1. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (995):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0218
    Thioflavin T 2390-54-7 ≥98.0%
    Thioflavin T is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections.
    Thioflavin T
  • HY-D0086
    DIDS sodium salt 67483-13-0 ≥99.0%
    DIDS sodium salt (MDL101114ZA) is a dual ABCA1 and VDAC1 inhibitor.
    DIDS sodium salt
  • HY-103311
    Ruthenium red 11103-72-3 ≥98.0%
    Ruthenium red (Ammoniated ruthenium oxychloride) is a polycationic dye widely used for electron microscopy (EM) of cells, tissues and vegetative bacteria. Ruthenium red strongly reacts with phospholipids and fatty acids and binds to acidic mucopolysaccharides. Ruthenium red is a L-type calcium current (ICa) blocker.
    Ruthenium red
  • HY-15096
    MKT-077 147366-41-4 ≥98.0%
    MKT-077 is a rhodacyanine dye and also a heat shock protein 70 (Hsp70) inhibitor which exhibits significant antitumor activity.
  • HY-B0324A
    Crystal Violet 548-62-9
    Crystal violet (Basic Violet 3) is a triarylmethane dye. Crystal Violet (Gentian Violet) has antiviral effects against H1N1 and also has prominent bactericidal activities.
    Crystal Violet
  • HY-136976
    WST-1 150849-52-8 ≥98.0%
    WST-1 is a Tetrazolium dye, widely used in the cell viability assays. WST-1 produces a water-soluble formazan product (WST-1 formazan) that occurs at the cell surface in NADPH dependent manner in viable cells.
  • HY-111496
    Sulfo-NHS-SS-Biotin sodium 325143-98-4
    Sulfo-NHS-SS-biotin is a long-chain cleavable and cell-impermeant amine-reactive biotinylation reagent. Sulfo-NHS-SS-biotin can be used for the labeling and purifying of cell-surface protein.
    Sulfo-NHS-SS-Biotin sodium
  • HY-D0987
    Stains-All 7423-31-6 99.91%
    Stains-All, a cationic carbocyanine dye, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP).
  • HY-19204
    Zinc phthalocyanine 14320-04-8 ≥98.0%
    Zinc phthalocyanine is commonly applied in industry (catalysts, photoconductors) and biomedical (photodynamic therapy, PDT). Zinc phthalocyanine can be used to photooxidise cyclohexane.
    Zinc phthalocyanine
  • HY-D0915
    Brilliant Blue FCF 3844-45-9 ≥98.0%
    Brilliant Blue FCF has the appearance of a reddish-blue powder.
    Brilliant Blue FCF
  • HY-B1539A
    Fuchsine base monohydrochloride 632-99-5 ≥99.0%
    Fuchsine base (monohydrochloride) is a magenta dye, which is certified for use for acid-fast staining with carbol-fuchsin.
    Fuchsine base monohydrochloride
  • HY-D0965
    Procion Blue HB 12236-82-7
    Procion Blue HB (Reactive Blue 2) is a purinergic antagonist.
    Procion Blue HB
  • HY-D0939
    Direct Blue 1 2610-05-1
    Direct Blue 1 (Chicago Sky Blue 6B) is a counterstain for background autofluorescence in immunofluorescence histochemistry. Direct Blue 1, structurally related to glutamate, is a potent and competitive VGLUT inhibitor without affecting plasma membrane transporters. Direct Blue 1 is the first small molecule PrP ligand capable of inhibiting Aβ binding.
    Direct Blue 1
  • HY-D0914
    Fast Green FCF 2353-45-9
    Fast Green FCF is a sea green triarylmethane food dye.
    Fast Green FCF
  • HY-D0256
    Direct Black 38 1937-37-7
    Direct Black 38 (Chlorazol Black E) is an azo dye. Direct Black 38 induces unscheduled DNA synthesis in liver and micronucleus in bone marrow of rats in vivo.
    Direct Black 38
  • HY-D0236
    Congo Red 573-58-0
    Congo Red is an azo dye. Congo Red (CR) binding been used as a diagnostic test for the presence of amyloid in tissue sections.
    Congo Red
  • HY-D0974
    Sulforhodamine B sodium salt 3520-42-1 98.88%
    Sulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.
    Sulforhodamine B sodium salt
  • HY-12489
    Ponceau S 6226-79-5 ≥98.0%
    Ponceau S (Acid Red 112) is the most commonly used stain for Western blotting. Ponceau S is applied as an acidic aqueous solution. Ponceau S is compatible with antibody-antigen binding, and stains the proteins on the membrane red.
    Ponceau S
  • HY-B0961
    Merbromin 129-16-8
    Merbromin acts as a topical antiseptic for minor cuts and scrapes and as a biological dye. Merbromin is a potent inhibitor against Zika virus (ZIKV) replication. Merbromin shows anti-ZIKV potency through ZIKVpro inhibition.
  • HY-D0042
    NBD-Cl 10199-89-0 ≥98.0%
    NBD-Cl is a nonfluorescent reagent which becomes highly fluorescent after reaction with thiol or amino groups.