Green DND-26 (solution) 

Green DND-26 (solution) is a green fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 504/511 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and generally gather on spherical organelles. Green DND-26 is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes^[1].
Solvent and concentration: DMSO: 2 mM

Guidelines (The following are our recommended protocols. This protocol is for reference only and should be modified according to your specific needs.)
Preparation of Green DND-26 working solution
1. Preparation of the stock solution
1.1 Restore the solution to the room temperature and concentrate it to the bottom of the tube by instantaneous centrifugation.
1.2 Dilute storage solution to the working solution using medium or appropriate buffer (such as PBS) . The recommended working solution is 50-100 nM.
Note: Please adjust the concentration of Green DND-26 working solution according to the actual situation, and use it now.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Green DND-26 working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
Storage
-20°C save, Protect from light

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

362.23

C₁₈H₂₅BF₂N₄O

220524-71-0

O=C(CCC1=CC=C2C=C3C(C)=CC(C)=[N]3[B+3]([F-])([N-]21)[F-])NCCN(C)C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Sun B, et al. Acid-Activatable Transmorphic Peptide-Based Nanomaterials for Photodynamic Therapy. Angew Chem Int Ed Engl. 2020;59(46):20582-20588.  [Content Brief]

  [2]. Van der Velden JL, et al. LysoTracker is a marker of differentiated alveolar type II cells. Respir Res. 2013;14(1):123. Published 2013 Nov 11.  [Content Brief]