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  4. Lissamine rhodamine B

Lissamine rhodamine B is a red-fluorescent dye, it is a derivative of rhodamine. Lissamine rhodamine B can be used as a fluorescent probe to develop competitive aptamer fluorescence anisotropy/polarization (FA/FP) assays.

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CAS No. : 2609-88-3

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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of Lissamine rhodamine B:

Lissamine rhodamine B Related Antibodies

Top Publications Citing Use of Products

    Lissamine rhodamine B purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Apr 17.

    Analysis of cell viability (relative to control) in indicated ccRCC cell lines transfected with either non-targeting control siRNA (siCtrl) or pRb-targeting siRNA (sipRb), measured via a Sulforhodamine B sodium salt (SRB; 0.04% (wt/vol); 30 min ) assay.

    Lissamine rhodamine B purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Apr 17.

    Analysis of cell viability (relative to control) in VHL-expressing 786-O cells transfected with either non-targeting control siRNA (siCtrl) or pRb targeting siRNA (sipRb), measured via a Sulforhodamine B sodium salt (SRB; 0.04% (wt/vol); 30 min ) assay.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Lissamine rhodamine B is a red-fluorescent dye, it is a derivative of rhodamine. Lissamine rhodamine B can be used as a fluorescent probe to develop competitive aptamer fluorescence anisotropy/polarization (FA/FP) assays[1][2].

    In Vitro

    Lissamine rhodamine B (1 mg; 1 h; room temperature; dark) can be used to detect ochratoxin A (OTA) which is a common mycotoxin[1].
    Lissamine rhodamine B have fluorescence properties, with the fluorescence detection conditions: excitation wavelength 555 nm, fluorescence emission spectrum detection 580 nm[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Lissamine rhodamine B Related Antibodies
    In Vivo

    Lissamine rhodamine B can be used as an aradiotracer for imaging tumor mice by positron emission tomography (PET)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    558.67

    Formula

    C27H30N2O7S2
    CAS No.
    Appearance

    Solid

    SMILES

    O=S(C1=CC=C(C2=C3C=CC(N(CC)CC)=CC3=[O+]C4=C2C=CC(N(CC)CC)=C4)C(S(=O)([O-])=O)=C1)(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 5 mg/mL (8.95 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7900 mL 8.9498 mL 17.8997 mL
    5 mM 0.3580 mL 1.7900 mL 3.5799 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.00%

    SDS (558 KB)

    COA (257 KB) HNMR (271 KB) LCMS (87 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.7900 mL 8.9498 mL 17.8997 mL 44.7491 mL
    5 mM 0.3580 mL 1.7900 mL 3.5799 mL 8.9498 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Lissamine rhodamine B Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Lissamine rhodamine B2609-88-3Fluorescent Dyeredrhodamineprobefluorescent dyeFAFPPETInhibitorinhibitorinhibit

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