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  3. Bacterial
  4. HADA hydrochloride

HADA hydrochloride  (Synonyms: HCC-Amino-D-alanine hydrochloride)

Cat. No.: HY-131045 Purity: 98.01%
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HADA hydrochloride (HCC-Amino-D-alanine hydrochloride) is a blue (λem~450 nm) fluorescent D-amino acid (FDAA). FDAAs are efficiently incorporated into the peptidoglycans (PGs) of diverse bacterial species at the sites of PG biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation.

For research use only. We do not sell to patients.

CAS No. : 2253733-10-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of HADA hydrochloride:

HADA hydrochloride Related Antibodies

Top Publications Citing Use of Products

    HADA hydrochloride purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2025 Jul;14(7):e70131.  [Abstract]

    Cells were labelled with FM4-64 (1 µM, HY-103466), and HADA hydrochloride (20 µM), followed by incubation in the dark for 30 min. Fluorescence microscopy analysis indicated enhanced peptidoglycan integrity in Δessd‐1, Δrrrd, Δrzod, Δrrrq and Δessd‐2 mutants. Scale bar, 10 µm.

    HADA hydrochloride purchased from MedChemExpress. Usage Cited in: Microbiol Res. 2025 Sep 24:302:128350.

    Fluorescence microscopy of AR-GBS labeled with HADA hydrochloride (250 μM; 30 min) following treatment with 20 mM PAG plus 1/4 MIC AMP.

    HADA hydrochloride purchased from MedChemExpress. Usage Cited in: Pharmaceuticals (Basel). 2025 Sep 28;18(10):1458.

    The impact of BA in combination with KM on bacterial peptidoglycan synthesis was tracked using HADA hydrochloride (250 μM; 30 min). Peptidoglycan synthesis in MRSA USA300 and Staphylococcus aureus ATCC29213, observed via confocal laser scanning microscopy (CLSM). Scale bar = 5 μm. The regions of interest are marked by white circles, where a decrease in fluorescence intensity and abnormal morphology of some cells can be observed.

    HADA hydrochloride purchased from MedChemExpress. Usage Cited in: mBio. 2025 Apr 9;16(4):e0297524.  [Abstract]

    Ampicillin affects the incorporation of newly synthesized cell wall in PG recycling mutants. HADA labeling of strains CB15N, RP47 (∆sdpA), and RP56 (∆ampG) in the presence (+ amp) and absence (- amp) of ampicillin. Overnight cultures were diluted in PYE media, grown to 0.1 OD600, and treated with 40 µg/mL ampicillin for 4 h. Cells were collected, washed with minimal media, and incubated with 0.5 mM HADA hydrochloride for 15 min at 30°C. Cells were washed with minimal media again and imaged using a Nikon Fluorescence Microscope CFP filter. A representative cell (yellow arrowheads) was selected from each strain condition shown, and their fluorescence profiles were extracted along the cell length axis. Fluorescence intensity (arbitrary units [a.u.]) is plotted against the position along the entire cell length (scale bar = 3 µm).

    HADA hydrochloride purchased from MedChemExpress. Usage Cited in: mBio. 2024 May 16:e0067924.  [Abstract]

    S. aureus cells expressing Gp11 or its mutants were stained with the fluorescent cell wall marker HADA hydrochloride (250 μM; 30 min) and analyzed by phase-contrast and fluorescence microscopy. The results showed that compared to controls, Gp11 overexpression resulted in a more diffuse HADA signal localized to​ multiple intracellular sites, including the cell periphery and partial septa. Scale bars, 2.5 µm.

    • Biological Activity

    • Purity & Documentation

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    Description

    HADA hydrochloride (HCC-Amino-D-alanine hydrochloride) is a blue (λem~450 nm) fluorescent D-amino acid (FDAA). FDAAs are efficiently incorporated into the peptidoglycans (PGs) of diverse bacterial species at the sites of PG biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation[1].

    In Vitro

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
    1. Solution preparation
    1.1 Preparation of stock solution
    Solvent: DMSO
    Concentration: 250 mM is recommended.
    1.2 Preparation of working solution
    Dilute to 250 μM with cell culture medium (optimized according to the experiment).
    Note: The working solution should be prepared and used immediately. Keep it away from light.

    2. S. aureus strains staining
    2.1 Inoculate the S. aureus strain in a culture dish.
    2.2 Add HADA hydrochloride (250 μM) to the cells.
    2.3 Incubate cells at room temperature or 37 °C for 30 min (optimized according to the experiment).
    2.4 Aspirate the dye working solution, wash 2-3 times with culture medium, 5 minutes each time, and observe using a fluorescence microscope.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    HADA hydrochloride Related Antibodies
    Molecular Weight

    328.71

    Formula

    C13H13ClN2O6
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    N[C@H](CNC(C1=CC2=CC=C(O)C=C2OC1=O)=O)C(O)=O.[H]Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (380.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.0422 mL 15.2110 mL 30.4220 mL
    5 mM 0.6084 mL 3.0422 mL 6.0844 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.0422 mL 15.2110 mL 30.4220 mL 76.0549 mL
    5 mM 0.6084 mL 3.0422 mL 6.0844 mL 15.2110 mL
    10 mM 0.3042 mL 1.5211 mL 3.0422 mL 7.6055 mL
    15 mM 0.2028 mL 1.0141 mL 2.0281 mL 5.0703 mL
    20 mM 0.1521 mL 0.7605 mL 1.5211 mL 3.8027 mL
    25 mM 0.1217 mL 0.6084 mL 1.2169 mL 3.0422 mL
    30 mM 0.1014 mL 0.5070 mL 1.0141 mL 2.5352 mL
    40 mM 0.0761 mL 0.3803 mL 0.7605 mL 1.9014 mL
    50 mM 0.0608 mL 0.3042 mL 0.6084 mL 1.5211 mL
    60 mM 0.0507 mL 0.2535 mL 0.5070 mL 1.2676 mL
    80 mM 0.0380 mL 0.1901 mL 0.3803 mL 0.9507 mL
    100 mM 0.0304 mL 0.1521 mL 0.3042 mL 0.7605 mL
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    HADA hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    HADA2253733-10-5HCC-Amino-D-alanineBacterialFDAAspeptidoglycansprobingbacterialsituInhibitorinhibitorinhibit

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