HADA hydrochloride (Synonyms: HCC-Amino-D-alanine hydrochloride)

Name: HADA hydrochloride
Brand: MedChemExpress
SKU: HY-131045
Rating: 4.5 (1 reviews)

Cat. No.: HY-131045 Purity: 99.08%: FAQs
Data Sheet SDS COA Handling Instructions

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HADA hydrochloride (HCC-Amino-D-alanine hydrochloride) is a blue (λ_em~450 nm) fluorescent D-amino acid (FDAA). FDAAs are efficiently incorporated into the peptidoglycans (PGs) of diverse bacterial species at the sites of PG biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation.

For research use only. We do not sell to patients.

HADA hydrochloride Chemical Structure

CAS No. : 2253733-10-5

Size	Price	Stock	Quantity
5 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 950	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1450	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2250	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE HADA hydrochloride

•bioRxiv. 2023 Feb 20.

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

FDAA labeling can take as little as 30s for a rapidly growing species such as Escherichia coli. Although HADA hydrochloride is dimmer and less photostable than FDL or TDL, it most reproducibly and robustly labels the PG of most bacterial species, typically without the need for extensive optimization^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

328.71

Formula

C₁₃H₁₃ClN₂O₆

CAS No.

2253733-10-5

Appearance

Solid

Color

White to off-white

SMILES

N[C@H](CNC(C1=CC2=CC=C(O)C=C2OC1=O)=O)C(O)=O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (380.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0422 mL	15.2110 mL	30.4220 mL
5 mM	0.6084 mL	3.0422 mL	6.0844 mL
10 mM	0.3042 mL	1.5211 mL	3.0422 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (6.33 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.08%

Select Batch:

Data Sheet (272 KB) SDS (0 KB)

COA (247 KB) HNMR (208 KB) LCMS (111 KB)

Handling Instructions (2659 KB)

References

[1]. Erkin Kuru, et al. Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ. Nat Protoc. 2015 Jan;10(1):33-52. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0422 mL	15.2110 mL	30.4220 mL	76.0549 mL
	5 mM	0.6084 mL	3.0422 mL	6.0844 mL	15.2110 mL
	10 mM	0.3042 mL	1.5211 mL	3.0422 mL	7.6055 mL
	15 mM	0.2028 mL	1.0141 mL	2.0281 mL	5.0703 mL
	20 mM	0.1521 mL	0.7605 mL	1.5211 mL	3.8027 mL
	25 mM	0.1217 mL	0.6084 mL	1.2169 mL	3.0422 mL
	30 mM	0.1014 mL	0.5070 mL	1.0141 mL	2.5352 mL
	40 mM	0.0761 mL	0.3803 mL	0.7605 mL	1.9014 mL
	50 mM	0.0608 mL	0.3042 mL	0.6084 mL	1.5211 mL
	60 mM	0.0507 mL	0.2535 mL	0.5070 mL	1.2676 mL
	80 mM	0.0380 mL	0.1901 mL	0.3803 mL	0.9507 mL
	100 mM	0.0304 mL	0.1521 mL	0.3042 mL	0.7605 mL

HADA hydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HADA2253733-10-5HCC-Amino-D-alanineBacterialFDAAspeptidoglycansprobingbacterialsituInhibitorinhibitorinhibit

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HADA hydrochloride (Synonyms: HCC-Amino-D-alanine hydrochloride)

Customer Review

HADA hydrochloride Related Antibodies

1 Publications Citing Use of MCE HADA hydrochloride

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

HADA hydrochloride Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

HADA hydrochloride Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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