Sulfo-NHS-SS-Biotin sodium
Based on 7 publication(s) in Google Scholar
Sulfo-NHS-SS-biotin sodium is a long-chain cleavable and cell-impermeant amine-reactive biotinylation reagent. Sulfo-NHS-SS-biotin sodium can be used for the labeling and purifying of cell-surface protein.
For research use only. We do not sell to patients.
- Purity: 97.0%
- CAS No.: 325143-98-4
- Formula: C19H27N4NaO9S4
- Molecular Weight:606.69
-
Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
HY-K0208
Streptavidin Magnetic Beads
-
HY-140129
NHS-SS-biotin
-
HY-100215
Biotin Hydrazide
-
HY-136769
Biotin-HPDP
-
HY-34477
2-Iodoacetamide
-
HY-D0187
L-Glutathione reduced
-
HY-100558
Bafilomycin A1
-
HY-D0799
Sulfo-NHS-LC-Biotin sodium
-
HY-13679
Sodium 2-mercaptoethanesulfonate
-
HY-140128
DBCO-S-S-PEG3-biotin
-
HY-Y0966
Glycine
-
HY-B0496
PMSF
Publications Citing Use of MedChemExpress (MCE) Sulfo-NHS-SS-Biotin sodium
More- Immunity. 2024 Mar 12;57(3):495-512.e11. [Abstract]
- Autophagy. 2025 May 31:1-21. [Abstract]
- Mol Ther. 2026 Jul 1;34(7):4212-4225. [Abstract]
- Int J Mol Sci. 2025 Sep 29;26(19):9522. [Abstract]
- Toxicology. 2025 Feb:511:154047. [Abstract]
- Sci Rep. 2026 Apr 16;16(1):17626. [Abstract]
- Reprod Toxicol. 2026 Jun 4:144:109271. [Abstract]
Customer Validation & Images
-
WB
-
WB
-
WB
-
WB
-
WB
Biological Activity
IC50: cell-surface protein[1]
Sulfo-NHS-SS-Biotin sodium is a cell-surface-labeling reagent, it is a negatively charged reagent and does not permeate cell membranes[1]. Sulfo-NHS-SS-Biotin sodium reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides[1]. Sulfo-NHS-SS-Biotin sodium has a sulfonate group and prevents it from permeating cell membranes. Thr group’s cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns[1]. Sulfo-NHS-SS-Biotin sodium (1 mg/mL; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS). The biotinylation reactions are terminated with 100 mM glycine in PBS. After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail (HY-K0010). Biotinylated membrane proteins are precipitated with streptavidin-sepharose and proteins are eluted with SDS sample buffer[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 325143-98-4
-
Appearance Solid
-
Molecular Weight 606.69
-
Formula C19H27N4NaO9S4
-
Color White to yellow
-
SMILES
O=C(ON1C(C(S(=O)(O[Na])=O)CC1=O)=O)CCSSCCNC(CCCC[C@@H]2SC[C@]([C@]2([H])N3)([H])NC3=O)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (7)
-
Journal Impact Factor
-
Most Recent
-
Immunity
Disruption of the Na+/K+-ATPase-purinergic P2X7 receptor complex in microglia promotes stress-induced anxiety. [Abstract]2024 Mar 12;57(3):495-512.e11. PMID: 38395698
Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Mar 12;57(3):495-512.e11. [Abstract]
BV2 cells were collected and were labeled with Sulfo-NHS-SS-Biotin sodium (1 mg/mL; 1 h; 4 ℃;). Membrane proteins extracted were analyzed by Western blotting.
-
Autophagy
2025 May 31:1-21. PMID: 40394978
Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 May 31:1-21. [Abstract]
293T cells were treated with 1 mL PBS-CM supplemented with Sulfo-NHS-SS-Biotin sodium (0.5 mg/mL; 30 min; 40 °C) with gentle agitation. Immunoblot analysis of cell surface-biotinylated ZDHHC5 was performed.
-
Mol Ther
2026 Jul 1;34(7):4212-4225. PMID: 41925277 -
Int J Mol Sci
2025 Sep 29;26(19):9522. PMID: 41096789
Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Sep 29;26(19):9522. [Abstract]
HT-22 cells were incubated in Hank's balanced salt solution containing Sulfo-NHS-SS-Biotin sodium (1 mg/mL; 30 min; 40 ℃) to biotinylate membrane proteins. Expression level of biotinylated GFRα1 following 24 h exposure to 2 mM METH was measured.
-
Toxicology
Methamphetamine inhibits huntingtin-associated protein 1-mediated tyrosine receptor kinase B endocytosis resulting the neuroprotective dysfunction of brain-derived neurotrophic factor. [Abstract]2025 Feb:511:154047. PMID: 39800156
Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Toxicology. 2025 Feb:511:154047. [Abstract]
Total TrkB, pTrkB (Tyr516), membrane TrkB, biotinylated TrkB, and HAP1 expression levels in HT-22 cells after incubation with METH (2 mM) for 24 h. HT-22 cells were first biotinylated in Hanks’ balanced salt solution (HBSS) containing 1 mg/mL Sulfo-NHS-SS-biotin sodium for 30 min at 4 °C, then rinsed three times with HBSS.
Sulfo-NHS-SS-Biotin sodium purchased from MedChemExpress. Usage Cited in: Toxicology. 2025 Feb:511:154047. [Abstract]
Levels of total TrkB, pTrkB (Tyr516), biotinylated TrkB, BDNF, and apoptosis-related proteins in hippocampal slices after incubation with METH (2 mM) for 24 h. Hippocampal slices were first biotinylated in Hanks’ balanced salt solution (HBSS) containing 1 mg/mL Sulfo-NHS-SS-biotin sodium for 30 min at 4 °C, then rinsed three times with HBSS.
-
Sci Rep
Galectin-4 contributes to the maintenance of expression and activation of multiple receptor-type kinases involved in peritoneal metastasis. [Abstract]2026 Apr 16;16(1):17626. PMID: 41991671 -
Reprod Toxicol
Silica nanoparticles disrupt blood-testis barrier integrity in Sertoli cells by inducing inflammation-mediated p38 MAPK pathway. [Abstract]2026 Jun 4:144:109271. PMID: 42248310
Solvent & Solubility
DMSO : 100 mg/mL (164.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (271 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Chia CP,et al. Phagocytosis in Dictyostelium discoideum is inhibited by antibodies directed primarily against common carbohydrate epitopes of a major cell-surface plasma membrane glycoprotein.Exp Cell Res. 1989 Mar;181(1):11-26. [Content Brief]
[2]. Jo M, et al. Cell signaling by urokinase-type plasminogen activator receptor induces stem cell-like properties in breast cancer cells. Cancer Res. 2010 Nov 1;70(21):8948-58. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6483 mL | 8.2414 mL | 16.4829 mL | 41.2072 mL |
| 5 mM | 0.3297 mL | 1.6483 mL | 3.2966 mL | 8.2414 mL | |
| 10 mM | 0.1648 mL | 0.8241 mL | 1.6483 mL | 4.1207 mL | |
| 15 mM | 0.1099 mL | 0.5494 mL | 1.0989 mL | 2.7471 mL | |
| 20 mM | 0.0824 mL | 0.4121 mL | 0.8241 mL | 2.0604 mL | |
| 25 mM | 0.0659 mL | 0.3297 mL | 0.6593 mL | 1.6483 mL | |
| 30 mM | 0.0549 mL | 0.2747 mL | 0.5494 mL | 1.3736 mL | |
| 40 mM | 0.0412 mL | 0.2060 mL | 0.4121 mL | 1.0302 mL | |
| 50 mM | 0.0330 mL | 0.1648 mL | 0.3297 mL | 0.8241 mL | |
| 60 mM | 0.0275 mL | 0.1374 mL | 0.2747 mL | 0.6868 mL | |
| 80 mM | 0.0206 mL | 0.1030 mL | 0.2060 mL | 0.5151 mL | |
| 100 mM | 0.0165 mL | 0.0824 mL | 0.1648 mL | 0.4121 mL |
Sulfo-NHS-SS-Biotin sodium Related Classifications
HY-111496 Related Classifications
Powered by Bioz