5-Methyltetrahydrofolic acid (Synonyms: 5-Methyl THF; 5-MTHF)

Name: 5-Methyltetrahydrofolic acid
Brand: MedChemExpress
SKU: HY-113046
Rating: 5.0 (9 reviews)

Cat. No.: HY-113046 Purity: 99.77%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

5-Methyltetrahydrofolic acid (5-Methyl THF) is the main circulating form of folic acid in the body and is involved in a variety of biochemical reactions. 5-Methyltetrahydrofolic acid regulates cardiovascular function by increasing the production of endothelin-1 (ET-1) in low-density lipoprotein-treated endothelial cells and can be used in the study of cardiovascular diseases.

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CAS No. : 134-35-0

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
500 mg	해외재고보유	Estimated Time of Arrival: December 31
1 g	견적 받기
5 g	견적 받기

or 대량구매 문의

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 9 publication(s) in Google Scholar

Other Forms of 5-Methyltetrahydrofolic acid:

5-Methyltetrahydrofolic acid-¹³C₅ In-stock
5-Methyltetrahydrofolic acid-d₃ In-stock
5-Methyltetrahydrofolic acid disodium In-stock
Calcium N5-methyltetrahydrofolate In-stock
5-Methyltetrahydrofolic acid (Standard) In-stock
5-Methyltetrahydrofolic acid-¹³C₆ 견적 받기
5-Methyltetrahydrofolic acid-d₄ 견적 받기

9 Publications Citing Use of MCE 5-Methyltetrahydrofolic acid

Bio/Physico-chemical Assay

RT-PCR

Flow Cytometry

: 5-Methyltetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2023 Jan 25;51(2):952-965. [Abstract]; Thermogram of a representative ITC experiment of THF-II-loti62 binding to THF (top) and overlay of integrated fitted heat plots of THF-II-loti62 binding to THF, NPR, H4B, 7DG, folinic acid, 5-Methyltetrahydrofolic acid and 6-biopterin (bottom).

: 5-Methyltetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]; Gene expression levels in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated were determined by RT-qPCR.

: 5-Methyltetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]; Lysates from cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated were analyzed by western blotting.

: 5-Methyltetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]; Concentration analysis of H₂O₂ and MDA in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated.

: 5-Methyltetrahydrofolic acid purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]; Flow cytometry analysis of total intracellular ROS (DCF) and mitochondrial ROS (MitoSOX Red) in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated.

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

5-Methyltetrahydrofolic acid (0, 10, 50 and 100 μM; 24 h) increases the production of ET-1 in endothelial cells in a concentration-dependent manner^[2].
5-Methyltetrahydrofolic acid (100 μM; 24 h) inhibits the production of thiobarbituric acid reactive substances (TBARS) by HUVEC in the presence of low-density lipoprotein^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

459.46

화학식

C₂₀H₂₅N₇O₆

CAS No.

134-35-0

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C(O)CC[C@@H](C(O)=O)NC(C1=CC=C(NCC2N(C)C3=C(NC(N)=NC3=O)NC2)C=C1)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 83.33 mg/mL (181.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1765 mL	10.8823 mL	21.7647 mL
5 mM	0.4353 mL	2.1765 mL	4.3529 mL
10 mM	0.2176 mL	1.0882 mL	2.1765 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.77%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (245 KB) HNMR (316 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Wright AJ, et al. Comparison of (6 S)-5-methyltetrahydrofolic acid v. folic acid as the reference folate in longer-term human dietary intervention studies assessing the relative bioavailability of natural food folates: comparative changes in folate status following a 16-week placebo-controlled study in healthy adults. Br J Nutr. 2010 Mar;103(5):724-9. [Content Brief]

[2]. Ronco, et al. "5-methyltetrahydrofolic acid stimulates endothelin-1 production in low density lipoprotein-treated human endothelial cells." Nutrition, Metabolism and Cardiovascular Diseases 17.3 (2007): 188-194. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1765 mL	10.8823 mL	21.7647 mL	54.4117 mL
	5 mM	0.4353 mL	2.1765 mL	4.3529 mL	10.8823 mL
	10 mM	0.2176 mL	1.0882 mL	2.1765 mL	5.4412 mL
	15 mM	0.1451 mL	0.7255 mL	1.4510 mL	3.6274 mL
	20 mM	0.1088 mL	0.5441 mL	1.0882 mL	2.7206 mL
	25 mM	0.0871 mL	0.4353 mL	0.8706 mL	2.1765 mL
	30 mM	0.0725 mL	0.3627 mL	0.7255 mL	1.8137 mL
	40 mM	0.0544 mL	0.2721 mL	0.5441 mL	1.3603 mL
	50 mM	0.0435 mL	0.2176 mL	0.4353 mL	1.0882 mL
	60 mM	0.0363 mL	0.1814 mL	0.3627 mL	0.9069 mL
	80 mM	0.0272 mL	0.1360 mL	0.2721 mL	0.6801 mL
	100 mM	0.0218 mL	0.1088 mL	0.2176 mL	0.5441 mL

5-Methyltetrahydrofolic acid Related Classifications

Metabolic Disease Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Methyltetrahydrofolic acid134-35-05-Methyl THF 5-MTHFEndogenous MetaboliteCirculating formsFolic acidEndothelial cellsCardiovascular diseasesInhibitorinhibitorinhibit

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5-Methyltetrahydrofolic acid (Synonyms: 5-Methyl THF; 5-MTHF)

고객리뷰

Other Forms of 5-Methyltetrahydrofolic acid:

5-Methyltetrahydrofolic acid Related Antibodies

9 Publications Citing Use of MCE 5-Methyltetrahydrofolic acid

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

순도&문서

References

고객리뷰

5-Methyltetrahydrofolic acid Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

5-Methyltetrahydrofolic acid Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report