Guanidine thiocyanate

Name: Guanidine thiocyanate
Brand: MedChemExpress
SKU: HY-D0841
Rating: 5.0 (3 reviews)

Cat. No.: HY-D0841 Purity: 99.96%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Guanidine thiocyanate is a strong protein denaturant and potent inhibitor of nucleases. Guanidinium thiocyanate is a nucleic acid protector in the extraction of DNA and RNA from cells. Guanidine thiocyanate is a common component of buffers used for nucleic acid extraction.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 593-84-0

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
25 g	해외재고보유	Estimated Time of Arrival: December 31
50 g	해외재고보유	Estimated Time of Arrival: December 31
100 g	해외재고보유	Estimated Time of Arrival: December 31
500 g	해외재고보유	Estimated Time of Arrival: December 31
> 100 g	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 3 publication(s) in Google Scholar

Other Forms of Guanidine thiocyanate:

Guanidine hydrochloride In-stock
Guanidine thiocyanate (Standard) 견적 받기

3 Publications Citing Use of MCE Guanidine thiocyanate

Bio/Physico-chemical Assay

: Guanidine thiocyanate purchased from MedChemExpress. Usage Cited in: Virus Res. 2020 Jul 2;283:197974. [Abstract]; RD cells were pretreated with DMSO, NMS-873 (8 μM), DMSO plus GuHCl (2 μM) or NMS-873 plus GuHCl for 30 min. Then cells were transfected with EVA71 replicon RNA (0.1 μg/well) or infected with EVA71 pesudovirus (25 μl/well). Luciferase activity was quantified at different time points post transfection or infection.

: Guanidine thiocyanate purchased from MedChemExpress. Usage Cited in: Virol J. 2018 Jan 3;15(1):1. [Abstract]; Immunofluorescence analysis is performed on RD and A549 cells infected with EV71 at an MOI of 10 (in RD) and 50 (in A549) in the presence or absence of 2.5 mM GnHCl.

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Guanidine thiocyanate based-nucleic acid extraction buffers can effectively inactivate high-consequence enveloped viruses, such as Ebola virus, Marburg virus, and Rift Valley Fever virus, as well as West Nile virus, influenza, Venezuelan equine encephalitis virus, and recently, SARS-CoV-2^[1].
Guanidine thiocyanate-based nucleic acid extraction buffers are an effective means of inactivating poliovirus infectious materials and potentially infectious materials^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

118.16

화학식

C₂H₆N₄S

CAS No.

593-84-0

Appearance

Solid

Color

White to off-white

SMILES

SC#N.NC(N)=N

Initial Source

Endogenous metabolite

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 100 mg/mL (846.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (846.31 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.4631 mL	42.3155 mL	84.6310 mL
5 mM	1.6926 mL	8.4631 mL	16.9262 mL
10 mM	0.8463 mL	4.2316 mL	8.4631 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (21.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (21.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (21.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

순도&문서

Purity: 99.96%

Select Batch:

Data Sheet (272 KB) SDS (615 KB)

COA (247 KB) HNMR (245 KB) RP-HPLC (260 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Michelle J Honeywood, et al. Use of guanidine thiocyanate-based nucleic acid extraction buffers to inactivate poliovirus in potentially infectious materials. J Virol Methods. 2021 Nov:297:114262. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	8.4631 mL	42.3155 mL	84.6310 mL	211.5775 mL
	5 mM	1.6926 mL	8.4631 mL	16.9262 mL	42.3155 mL
	10 mM	0.8463 mL	4.2316 mL	8.4631 mL	21.1578 mL
	15 mM	0.5642 mL	2.8210 mL	5.6421 mL	14.1052 mL
	20 mM	0.4232 mL	2.1158 mL	4.2316 mL	10.5789 mL
	25 mM	0.3385 mL	1.6926 mL	3.3852 mL	8.4631 mL
	30 mM	0.2821 mL	1.4105 mL	2.8210 mL	7.0526 mL
	40 mM	0.2116 mL	1.0579 mL	2.1158 mL	5.2894 mL
	50 mM	0.1693 mL	0.8463 mL	1.6926 mL	4.2316 mL
	60 mM	0.1411 mL	0.7053 mL	1.4105 mL	3.5263 mL
	80 mM	0.1058 mL	0.5289 mL	1.0579 mL	2.6447 mL
	100 mM	0.0846 mL	0.4232 mL	0.8463 mL	2.1158 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.