2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Guanidine thiocyanate

Guanidine thiocyanate  (Synonyms: グアニジンチオシアン酸塩)

製品番号: HY-D0841 純度: 99.96%
COA 取扱説明書 Technical Support

Guanidine thiocyanate is a strong protein denaturant and potent inhibitor of nucleases. Guanidinium thiocyanate is a nucleic acid protector in the extraction of DNA and RNA from cells. Guanidine thiocyanate is a common component of buffers used for nucleic acid extraction.

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CAS 番号 : 593-84-0

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 27 在庫あり
Solution
10 mM * 1 mL in DMSO USD 27 在庫あり
Solid
25 g $25 在庫あり
50 g $35 在庫あり
100 g $45 在庫あり
500 g $90 在庫あり
> 100 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 3 publication(s) in Google Scholar

Other Forms of Guanidine thiocyanate:

Guanidine thiocyanate 関連抗体

Top Publications Citing Use of Products

    Guanidine thiocyanate purchased from MedChemExpress. Usage Cited in: Virus Res. 2020 Jul 2;283:197974.  [Abstract]

    RD cells were pretreated with DMSO, NMS-873 (8 μM), DMSO plus GuHCl (2 μM) or NMS-873 plus GuHCl for 30 min. Then cells were transfected with EVA71 replicon RNA (0.1 μg/well) or infected with EVA71 pesudovirus (25 μl/well). Luciferase activity was quantified at different time points post transfection or infection.

    Guanidine thiocyanate purchased from MedChemExpress. Usage Cited in: Virol J. 2018 Jan 3;15(1):1.  [Abstract]

    Immunofluorescence analysis is performed on RD and A549 cells infected with EV71 at an MOI of 10 (in RD) and 50 (in A549) in the presence or absence of 2.5 mM GnHCl.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

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    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Guanidine thiocyanate is a strong protein denaturant and potent inhibitor of nucleases. Guanidinium thiocyanate is a nucleic acid protector in the extraction of DNA and RNA from cells. Guanidine thiocyanate is a common component of buffers used for nucleic acid extraction[1].

    IC50 & Target

    Human Endogenous Metabolite

     

    体外実験

    Guanidine thiocyanate based-nucleic acid extraction buffers can effectively inactivate high-consequence enveloped viruses, such as Ebola virus, Marburg virus, and Rift Valley Fever virus, as well as West Nile virus, influenza, Venezuelan equine encephalitis virus, and recently, SARS-CoV-2[1].
    Guanidine thiocyanate-based nucleic acid extraction buffers are an effective means of inactivating poliovirus infectious materials and potentially infectious materials[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Guanidine thiocyanate 関連抗体
    分子量

    118.16

    分子式

    C2H6N4S
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    SC#N.NC(N)=N
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (846.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (846.31 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 8.4631 mL 42.3155 mL 84.6310 mL
    5 mM 1.6926 mL 8.4631 mL 16.9262 mL
    10 mM 0.8463 mL 4.2316 mL 8.4631 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (21.16 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (21.16 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.96%

    COA (247 KB) HNMR (245 KB) RP-HPLC (260 KB) MS (69 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 8.4631 mL 42.3155 mL 84.6310 mL 211.5775 mL
    5 mM 1.6926 mL 8.4631 mL 16.9262 mL 42.3155 mL
    10 mM 0.8463 mL 4.2316 mL 8.4631 mL 21.1578 mL
    15 mM 0.5642 mL 2.8210 mL 5.6421 mL 14.1052 mL
    20 mM 0.4232 mL 2.1158 mL 4.2316 mL 10.5789 mL
    25 mM 0.3385 mL 1.6926 mL 3.3852 mL 8.4631 mL
    30 mM 0.2821 mL 1.4105 mL 2.8210 mL 7.0526 mL
    40 mM 0.2116 mL 1.0579 mL 2.1158 mL 5.2894 mL
    50 mM 0.1693 mL 0.8463 mL 1.6926 mL 4.2316 mL
    60 mM 0.1411 mL 0.7053 mL 1.4105 mL 3.5263 mL
    80 mM 0.1058 mL 0.5289 mL 1.0579 mL 2.6447 mL
    100 mM 0.0846 mL 0.4232 mL 0.8463 mL 2.1158 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Guanidine thiocyanate Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Guanidine593-84-0Endogenous MetaboliteChaotropic agentProtein denaturantNucleic acid protectorDNA extractionRNA extractionInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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