Sulfo-NHS-SS-Biotin sodium

Name: Sulfo-NHS-SS-Biotin sodium
Brand: MedChemExpress
SKU: HY-111496
Rating: 5.0 (4 reviews)

Cat. No.: HY-111496 Purity: 97.0%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Sulfo-NHS-SS-biotin sodium is a long-chain cleavable and cell-impermeant amine-reactive biotinylation reagent. Sulfo-NHS-SS-biotin sodium can be used for the labeling and purifying of cell-surface protein.

For research use only. We do not sell to patients.

CAS No. : 325143-98-4

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 4 publication(s) in Google Scholar

4 Publications Citing Use of MCE Sulfo-NHS-SS-Biotin sodium

: Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 May 31:1-21. [Abstract]; 293T cells were treated with 1 mL PBS-CM supplemented with Sulfo-NHS-SS-biotin (0.5 mg/mL; 30 min; 40 °C) with gentle agitation. Immunoblot analysis of cell surface-biotinylated ZDHHC5 was performed.

: Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Sep 29;26(19):9522.; HT-22 cells were incubated in Hank's balanced salt solution containing Sulfo-NHS-SS-biotin (1 mg/mL; 30 min; 40 ℃) to biotinylate membrane proteins. Expression level of biotinylated GFRα1 following 24 h exposure to 2 mM METH was measured.

: Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Toxicology. 2025 Jan 10:154047. [Abstract]; Hippocampal slices were biotinylated in Hanks’ balanced salt solution containing Sulfo-NHS-SS-biotin (1 mg/mL; 30 min; 4℃). Levels of biotinylated TrkB were measured after incubation with METH (2 mM) for 24 h.

: Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Toxicology. 2025 Jan 10:154047. [Abstract]; HT-22 cells were biotinylated in Hanks' balanced salt solution containing Sulfo-NHS-SS-biotin (1 mg/mL; 30 min; 4°C), then rinsed three times with HBSS. Biotinylated TrkB expression levels in HT-22 cells were measured after incubation with METH (2 mM) for 24 h.

: Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Mar 12;57(3):495-512.e11. [Abstract]; BV2 cells were collected and were labeled with Sulfo-NHS-SS-Biotin sodium (1 mg/mL; 1 h; 4 ℃;). Membrane proteins extracted were analyzed by Western blotting.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: cell-surface protein^[1]

In Vitro

Sulfo-NHS-SS-Biotin sodium is a cell-surface-labeling reagent, it is a negatively charged reagent and does not permeate cell membranes^[1].
Sulfo-NHS-SS-Biotin sodium reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides^[1].
Sulfo-NHS-SS-Biotin sodium has a sulfonate group and prevents it from permeating cell membranes. Thr group’s cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns^[1].
Sulfo-NHS-SS-Biotin sodium (1 mg/mL; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS).
The biotinylation reactions are terminated with 100 mM glycine in PBS.
After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail (HY-K0010).
Biotinylated membrane proteins are precipitated with streptavidin-sepharose and proteins are eluted with SDS sample buffer^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

606.69

Formula

C₁₉H₂₇N₄NaO₉S₄

CAS No.

325143-98-4

Appearance

Solid

Color

White to yellow

SMILES

O=C(ON1C(C(S(=O)(O[Na])=O)CC1=O)=O)CCSSCCNC(CCCC[C@@H]2SC[C@]([C@]2([H])N3)([H])NC3=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (164.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6483 mL	8.2414 mL	16.4829 mL
5 mM	0.3297 mL	1.6483 mL	3.2966 mL
10 mM	0.1648 mL	0.8241 mL	1.6483 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 97.0%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (245 KB) HNMR (300 KB) MS (231 KB)

Handling Instructions (2659 KB)

References

[1]. Chia CP,et al. Phagocytosis in Dictyostelium discoideum is inhibited by antibodies directed primarily against common carbohydrate epitopes of a major cell-surface plasma membrane glycoprotein.Exp Cell Res. 1989 Mar;181(1):11-26. [Content Brief]

[2]. Jo M, et al. Cell signaling by urokinase-type plasminogen activator receptor induces stem cell-like properties in breast cancer cells. Cancer Res. 2010 Nov 1;70(21):8948-58. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6483 mL	8.2414 mL	16.4829 mL	41.2072 mL
	5 mM	0.3297 mL	1.6483 mL	3.2966 mL	8.2414 mL
	10 mM	0.1648 mL	0.8241 mL	1.6483 mL	4.1207 mL
	15 mM	0.1099 mL	0.5494 mL	1.0989 mL	2.7471 mL
	20 mM	0.0824 mL	0.4121 mL	0.8241 mL	2.0604 mL
	25 mM	0.0659 mL	0.3297 mL	0.6593 mL	1.6483 mL
	30 mM	0.0549 mL	0.2747 mL	0.5494 mL	1.3736 mL
	40 mM	0.0412 mL	0.2060 mL	0.4121 mL	1.0302 mL
	50 mM	0.0330 mL	0.1648 mL	0.3297 mL	0.8241 mL
	60 mM	0.0275 mL	0.1374 mL	0.2747 mL	0.6868 mL
	80 mM	0.0206 mL	0.1030 mL	0.2060 mL	0.5151 mL
	100 mM	0.0165 mL	0.0824 mL	0.1648 mL	0.4121 mL