Cat. No.: HY-18341B

L-Thyroxine sodium

(Synonyms: Levothyroxine sodium; T4 sodium)

12 Cited Publications

Customer Review

Based on 12 publication(s) in Google Scholar

L-Thyroxine sodium (Levothyroxine sodium) is a synthetic hormone for the research of hypothyroidism. DIO enzymes convert biologically active thyroid hormone (Triiodothyronine,T3) from L-Thyroxine (T4).

For research use only. We do not sell to patients.

Purity: 99.75%
CAS No.: 55-03-8
Formula: C₁₅H₁₀I₄NNaO₄
Molecular Weight:798.85

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

L-Thyroxine sodium Chemical Structure

CAS No.: 55-03-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vivo
Chemical Information
Publications (12)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vivo

Deiodinases (DIOs), which catalyse the conversion of thyroxine (pro-hormone) to the active thyroid hormone, are associated with thyroid stimulating hormone (TSH) levels. DIO1 and DIO2 catalyze activation of thyroid hormone secretion in contrast to DIO3 playing role inactivation of the secretion. Activities of DIO1 and DIO2 play pivotal role in the negative feedback regulation of pituitary TSH secretion^[1]. L-Thyroxine (T4) and Triiodothyronine (T3) hormones are known to modulate the expression of ionic channels, pumps and regulatory contractile proteins. Moreover, thyroid hormones have been shown to influence calcium homeostasis and flux responsible for excitation and contractility, with L-Thyroxine and Triiodothyronine modulating its pharmacological control and secretion. In rats fed 12 weeks with the iodine-free diet, a significant decrease in the levels of both Triiodothyronine and L-Thyroxine is observed when compared to the control group fed with standard diet (p<0.001). In the group treated with low doses of L-Thyroxine, an increase in L-Thyroxine levels is observed (p=0.02) while Triiodothyronine levels remain virtually similar to the control group (p=0.19). Rats treated with high doses of L-Thyroxine display a significant increase in both Triiodothyronine and L-Thyroxine circulating concentrations compared to the non-treated hypothyroid group (p<0.001 and p=0.004, respectively) and a significant increase in L-Thyroxine levels when compared to the control values (p=0.03)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 55-03-8
Appearance Solid
Molecular Weight 798.85
Formula C₁₅H₁₀I₄NNaO₄
Color Off-white to light yellow
SMILES

N[C@@H](CC1=CC(I)=C(OC2=CC(I)=C(O)C(I)=C2)C(I)=C1)C(O[Na])=O
Synonyms

Levothyroxine sodium; T4 sodium
Structure Classification
- Others
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (12)

All In Vivo Efficacy StudyHistological Imaging/StainingIn Vivo Imaging

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Journal Impact Factor

Cell

Restoring circadian rhythms in the hypothalamic paraventricular nucleus reverses aging biomarkers and extends lifespan in male mice. [Abstract]

2026 Apr 2;189(7):2007-2023.e20. PMID: 41785851
Nat Immunol

A method for predicting drugs that can boost the efficacy of immune checkpoint blockade. [Abstract]

2024 Apr;25(4):659-670. PMID: 38499799
Cell Rep Med

Profiling the metabolome of uterine fluid for early detection of ovarian cancer. [Abstract]

2023 Jun 20;4(6):101061. PMID: 37267943
EMBO J

Whole-body replacement of larval myofibers generates permanent adult myofibers in zebrafish. [Abstract]

2024 Aug;43(15):3090-3115. PMID: 38839992

L-Thyroxine sodium purchased from MedChemExpress. Usage Cited in: EMBO J. 2024 Aug;43(15):3090-3115. [Abstract]

Timeline and three different Thyroxine hormone (T4) treatment schemes. RT-qPCR analysis of the whole-animal myofiber loss for each of the schemes.
Europace

Fto-mediated m6A demethylation of Lox drives atrial fibrosis and promotes atrial fibrillation in a murine model of hyperthyroidism. [Abstract]

2026 Mar 30;28(4):euag047. PMID: 41812233
J Drug Deliv Sci Technol

2023 Sep 28, 105008.
Stem Cell Rev Rep

Satellite Glial Cells Give Rise to Nociceptive Sensory Neurons. [Abstract]

2021 Jun;17(3):999-1013. PMID: 33389681
Sci Rep

The iodide transporter Slc26a7 impacts thyroid function more strongly than Slc26a4 in mice. [Abstract]

2022 Jul 4;12(1):11259. PMID: 35788623

L-Thyroxine sodium purchased from MedChemExpress. Usage Cited in: Sci Rep. 2022 Jul 4;12(1):11259. [Abstract]

Growth curve of male wild type (WT), Slc26a7^+/−, Slc26a7^−/−, and Slc26a7^-/− mice receiving L-Thyroxine (drinking water containing 0.39 mg/L L-Thyroxine) replacement from day 21 with a low iodine diet. Note that Slc26a7^−/− mice without thyroid hormone replacement did not survive.
Clin Exp Med

The impact of hyperthyroidism on the hematopoietic system. [Abstract]

2025 Dec 20. PMID: 41420729
Dis Model Mech

Compromised COPII vesicle trafficking leads to glycogenic hepatopathy in zebrafish. [Abstract]

2024 Aug 14:dmm.050748. PMID: 39139065
Mol Brain

iTRAQ-based proteomic profiling reveals protein alterations after traumatic brain injury and supports thyroxine as a potential treatment. [Abstract]

2021 Jan 27;14(1):25. PMID: 33504361

L-Thyroxine sodium purchased from MedChemExpress. Usage Cited in: Mol Brain. 2021 Jan 27;14(1):25. [Abstract]

Effects of L-Thyroxine on histopathology post-TBI. a Brain sections stained with cresyl violet were shown on the left. Lesion volume was calculated from the sections.

L-Thyroxine sodium purchased from MedChemExpress. Usage Cited in: Mol Brain. 2021 Jan 27;14(1):25. [Abstract]

Brain water content of traumatized hemisphere increased 1 day post-TBI, whereas L-Thyroxine alleviates edema.

L-Thyroxine sodium purchased from MedChemExpress. Usage Cited in: Mol Brain. 2021 Jan 27;14(1):25. [Abstract]

BBB integrity was reflected by detecting the extravasation of EB dye (n = 3 per group).
Aalto University

2026.

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (78.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

0.5 M NaOH : 25 mg/mL (31.29 mM; ultrasonic and warming and adjust pH to 11 with NaOH and heat to 60°C)

H₂O : 14 mg/mL (17.53 mM; ultrasonic and adjust pH to 12 with NaOH)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2518 mL

6.2590 mL

12.5180 mL

5 mM

0.2504 mL

1.2518 mL

2.5036 mL

10 mM

0.1252 mL

0.6259 mL

1.2518 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (2.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.08 mg/mL (2.60 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Arici M, et al. Association between genetic polymorphism and levothyroxine bioavailability in hypothyroid patients. Endocr J. 2018 Mar 28;65(3):317-323. [Content Brief]

[2]. Corriveau S, et al. Levothyroxine treatment generates an abnormal uterine contractility patterns in an in vitro animalmodel. J Clin Transl Endocrinol. 2015 Sep 9;2(4):144-149. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / 0.5 M NaOH / DMSO	1 mM	1.2518 mL	6.2590 mL	12.5180 mL	31.2950 mL
	5 mM	0.2504 mL	1.2518 mL	2.5036 mL	6.2590 mL
	10 mM	0.1252 mL	0.6259 mL	1.2518 mL	3.1295 mL
	15 mM	0.0835 mL	0.4173 mL	0.8345 mL	2.0863 mL
0.5 M NaOH / DMSO	20 mM	0.0626 mL	0.3129 mL	0.6259 mL	1.5647 mL
	25 mM	0.0501 mL	0.2504 mL	0.5007 mL	1.2518 mL
	30 mM	0.0417 mL	0.2086 mL	0.4173 mL	1.0432 mL
DMSO	40 mM	0.0313 mL	0.1565 mL	0.3129 mL	0.7824 mL
	50 mM	0.0250 mL	0.1252 mL	0.2504 mL	0.6259 mL
	60 mM	0.0209 mL	0.1043 mL	0.2086 mL	0.5216 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

L-Thyroxine sodium

12 Cited Publications

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L-Thyroxine sodium

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Publications Citing Use of MedChemExpress (MCE) L-Thyroxine sodium

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (12)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

L-Thyroxine sodium Related Classifications

HY-18341B Related Classifications