Temocillin 

Temocillin is a derivative of Ticarcillin (HY-100577) and a β-lactamase-resistant Antibiotic. Temocillin blocks water molecules from entering the active sites of Ambler class A and class C serine-dependent β-lactamases, resisting hydrolysis by these enzymes. Temocillin exhibits antibacterial activity against Enterobacteriaceae and Neisseria gonorrhoeae. Temocillin shows antagonistic effects against some isolated strains when used in combination with Ticarcillin (HY-100577) or Cefazolin (HY-B1892). Temocillin can be used in research related to sepsis, urinary tract infections, and lower respiratory tract infections^[1][2][3].

Temocillin exhibits potent activity against wild-type Enterobacteriaceae (MIC₅₀ 2-16 mg/L, MIC₉₀ 4-32 mg/L) and Neisseria gonorrhoeae (MIC₅₀ 0.5 mg/L, MIC₉₀ 1 mg/L), but no activity against Gram-positive cocci, Pseudomonas aeruginosa, or Acinetobacter spp., and acts as a bactericidal agent against Enterobacteriaceae with MBC values equivalent to or twofold higher than MIC values^[1].
Temocillin is highly active against ESBL-, CTX-M-, non-CTX-M ESBL-, dAmpC-, and KPC-producing Enterobacteriaceae (≤99% susceptible at MIC₅₀ ≤32 mg/L), but has minimal activity against OXA-48- and VIM/NDM/IMP-producing Enterobacteriaceae^[1].
Temocillin shows a mild inoculum effect against β-lactamase-negative or ESBL/KPC-producing Enterobacteriaceae, but a marked inoculum effect (8- to 16-fold MBC increase) against chromosomic AmpC β-lactamase-producing Enterobacteriaceae^[1].
Temocillin shows in vitro activity against susceptible and KPC-producing E. coli CFT073 strains with MIC₅₀-like MIC values of 8, 16, and 32 mg/L, respectively, but has minimal activity against OXA-48-producing E. coli CFT073 with an MIC of 256 mg/L^[2].\n\nWait, correction: The original text uses "MICs" and "MIC" which are potency indicators, so they should be bolded with subscript if applicable, but since it's just MIC (minimum inhibitory concentration), which is a potency indicator, we format it as MIC. Also, fix the earlier error where I incorrectly added MIC₅₀ which wasn't in the original. Here's the correct formatted text:\n\nTemocillin shows in vitro activity against susceptible and KPC-producing E. coli CFT073 strains with MICs of 8, 16, and 32 mg/L, respectively, but has minimal activity against OXA-48-producing E. coli CFT073 with an MIC of 256 mg/L^[2].
Temocillin (18-24 h) potently inhibits most β-lactamase-producing Gram-negative Enterobacteriaceae, Haemophilus influenzae, and Neisseria gonorrhoeae with MIC₉₀ values ≤16 μg/mL or ≤1 μg/mL, respectively, but is ineffective against Pseudomonas spp., Acinetobacter spp., and all tested Gram-positive bacteria^[3].
Temocillin (0.1 mM; 10-30 min) potently inhibits Escherichia coli TEM-1, Enterobacter cloacae P99, and Morganella morganii β-lactamases, completely blocking cephaloridine hydrolysis by TEM-1 and Morganella sp. enzymes^[3].
Temocillin (30 μg disk; variable dilution) exhibits antagonistic activity with ticarcillin against some Pseudomonas aeruginosa isolates and with cefazolin against multiple bacterial species, but does not antagonize gentamicin or tobramycin^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

414.45

C₁₆H₁₈N₂O₇S₂

66148-78-5

OC(C(C1=CSC=C1)C(N[C@]2([C@]3([H])N([C@H](C(C)(S3)C)C(O)=O)C2=O)OC)=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Alexandre K, et al. Pharmacokinetics and Pharmacodynamics of Temocillin. Clin Pharmacokinet. 2018;57(3):287-296.  [Content Brief]

  [2]. Alexandre K, et al. Activity of temocillin in a lethal murine model of infection of intra-abdominal origin due to KPC-producing Escherichia coli. J Antimicrob Chemother. 2016;71(7):1899-1904.

  [3]. Jules K, et al. Antibacterial activity and beta-lactamase stability of temocillin. Antimicrob Agents Chemother. 1982;22(3):453-460.  [Content Brief]