1. Apoptosis
  2. Caspase
  3. Z-DEVD-FMK

Z-DEVD-FMK 

製品番号: HY-12466 純度: >98.0%
取扱説明書

Z-DEVD-FMK is a specific and irreversible caspase-3 inhibitor with IC50 of 18 μM.

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Z-DEVD-FMK 構造式

Z-DEVD-FMK 構造式

CAS 番号 : 210344-95-9

容量 価格(税別) 在庫状況 数量
10 mM * 1  mL in DMSO USD 263 在庫あり
Estimated Time of Arrival: December 31
1 mg USD 66 在庫あり
Estimated Time of Arrival: December 31
5 mg USD 179 在庫あり
Estimated Time of Arrival: December 31
10 mg USD 299 在庫あり
Estimated Time of Arrival: December 31
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

カスタマーレビュー

Based on 23 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Z-DEVD-FMK purchased from MCE. Usage Cited in: Apoptosis. 2016 Feb;21(2):130-42.

    (a) CPT-11 induces cell cycle arrest and apoptosis in RAW 264.7macrophages. a.Western blot analysis of cleaved caspase-3 and PARP levels. (b) CPT-11 induced cell cycle arrest and apoptosis in mouse peritoneal macrophages. Western blot analysis of cleaved caspase-3 and PARP levels in peritoneal macrophages isolated from vehicleand CPT-11-administered mice. (c) Caspase-3 inhibitor z-DEVD-fmk (ZD) attenuates CPT-11-induced loss of GATA6+ peritoneal macrophages in mice. Western blot analy

    Z-DEVD-FMK purchased from MCE. Usage Cited in: Acta Pharm Sin B. 2020 Jul.

    Hepa1-6 cells are treated with miltirone (40 μM) in the absence or presence of Z-DEVD-FMK (20 μM) for 24 h, total cellular extracts are prepared and subjected to Western blotting analyses using antibodies against caspase 3, GSDME, and GADPH.
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    製品説明

    Z-DEVD-FMK is a specific and irreversible caspase-3 inhibitor with IC50 of 18 μM.

    IC50 & Target[1]

    Caspase-3

    18 μM (IC50)

    体外実験

    N27 cells are exposed to MPP+ in the absence or presence of 50 μM Z-DIPD-FMK or 100 μM Z-DEVD-FMK or 50 μM Z-LEHD-FMK and then caspase-9 and caspase-3 enzymatic activities are determined by enzymatic assay at 12 and 24 h following exposure, respectively. Exposure to 300 μM MPP+ for 24 h in N27 cells results in an approximately 2.5-fold increase in caspase-3 enzyme activity. MPP+-induced increases in caspase-3 enzyme activity are significantly blocked by 50 μM Z-DIPD-FMK, 100 μM Z-DEVD-FMK, and 50 μM Z-LEHD-FMK[1].

    体内実験

    Early Z-DEVD-FMK (160 ng) treatment improves motor and cognitive function after traumatic CNS injury induced by severe controlled cortical impact (CCI) in the mouse[2]. Treatment with Z-DEVD-FMK (160 ng) significantly improves neurological outcome when compared with traumatized animals treated with DMSO vehicle (p<0.01)[3].

    分子量

    668.66

    分子式

    C₃₀H₄₁FN₄O₁₂

    CAS 番号

    210344-95-9

    SMILES

    O=C(N[[email protected]@H](C(C)C)C(N[[email protected]](C(CF)=O)CC(OC)=O)=O)[[email protected]](CCC(OC)=O)NC([[email protected]](CC(OC)=O)NC(OCC1=CC=CC=C1)=O)=O

    輸送条件

    Room temperature in continental US; may vary elsewhere.

    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 33.33 mg/mL (49.85 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4955 mL 7.4776 mL 14.9553 mL
    5 mM 0.2991 mL 1.4955 mL 2.9911 mL
    10 mM 0.1496 mL 0.7478 mL 1.4955 mL
    *Please refer to the solubility information to select the appropriate solvent.
    体内:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (3.74 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (3.74 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (3.74 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    参考文献
    細胞実験
    [1]

    N27 cells and primary mesencephalic neurons are exposed to either 10-100 μM 6-OHDA or 10-300 μM MPP+ in the presence or absence of 0.1-50 μM Z-DIPD-FMK or 0.1-100 μM Z-DEVD-FMK or 50 μM Z-IETD-FMK or Z-LEHD-FMK for the duration of the experiment. N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/mL MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader[1].

    MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

    動物実験
    [2][3]

    Mice[2]
    Male C57Bl/6 mice (20-25 g) are used. For treatment with Z-DEVD-fmk or vehicle after CCI, mice are placed in a stereotaxic apparatus, and the CCI wound is reopened for intracerebroventricular injection. Either Z-DEVD-FMK (160 ng in 2 μL DMSO), or DMSO vehicle is injected over a 5-minute period.
    Rats[3]
    Male Sprague Dawley rats (425±25 g) are used. DMSO (5 μL) vehicle or Z-DEVD-FMK (160 ng in 5 μL of DMSO) is administered at a controlled rate of 0.5 μL/min via an infusion pump at 30 min before and at 6 and 24 hr after TBI. At the designated time periods after injury, animals are decapitated under NSC 10816 anesthesia (100 mg/kg, i.p.), and the brains are removed rapidly and dissected. Sham-operated (control) animals received anesthesia and surgery but are not subjected to trauma. Tissue samples are collected 1, 4, 12, 24, and 72 hr after TBI. Samples are frozen on dry ice and kept at −85°C.

    MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。

    参考文献
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    Keywords:

    Z-DEVD-FMKCaspaseInhibitorinhibitorinhibit

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    製品名:
    Z-DEVD-FMK
    製品番号:
    HY-12466
    数量:
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