1. Others Anti-infection
  2. Fluorescent Dye Influenza Virus Bacterial
  3. Crystal Violet

Crystal Violet  (Synonyms: Basic Violet 3; Gentian Violet; Methyl Violet 10B)

Cat. No.: HY-B0324A Purity: 95.03%
COA Handling Instructions

Crystal Violet, also known as Gentian violet, methyl violet 10B, is a triphenyl-methane, an alkaline dye that binds to DNA in the nucleus of a cell, staining it a deep purple. It is often used for Gram staining to classify bacteria, or for cell or histological staining[1].

For research use only. We do not sell to patients.

Crystal Violet Chemical Structure

Crystal Violet Chemical Structure

CAS No. : 548-62-9

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
500 mg USD 55 In-stock
5 g USD 61 In-stock
10 g USD 66 In-stock
50 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 32 publication(s) in Google Scholar

Other Forms of Crystal Violet:

Top Publications Citing Use of Products

32 Publications Citing Use of MCE Crystal Violet

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Crystal Violet, also known as Gentian violet, methyl violet 10B, is a triphenyl-methane, an alkaline dye that binds to DNA in the nucleus of a cell, staining it a deep purple. It is often used for Gram staining to classify bacteria, or for cell or histological staining[1].

In Vitro

General Protocol

1.Preparation of MitoSOX Red working solution
Dilute the 2 g Crystal Violet in 20 mL 95% ethanol andmixed with 80 ml 1% ammonium oxalate solution solution for 24 h to obtain working solution.
2.Sample processing
2.1 Paraffin section:
First dewaxing with xylene for 5-10 min, then with fresh xylene, then dewaxing with 100% ethanol for 5 min after dewaxing for 5-10 min. Then 90% ethanol was used for 2 min. At last, 70% ethanol was used for 2 min. DDH2O 2 min.
2.2 Frozen section:
distilled water for 2 min.
2.3 Tissue culture cells:
fixed with 4% paraformaldehyde for more than 10 min. Wash with distilled water for 2 min, replace with fresh distilled water, and wash again for 2 min.
Note: Other fixatives can be selected according to specific experiments.
3.Crystal violet staining
3.1 After the sample to be tested is treated with the above method, the crystal violet dyeing solution is directly added and dyed for 10 minutes at room temperature (the dyeing solution needs to cover the sample, and the specific dyeing time can be adjusted according to the required dyeing results).
3.2 Wash well in distilled water.
3.3 Dry at room temperature.
3.4 Observe and photograph by light microscope.

Storage
4°C, 2 year
Protect from light

Precautions
1. It is recommended to take 1-2 samples for pre-test when using this product for the first time.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
Molecular Weight

407.98

Formula

C25H30ClN3

CAS No.
Appearance

Solid

Color

Light green to green

SMILES

C/[N+](C)=C1C=C/C(C=C/1)=C(C2=CC=C(N(C)C)C=C2)\C3=CC=C(N(C)C)C=C3.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 1 years; -20°C, 6 months (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (245.11 mM)

H2O : 5 mg/mL (12.26 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4511 mL 12.2555 mL 24.5110 mL
5 mM 0.4902 mL 2.4511 mL 4.9022 mL
10 mM 0.2451 mL 1.2256 mL 2.4511 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.13 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.13 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.13 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation
Dyeing Example
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Crystal Violet Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Crystal Violet
Cat. No.:
HY-B0324A
Quantity:
MCE Japan Authorized Agent: