Sulforhodamine B sodium salt
Based on 6 publication(s) in Google Scholar
Sulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.
For research use only. We do not sell to patients.
- Purity: 99.43%
- CAS No.: 3520-42-1
- Formula: C27H29N2NaO7S2
- Molecular Weight:580.65
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Sulforhodamine B sodium salt
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
Biological Activity
Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 3520-42-1
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Appearance Solid
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Molecular Weight 580.65
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Formula C27H29N2NaO7S2
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Color Brown to reddish brown
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SMILES
O=S(C1=CC=C(C2=C3C=CC(N(CC)CC)=CC3=[O+]C4=C2C=CC(N(CC)CC)=C4)C(S(=O)([O-])=O)=C1)(O[Na])=O
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Synonyms
Acid Red 52; Kiton Red 620
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (6)
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Journal Impact Factor
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Most Recent
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Adv Mater
Crosstalk-Free, High-Resolution Pressure Sensor Arrays Enabled by High-Throughput Laser Manufacturing. [Abstract]2022 May;34(21):e2200517. PMID: 35332964 -
J Agric Food Chem
Exogenous Phytohormone Activators Enhance Rice Resistance to Brown Planthoppers by Boosting the Phenylpropanoid Pathway under Elevated CO2. [Abstract]2026 Mar 18;74(10):8368-8379. PMID: 41781190 -
Front Pharmacol
Selective anti-cancer effects of cannabidiol and Δ9-tetrahydrocannabinol via PI3K/AKT/mTOR inhibition and PTEN restoration in ovarian cancer cells. [Abstract]2025 Dec 15:16:1693129. PMID: 41472794 -
Mol Pharm
Lipids Extracted from Mycobacterial Membrane and Enveloped PLGA Nanoparticles for Encapsulating Antibacterial Drugs Elicit Synergistic Antimicrobial Response against Mycobacteria. [Abstract]2024 May 6;21(5):2238-2249. PMID: 38622497 -
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Sulforhodamine B sodium salt purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Apr 17.
Analysis of cell viability (relative to control) in indicated ccRCC cell lines transfected with either non-targeting control siRNA (siCtrl) or pRb-targeting siRNA (sipRb), measured via a Sulforhodamine B sodium salt (SRB; 0.04% (wt/vol); 30 min ) assay.
Sulforhodamine B sodium salt purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Apr 17.
Analysis of cell viability (relative to control) in VHL-expressing 786-O cells transfected with either non-targeting control siRNA (siCtrl) or pRb targeting siRNA (sipRb), measured via a Sulforhodamine B sodium salt (SRB; 0.04% (wt/vol); 30 min ) assay.
Solvent & Solubility
DMSO : 62.5 mg/mL (107.64 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (86.11 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Gently add 25 μL (96-well format) or 5 μL (384-well format) cold 50% (wt/vol) TCA to each well directly to medium supernatant, and incubate the plates at 4 °C for 1 h. Mixing is not required, as this could lead to some cells detaching from the bottom of the well. Wash the plates four times by submerging the plate in a tub with slow-running tap water and remove excess water by gently tapping the plate into a paper towel. After the last wash allow the plate to air-dry at room temperature. Add 50 μL (96-well format) or 20 μL (384-well format) of 0.04% (wt/vol) SRB solution to each well. Leave at room temperature for 1 h and then quickly rinse the plates four times with 1% (vol/vol) acetic acid (200 μL for 96-well format or 30 μL for 384-well format) to remove unbound dye. Allow the plate to air-dry at room temperature[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 1.7222 mL | 8.6110 mL | 17.2221 mL | 43.0552 mL |
| 5 mM | 0.3444 mL | 1.7222 mL | 3.4444 mL | 8.6110 mL | |
| 10 mM | 0.1722 mL | 0.8611 mL | 1.7222 mL | 4.3055 mL | |
| 15 mM | 0.1148 mL | 0.5741 mL | 1.1481 mL | 2.8703 mL | |
| 20 mM | 0.0861 mL | 0.4306 mL | 0.8611 mL | 2.1528 mL | |
| 25 mM | 0.0689 mL | 0.3444 mL | 0.6889 mL | 1.7222 mL | |
| 30 mM | 0.0574 mL | 0.2870 mL | 0.5741 mL | 1.4352 mL | |
| 40 mM | 0.0431 mL | 0.2153 mL | 0.4306 mL | 1.0764 mL | |
| 50 mM | 0.0344 mL | 0.1722 mL | 0.3444 mL | 0.8611 mL | |
| 60 mM | 0.0287 mL | 0.1435 mL | 0.2870 mL | 0.7176 mL | |
| 80 mM | 0.0215 mL | 0.1076 mL | 0.2153 mL | 0.5382 mL | |
| DMSO | 100 mM | 0.0172 mL | 0.0861 mL | 0.1722 mL | 0.4306 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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