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Stains-All 

Cat. No.: HY-D0987 Purity: 98.02%
Handling Instructions

Stains-All is a cationic carbocyanine dye.

For research use only. We do not sell to patients.

Stains-All Chemical Structure

Stains-All Chemical Structure

CAS No. : 7423-31-6

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 103 In-stock
Estimated Time of Arrival: December 31
5 mg USD 84 In-stock
Estimated Time of Arrival: December 31
10 mg   Get quote  
50 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

Stains-All is a cationic carbocyanine dye.

In Vitro

Almost all of the proteins found in skeletal muscle extracts, including the Ca2++Mg2+-ATPase and the 53,000-Da glycoprotein of the sarcoplasmic reticulum, are stained red or pink with Stains-all. Calsequestrin, the 1 60,000-Da glycoprotein, and 170,000-Da protein are stained blue with Stains-all. The ratio of Stains-all staining (measured at 615 nm) to that of Coomassie blue staining (measured at 575 nm) is 1.3 for calsequestrin, 2.0 for calmodulin, 1.4 for troponin C, and 2.2 for S-100. Therefore, in addition to differentially staining these Ca2+-binding proteins blue, Stains-all is a more sensitive stain for these Ca2+-binding proteins than is Coomassie blue[1].

Storage

4°C, protect from light

Solvent & Solubility
In Vitro: 

DMSO : 65 mg/mL (116.16 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7871 mL 8.9353 mL 17.8705 mL
5 mM 0.3574 mL 1.7871 mL 3.5741 mL
10 mM 0.1787 mL 0.8935 mL 1.7871 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[1]

Slab gels are fixed overnight with 25% isopropyl alcohol and washed exhaustively in 25% isopropyl alcohol to remove SDS. The gels are then stained in the dark for at least 48 h with 0.0025% Stains-all, 25% isopropyl alcohol, 7.5% formamide, and 30 mM Tris base, pH 8.8. The interaction of Stains-all with various Ca2+-binding proteins is also studied in aqueous solution. The standard solution contains 10 mM Tris base, pH 8.8, 0.001% Stains-all, and 0.1% formamide. Ca2+-binding proteins (0.5 to 12 μg) are added to 1.0 mL of solution and then incubated at room temperature in the dark for 30 min. The absorbance at 600 nm is then measured against a control solution, containing no protein, using a spectrophotometer[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

559.58

Formula

C₃₀H₂₇BrN₂S₂

CAS No.

7423-31-6

SMILES

CC(/C=C1SC2=CC=C3C=CC=CC3=C2N\1CC)=C\C4=[N+](CC)C5=C6C=CC=CC6=CC=C5S4.[Br-]

Shipping

Room temperature in continental US; may vary elsewhere

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Stains-All
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Cat. No.: HY-D0987