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  4. Basic green 4

Basic green 4 (Malachite green) is a cationic dye. Basic green 4 also is an N-methylated diaminotriphenylmethane dye to be used for coloring purpose.

For research use only. We do not sell to patients.

Basic green 4 Chemical Structure

Basic green 4 Chemical Structure

CAS No. : 569-64-2

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Based on 1 publication(s) in Google Scholar

Basic green 4 Related Antibodies

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Description

Basic green 4 (Malachite green) is a cationic dye. Basic green 4 also is an N-methylated diaminotriphenylmethane dye to be used for coloring purpose[1].

Molecular Weight

364.91

Formula

C23H25ClN2
CAS No.
Appearance

Solid

Color

Green to black

SMILES

C/[N+](C)=C1C=C/C(C=C/1)=C(C2=CC=C(N(C)C)C=C2)\C3=CC=CC=C3.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (274.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 3.85 mg/mL (10.55 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7404 mL 13.7020 mL 27.4040 mL
5 mM 0.5481 mL 2.7404 mL 5.4808 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

COA (249 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.7404 mL 13.7020 mL 27.4040 mL 68.5100 mL
5 mM 0.5481 mL 2.7404 mL 5.4808 mL 13.7020 mL
10 mM 0.2740 mL 1.3702 mL 2.7404 mL 6.8510 mL
DMSO 15 mM 0.1827 mL 0.9135 mL 1.8269 mL 4.5673 mL
20 mM 0.1370 mL 0.6851 mL 1.3702 mL 3.4255 mL
25 mM 0.1096 mL 0.5481 mL 1.0962 mL 2.7404 mL
30 mM 0.0913 mL 0.4567 mL 0.9135 mL 2.2837 mL
40 mM 0.0685 mL 0.3426 mL 0.6851 mL 1.7128 mL
50 mM 0.0548 mL 0.2740 mL 0.5481 mL 1.3702 mL
60 mM 0.0457 mL 0.2284 mL 0.4567 mL 1.1418 mL
80 mM 0.0343 mL 0.1713 mL 0.3426 mL 0.8564 mL
100 mM 0.0274 mL 0.1370 mL 0.2740 mL 0.6851 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Basic green 4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Basic green 4569-64-2Malachite greenBasic green4Basic green-4Fluorescent Dyecationic dyeN-methylated diaminotriphenylmethane dyecoloring purposeInhibitorinhibitorinhibit

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