) Dry the gel on Whatman 3MM paper and expose to X-ray film. Exposure time up to 15 hours.
2. Used for denaturing gel.
(1) Prepare the gel: Contain 8% acrylamide (19:1, acrylamide:bisacrylamide).
(2) Prepare DNA suspension: containing 40 mM TrisβHCl, pH 8.0, 20 mM acetic acid, 2 mM EDTA and 12.5 mM magnesium acetate (TAEMg).
(3) Boil the DNA suspension and cool slowly to 16 °C.
(4) Prepare staining solution: containing TAEMg, 50% glycerol, 0.02% bromophenol blue and 0.02% Xylene Cyanol FF. Bring up the sample to a final volume of 10 μL with this staining solution.
(5) The gel is run on a Hoefer SE-600 gel electrophoresis unit at 11 V/cm at 16 °C and exposed to X-ray film for up to 15 hours or stained with Stainsall (HY-D0987).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 2650-17-1
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Appearance Solid
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Molecular Weight 538.61
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Formel C25H27N2NaO6S2
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Color Dark green to black
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SMILES
O=S(C1=CC=C(/C(C2=CC=C(NCC)C(C)=C2)=C3C=C(C)/C(C=C/3)=N\CC)C(S(=O)(O)=O)=C1)(O[Na])=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Lösungsmittel & Löslichkeit
In Vitro:
H2O : 10 mg/mL (18.57 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Reinheit & Dokumentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.8566 mL | 9.2832 mL | 18.5663 mL | 46.4158 mL |
| 5 mM | 0.3713 mL | 1.8566 mL | 3.7133 mL | 9.2832 mL | |
| 10 mM | 0.1857 mL | 0.9283 mL | 1.8566 mL | 4.6416 mL | |
| 15 mM | 0.1238 mL | 0.6189 mL | 1.2378 mL | 3.0944 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Xylene Cyanol FF Related Classifications
HY-D0945 Related Classifications
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