2. Metabolic Enzyme/Protease Anti-infection
  3. Endogenous Metabolite Influenza Virus HSV VSV
  4. 3-Indoleacetonitrile

3-Indoleacetonitrile  (Synonyms: 3-インドールアセトニトリル)

製品番号: HY-Y0136 純度: 99.92%
COA 取扱説明書 Technical Support

3-Indoleacetonitrile is an indole derivative with anti-influenza activity. 3-Indoleacetonitrile is a plant hormone produced by cruxiferous vegetables. 3-Indoleacetonitrile exerts profound antiviral activity against a broad spectrum of influenza A viruses, HSV-1 and VSV viruses in vitro. 3-Indoleacetonitrile diminishes lung virus titers and alleviates lung lesions in vivo. 3-Indoleacetonitrile induces an increase in mitochondrial antiviral-signaling (MAVS) protein levels. 3-Indoleacetonitrile can be used in research for combating viral infections including COVID-19, HSV-1, and VSV.

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3-Indoleacetonitrile

3-Indoleacetonitrile 構造式

CAS 番号 : 771-51-7

容量 価格（税別） 在庫状況 数量
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 在庫あり
Solution
10 mM * 1 mL in DMSO USD 33 在庫あり
Solid or liquid
500 mg $30 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 2 publication(s) in Google Scholar

Other Forms of 3-Indoleacetonitrile:

3-Indoleacetonitrile 関連抗体

Top Publications Citing Use of Products

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Human Endogenous Metabolite Microbial Metabolite

HSV アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
HSV-1 HSV-2 HSV

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

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製品説明

3-Indoleacetonitrile is an indole derivative with anti-influenza activity. 3-Indoleacetonitrile is a plant hormone produced by cruxiferous vegetables. 3-Indoleacetonitrile exerts profound antiviral activity against a broad spectrum of influenza A viruses, HSV-1 and VSV viruses in vitro. 3-Indoleacetonitrile diminishes lung virus titers and alleviates lung lesions in vivo. 3-Indoleacetonitrile induces an increase in mitochondrial antiviral-signaling (MAVS) protein levels. 3-Indoleacetonitrile can be used in research for combating viral infections including COVID-19, HSV-1, and VSV[1][2].

IC50 & Target

Human Endogenous Metabolite

 

体外実験

3-Indoleacetonitrile (0-1.5 mM, 24 h) results in significant decrease in GFP fluorescence and inhibited viral proliferation in A549 cells infected with H5N6-GFP virus at an MOI of 0.005[1].
3-Indoleacetonitrile (350 μM, 12-36 h) reduces PR8 PB2 and NP protein levels in A549 cells compared to Arbidol[1].
3-Indoleacetonitrile (350 μM, 24 h) exerts potent anti-influenza activity in MDCK cells[1].
3-Indoleacetonitrile (0-350 μM, 9 h) reduces viral NP and PB2 proteins dose-dependently in A549 infected with H5N6[1].
3-Indoleacetonitrile (0-350 μM, 9 h) impairs PB2 and NP synthesis of PR8, H1N1, H3N2, and California09 H1N1 strains infected A549 cells[1].
3-Indoleacetonitrile (320-640 μM, 24 h) significantly decreases the densities of GFP fluorescence of VSV or HSV-1 and inhibits viral proliferation in Vero E6 and 293T cells[2].
3-Indoleacetonitrile (0-1.28 mM, 24 h) has EC50 of 86.38 μM in Caco-2 and EC50 of 38.79 μM Huh7.0 cells infected with SARS-CoV-2 at MOI of 1[2].
3-Indoleacetonitrile (0-640 μM, 12 h) activates the IFN signaling pathway in 293T cells co-transfected with pGL4-IFN-β and pRL-TK[2].
3-Indoleacetonitrile (680 μM, 0-24 h) results in dramatically higher ISRE- and NF-κB –responsive luciferase activity in 293T cells[2].
3-Indoleacetonitrile (0-50 μM, 12 h) induces a dose-dependent increase in LC3-II and SQSTM1 protein levels in Vero E6 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3-Indoleacetonitrile 関連抗体

Cell Viability Assay[2]

Cell Line: Vero E6 or 293T cells
Concentration: 320 μM (for Vero E6) or 640 μM (for 293T)
Incubation Time: 24 h
Result: Showed unapparent cytotoxicity in both cell lines up to indicated concentrations.

Immunofluorescence[2]

Cell Line: Caco-2, Huh7.0
Concentration: 80, 160, 320, 640, and 1280 μM for Caco-2 cells; 20, 40, 80, 160, and 320 μM for Huh7.0 cells
Incubation Time: 24 h
Result: Showed obvious decrease in densities of FITC fluorescence in both cell lines.
体内実験

3-Indoleacetonitrile (0.2-20 mg/kg, tail i.v., 10 d) does not alter body weights and health conditions of female BALB/c mice[1].
3-Indoleacetonitrile (20 mg/kg, tail i.v., two doses with the second dose administered 1/3/5/7/9 dpi.) results in less weight loss and increased survival rate of H5N6-infected mice to 45.45% and PR8-infected mice to 50%[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Eight-week-old SPF BALB/c female mice inoculated with 50 μL of 2 LD50 H5N6 viruses[1]
Dosage: 20 mg/kg
Administration: Intravenous injection (i.v.) on tail, two doses with the second dose administered 1/3/5/7/9 dpi.
Result: Significantly reduced the viral load in the lungs of mice.
Reduced lung lesions caused by viral infection.
Was able to restrict influenza replication in mice.
分子量

156.19

分子式

C10H8N2
CAS 番号
Appearance

<33°C Solid,>36°C Liquid

Color

Light yellow to brown

SMILES

N#CCC1=CNC2=C1C=CC=C2
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Pure form -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : ≥ 100 mg/mL (640.25 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 1 mg/mL (6.40 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.4025 mL 32.0123 mL 64.0246 mL
5 mM 1.2805 mL 6.4025 mL 12.8049 mL
10 mM 0.6402 mL 3.2012 mL 6.4025 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (16.01 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (16.01 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.92%

COA (251 KB) HNMR (235 KB) LCMS (97 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 6.4025 mL 32.0123 mL 64.0246 mL 160.0615 mL
5 mM 1.2805 mL 6.4025 mL 12.8049 mL 32.0123 mL
DMSO 10 mM 0.6402 mL 3.2012 mL 6.4025 mL 16.0061 mL
15 mM 0.4268 mL 2.1342 mL 4.2683 mL 10.6708 mL
20 mM 0.3201 mL 1.6006 mL 3.2012 mL 8.0031 mL
25 mM 0.2561 mL 1.2805 mL 2.5610 mL 6.4025 mL
30 mM 0.2134 mL 1.0671 mL 2.1342 mL 5.3354 mL
40 mM 0.1601 mL 0.8003 mL 1.6006 mL 4.0015 mL
50 mM 0.1280 mL 0.6402 mL 1.2805 mL 3.2012 mL
60 mM 0.1067 mL 0.5335 mL 1.0671 mL 2.6677 mL
80 mM 0.0800 mL 0.4002 mL 0.8003 mL 2.0008 mL
100 mM 0.0640 mL 0.3201 mL 0.6402 mL 1.6006 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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3-Indoleacetonitrile Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3-Indoleacetonitrile771-51-7Endogenous MetaboliteInfluenza VirusHSVVSVHerpes simplex virusVesicular Stomatitis VirusAnti-influenzaplant hormoneMAVSHSV-1Inhibitorinhibitorinhibit

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3-Indoleacetonitrile
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