1. Dye Reagents
  2. WST-1

WST-1 

Cat. No.: HY-136976
Handling Instructions

WST-1 is a Tetrazolium dye, widely used in the cell viability assays. WST-1 produces a water-soluble formazan product (WST-1 formazan) that occurs at the cell surface in NADPH dependent manner in viable cells.

For research use only. We do not sell to patients.

WST-1 Chemical Structure

WST-1 Chemical Structure

CAS No. : 150849-52-8

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
10 mg USD 50 In-stock
Estimated Time of Arrival: December 31
25 mg USD 100 In-stock
Estimated Time of Arrival: December 31
50 mg USD 160 In-stock
Estimated Time of Arrival: December 31
100 mg USD 250 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

WST-1 is a Tetrazolium dye, widely used in the cell viability assays. WST-1 produces a water-soluble formazan product (WST-1 formazan) that occurs at the cell surface in NADPH dependent manner in viable cells[1][2].

In Vitro

WST-1 is used for a wide range of assays that include cell viability, proliferation, plasma membrane electron transport system, superoxide generation, superoxide dismutase, and xanthine oxidase assay[1].
A water-soluble formazan dye is produced from the reduction of WST-1 tetrazolium, of which optical density at 450 nm is measured to evaluate cell viability[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

651.34

Formula

C₁₉H₁₁IN₅NaO₈S₂

CAS No.
SMILES

O=[S]([O-])(C1=C(C=CC([S](=O)(O[Na])=O)=C1)C2=NN(C(C=C3)=CC=C3I)[N+](C(C=C4)=CC=C4[N+]([O-])=O)=N2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (38.38 mM; ultrasonic and warming and heat to 80°C)

H2O : < 0.1 mg/mL (ultrasonic;warming;adjust pH to 9 with NaOH;heat to 80°C) (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5353 mL 7.6765 mL 15.3530 mL
5 mM 0.3071 mL 1.5353 mL 3.0706 mL
10 mM 0.1535 mL 0.7676 mL 1.5353 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (3.84 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.84 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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  • Molarity Calculator

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Keywords:

WST-1WST1WST 1OthersformazancellviabilityNADPHmitochondrialdehydrogenaseInhibitorinhibitorinhibit

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WST-1
Cat. No.:
HY-136976
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