Sulforhodamine B sodium salt (Synonyms: Acid Red 52; Kiton Red 620)

Name: Sulforhodamine B sodium salt
Brand: MedChemExpress
SKU: HY-D0974
Rating: 5.0 (6 reviews)

Cat. No.: HY-D0974 Purity: 99.43%: Data Sheet
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Sulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.

For research use only. We do not sell to patients.

CAS No. : 3520-42-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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Based on 6 publication(s) in Google Scholar

Other Forms of Sulforhodamine B sodium salt:

Lissamine rhodamine B In-stock

6 Publications Citing Use of MCE Sulforhodamine B sodium salt

Cell Proliferation/Viability Assay

: Sulforhodamine B sodium salt purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Apr 17.; Analysis of cell viability (relative to control) in indicated ccRCC cell lines transfected with either non-targeting control siRNA (siCtrl) or pRb-targeting siRNA (sipRb), measured via a Sulforhodamine B sodium salt (SRB; 0.04% (wt/vol); 30 min ) assay.

: Sulforhodamine B sodium salt purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Apr 17.; Analysis of cell viability (relative to control) in VHL-expressing 786-O cells transfected with either non-targeting control siRNA (siCtrl) or pRb targeting siRNA (sipRb), measured via a Sulforhodamine B sodium salt (SRB; 0.04% (wt/vol); 30 min ) assay.

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Description

Sulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.

In Vitro

Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

580.65

Formula

C₂₇H₂₉N₂NaO₇S₂

CAS No.

3520-42-1

Appearance

Solid

Color

Brown to reddish brown

Emission (Em)

585

Excitation (Ex)

565

SMILES

O=S(C1=CC=C(C2=C3C=CC(N(CC)CC)=CC3=[O+]C4=C2C=CC(N(CC)CC)=C4)C(S(=O)([O-])=O)=C1)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (107.64 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (86.11 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7222 mL	8.6110 mL	17.2221 mL
5 mM	0.3444 mL	1.7222 mL	3.4444 mL
10 mM	0.1722 mL	0.8611 mL	1.7222 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.43%

Select Batch:

Data Sheet (283 KB) SDS (393 KB)

COA (259 KB) HNMR (270 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984. [Content Brief]

Cell Assay
^[1]

Gently add 25 μL (96-well format) or 5 μL (384-well format) cold 50% (wt/vol) TCA to each well directly to medium supernatant, and incubate the plates at 4 °C for 1 h. Mixing is not required, as this could lead to some cells detaching from the bottom of the well. Wash the plates four times by submerging the plate in a tub with slow-running tap water and remove excess water by gently tapping the plate into a paper towel. After the last wash allow the plate to air-dry at room temperature. Add 50 μL (96-well format) or 20 μL (384-well format) of 0.04% (wt/vol) SRB solution to each well. Leave at room temperature for 1 h and then quickly rinse the plates four times with 1% (vol/vol) acetic acid (200 μL for 96-well format or 30 μL for 384-well format) to remove unbound dye. Allow the plate to air-dry at room temperature^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.7222 mL	8.6110 mL	17.2221 mL	43.0552 mL
	5 mM	0.3444 mL	1.7222 mL	3.4444 mL	8.6110 mL
	10 mM	0.1722 mL	0.8611 mL	1.7222 mL	4.3055 mL
	15 mM	0.1148 mL	0.5741 mL	1.1481 mL	2.8703 mL
	20 mM	0.0861 mL	0.4306 mL	0.8611 mL	2.1528 mL
	25 mM	0.0689 mL	0.3444 mL	0.6889 mL	1.7222 mL
	30 mM	0.0574 mL	0.2870 mL	0.5741 mL	1.4352 mL
	40 mM	0.0431 mL	0.2153 mL	0.4306 mL	1.0764 mL
	50 mM	0.0344 mL	0.1722 mL	0.3444 mL	0.8611 mL
	60 mM	0.0287 mL	0.1435 mL	0.2870 mL	0.7176 mL
	80 mM	0.0215 mL	0.1076 mL	0.2153 mL	0.5382 mL
DMSO	100 mM	0.0172 mL	0.0861 mL	0.1722 mL	0.4306 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sulforhodamine B3520-42-1Acid Red 52 Kiton Red 620Acid Red52Acid Red-52Kiton Red620Kiton Red 620Kiton Red-620Fluorescent DyeEnvironmental PollutantsEnvironmental ContaminantsInhibitorinhibitorinhibit

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Sulforhodamine B sodium salt (Synonyms: Acid Red 52; Kiton Red 620)

Customer Review

Other Forms of Sulforhodamine B sodium salt:

Sulforhodamine B sodium salt Related Antibodies

6 Publications Citing Use of MCE Sulforhodamine B sodium salt

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Sulforhodamine B sodium salt Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Sulforhodamine B sodium salt Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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