2. Immunology/Inflammation Apoptosis
  3. Interleukin Related IFNAR TNF Receptor Apoptosis
  4. Chamissonolide

Chamissonolide is a sesquiterpene lactone with cytotoxic, anti-inflammatory and trypanocidal activities. Chamissonolide reduces the mRNA levels of IL-2, IFN-γ, GM-CSF, iNOS and TNF-α, and upregulates the mRNA level of NF-ATc. Chamissonolide decreases the population of naturally occurring apoptotic cells. Chamissonolide can be used in research related to tumors, African trypanosomiasis and Chagas disease.

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Chamissonolide

Chamissonolide Chemical Structure

CAS No. : 78853-98-2

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Chamissonolide Related Antibodies

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Description

Chamissonolide is a sesquiterpene lactone with cytotoxic, anti-inflammatory and trypanocidal activities. Chamissonolide reduces the mRNA levels of IL-2, IFN-γ, GM-CSF, iNOS and TNF-α, and upregulates the mRNA level of NF-ATc. Chamissonolide decreases the population of naturally occurring apoptotic cells. Chamissonolide can be used in research related to tumors, African trypanosomiasis and Chagas disease[1][2][3].

IC50 & Target[1]

IL-2

 

In Vitro

Chamissonolide (Compound 3) exhibits a baseline cytotoxicity IC50 of 2.3 μM against KB epithelial tumor cells following 72 h of treatment[1].
Chamissonolide (5-20 μM; 2.5-20 h) downregulates the mRNA levels of specific pro-inflammatory cytokines (IL-2, IFN-γ, GM-CSF, iNOS, TNF-α) in PMA (HY-18739)-stimulated human peripheral blood mononuclear cells (PBMCs) and CD4+ Jurkat T cells, while upregulating the mRNA level of NF-ATc[2].
Chamissonolide (0.5-80 μM; 2.5-72 h) exhibits an IC50 of 13 μM against CD4+ Jurkat T cells after a 72-hour treatment[2].
Chamissonolide (10-20 μM; 20 h) reduces spontaneous apoptosis in CD4+ Jurkat T cells[2].
Chamissonolide (compound 4) (0.123-90 μg/mL; 72-96 h) inhibits the growth of Trypanosoma brucei rhodesiense STIB 900 with an IC50 value of 9.275 μM; it inhibits the Trypanosoma cruzi Tulahuen strain C2C4 with an IC50 value of 45.833 μM; meanwhile, it exhibits cytotoxicity against rat skeletal muscle myoblasts L-6 with an IC50 value of 15.191 μM[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chamissonolide Related Antibodies

Real Time qPCR[2]

Cell Line: PMA-stimulated CD4+ Jurkat human leukemia T cells
Concentration: 10 μM
Incubation Time: 2.5 h; 5 h; 20 h
Result: Down-regulated PMA-induced pro-inflammatory cytokine mRNA levels similarly to its effects in PBMCs at 10 μM.
Did not alter mRNA levels of house-keeping genes (GAP-DH, β-actin), cell homeostasis factors (cyclin D1, bcl-2), or I-κBα.
Significantly up-regulated NF-ATc mRNA levels after 20 hours of treatment at 10 μM.

Cell Viability Assay[2]

Cell Line: CD4+ Jurkat human leukemia T cells, PBMCs
Concentration: 0.5 μM; 1 μM; 2.5 μM; 5 μM; 10 μM; 20 μM; 40 μM; 80 μM
Incubation Time: 2.5 h; 20 h; 72 h
Result: Was not significantly cytotoxic against PBMCs at concentrations up to 40 μM at all tested time points.
Showed cytotoxicity in Jurkat T cells first observed between 20 and 72 hours at higher concentrations, with an IC50 of 13 μM after 72 hours.
Did not significantly reduce viability of either cell type at 10 μM over 72 hours, and showed cytoprotective effects in PBMCs after 72 hours as indicated by increased WST-1 cleavage.

Apoptosis Analysis[2]

Cell Line: CD4+ Jurkat human leukemia T cells
Concentration: 10 μM; 20 μM
Incubation Time: 20 h
Result: Reduced the number of apoptotic Jurkat T cells compared to untreated controls at 10 μM, with cell morphology identical to normal control populations.
Did not induce caspase-8 or caspase-3 activity at concentrations up to 20 μM.
Molecular Weight

324.37

Formula

C17H24O6
CAS No.
SMILES

C[C@]12[C@@]([C@@H](C[C@]3([H])[C@](C(C(O3)=O)=C)([H])[C@@H]2O)C)([H])[C@H](C[C@H]1O)OC(C)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Chamissonolide Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Chamissonolide78853-98-2Interleukin RelatedIFNARTNF ReceptorApoptosisILInterferon-α/β receptorInterferon-alpha/beta receptor Tumor Necrosis Factor ReceptorTNFRhuman PBMCsCD4+ T cellsL-6 rat skeletal myoblastsTrypanosoma brucei rhodesienseNF-ATciNOSCD4+ Jurkat T cellsTrypanosoma cruziKB epithelial tumor cellsArnica speciesInhibitorinhibitorinhibit

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