1. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (326):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0218
    Thioflavin T 2390-54-7 98.72%
    Thioflavin T is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections.
    Thioflavin T
  • HY-D0086
    DIDS sodium salt 67483-13-0 >99.0%
    DIDS sodium salt (MDL101114ZA) is a dual ABCA1 and VDAC1 inhibitor.
    DIDS sodium salt
  • HY-D0915
    Brilliant Blue FCF 3844-45-9 99.10%
    Brilliant Blue FCF has the appearance of a reddish-blue powder.
    Brilliant Blue FCF
  • HY-B0324A
    Crystal Violet 548-62-9
    Crystal violet (Basic Violet 3) is a triarylmethane dye. Crystal Violet (Gentian Violet) has antiviral effects against H1N1 and also has prominent bactericidal activities.
    Crystal Violet
  • HY-D0965
    Procion Blue HB 12236-82-7
    Procion Blue HB (Reactive Blue 2) is a purinergic antagonist.
    Procion Blue HB
  • HY-B0961
    Merbromin 129-16-8
    Merbromin (Mercury dibromofluorescein disodium salt) is a xanthene dye.
  • HY-15096
    MKT-077 147366-41-4 >98.0%
    MKT-077 is a rhodacyanine dye and also a heat shock protein 70 (Hsp70) inhibitor which exhibits significant antitumor activity.
  • HY-D0236
    Congo Red 573-58-0
    Congo Red is an azo dye. Congo Red (CR) binding been used as a diagnostic test for the presence of amyloid in tissue sections.
    Congo Red
  • HY-D0042
    NBD-Cl 10199-89-0 >98.0%
    NBD-Cl is a nonfluorescent reagent which becomes highly fluorescent after reaction with thiol or amino groups.
  • HY-D0970
    Diphenyl Blue 72-57-1
    Diphenyl Blue (Direct Blue 14) is used as reference dye for group of azo dye.
    Diphenyl Blue
  • HY-D0914
    Fast Green FCF 2353-45-9
    Fast Green FCF is a sea green triarylmethane food dye.
    Fast Green FCF
  • HY-19204
    Zinc phthalocyanine 14320-04-8 >98.0%
    Zinc phthalocyanine is commonly applied in industry (catalysts, photoconductors) and biomedical (photodynamic therapy, PDT). Zinc phthalocyanine can be used to photooxidise cyclohexane.
    Zinc phthalocyanine
  • HY-D0024
    Sudan I 842-07-9 >98.0%
    Sudan I is an organic compound, typically classified as an azo dye.
    Sudan I
  • HY-D0974
    Sulforhodamine B sodium salt 3520-42-1
    Sulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.
    Sulforhodamine B sodium salt
  • HY-B1539A
    Fuchsine base monohydrochloride 632-99-5 100.00%
    Fuchsine base (monohydrochloride) is a magenta dye, which is certified for use for acid-fast staining with carbol-fuchsin.
    Fuchsine base monohydrochloride
  • HY-D0193
    Ponceau 4R 2611-82-7 99.80%
    Ponceau 4R is a synthetic colourant that may be used as a food colouring.
    Ponceau 4R
  • HY-D0169A
    Phenol Red sodium salt 34487-61-1
    Phenol Red sodium salt is a water soluble pH indicator used in the 6.8 (yellow) to 8.2 (red) range.
    Phenol Red sodium salt
  • HY-D0012
    Bromothymol Blue 76-59-5 >98.0%
    Bromothymol Blue is a pH indicator.
    Bromothymol Blue
  • HY-N0938
    Safflower yellow 1401-20-3
    Safflower yellow is extracted from the flowers of the plant safflower (Carthamus tinctorius) and as the traditional Chinese medicine it has been extensively used for the treatment of cardio cerebrovascular diseases.
    Safflower yellow
  • HY-D0249
    Sunset Yellow FCF 2783-94-0 >98.0%
    Sunset Yellow FCF (Orange Yellow S) is a petroleum-derived orange azo dye with a pH dependent maximum absorption at about 480 nm at pH 1 and 443 nm at pH 13.
    Sunset Yellow FCF