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  4. Green DND-26

Green DND-26 is a green fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 504/511 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and generally gather on spherical organelles. Green DND-26 is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes.

For research use only. We do not sell to patients.

Green DND-26 Chemical Structure

Green DND-26 Chemical Structure

CAS No. : 220524-71-0

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5 mg USD 260 In-stock
10 mg USD 420 In-stock
25 mg USD 850 In-stock
50 mg USD 1360 In-stock
100 mg USD 2200 In-stock
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Based on 1 publication(s) in Google Scholar

Green DND-26 Related Antibodies

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1 Publications Citing Use of MCE Green DND-26

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Description

Green DND-26 is a green fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 504/511 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and generally gather on spherical organelles. Green DND-26 is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes[1].

In Vitro

Preparation of Green DND-26 working solution
1. Preparation of the stock solution
1.1 Restore the solution to the room temperature and concentrate it to the bottom of the tube by instantaneous centrifugation.
1.2 Dilute 1 mM storage solution to the working solution using medium or appropriate buffer (such as PBS) . The recommended working solution is 50-100 nM.
Note: Please adjust the concentration of Green DND-26 working solution according to the actual situation, and use it now.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Green DND-26 working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
Storage
-20°C save, Protect from light

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Green DND-26 Related Antibodies
Molecular Weight

362.23

Formula

C18H25BF2N4O
CAS No.
Appearance

Solid

Color

Orange to red

Emission (Em)

512
Excitation (Ex)

482
SMILES

O=C(CCC1=CC=C2C=C3C(C)=CC(C)=[N]3[B+3]([F-])([N-]21)[F-])NCCN(C)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (345.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7607 mL 13.8034 mL 27.6068 mL
5 mM 0.5521 mL 2.7607 mL 5.5214 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7607 mL 13.8034 mL 27.6068 mL 69.0169 mL
5 mM 0.5521 mL 2.7607 mL 5.5214 mL 13.8034 mL
10 mM 0.2761 mL 1.3803 mL 2.7607 mL 6.9017 mL
15 mM 0.1840 mL 0.9202 mL 1.8405 mL 4.6011 mL
20 mM 0.1380 mL 0.6902 mL 1.3803 mL 3.4508 mL
25 mM 0.1104 mL 0.5521 mL 1.1043 mL 2.7607 mL
30 mM 0.0920 mL 0.4601 mL 0.9202 mL 2.3006 mL
40 mM 0.0690 mL 0.3451 mL 0.6902 mL 1.7254 mL
50 mM 0.0552 mL 0.2761 mL 0.5521 mL 1.3803 mL
60 mM 0.0460 mL 0.2301 mL 0.4601 mL 1.1503 mL
80 mM 0.0345 mL 0.1725 mL 0.3451 mL 0.8627 mL
100 mM 0.0276 mL 0.1380 mL 0.2761 mL 0.6902 mL
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Green DND-26 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Green DND-26220524-71-0Green DND26Green DND 26Fluorescent Dyeacidic lysosomeslamellar bodiesAT2 cellslungInhibitorinhibitorinhibit

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