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Cat. No.: HY-137829

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5-Methyltetrahydrofolic acid disodium

Name: 5-Methyltetrahydrofolic acid disodium
Brand: MedChemExpress
SKU: HY-137829
Price: 110.00 USD
Availability: InStock
Rating: 5 (8 reviews)

8 Cited Publications

Customer Review

Based on 8 publication(s) in Google Scholar

5-Methyltetrahydrofolic acid disodium is the metabolite of Folinic acid (Leucovorin) (HY-17556). 5-Methyltetrahydrofolic acid disodium is involved in one-carbon metabolism and the transfer of methyl groups.

For research use only. We do not sell to patients.

Purity: 88%
CAS No.: 68792-52-9
Formula: C₂₀H₂₃N₇Na₂O₆
Molecular Weight:503.42

Storage:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

5-Methyltetrahydrofolic acid disodium Chemical Structure

CAS No.: 68792-52-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity

Description

All Endogenous Metabolite Isoforms :

Chemical Information

CAS No. 68792-52-9
Appearance Solid
Molecular Weight 503.42
Formula C₂₀H₂₃N₇Na₂O₆
Color Light yellow to yellow
SMILES

O=C(O[Na])CCC(C(O[Na])=O)NC(C1=CC=C(NCC2N(C)C3=C(NC(N)=NC3=O)NC2)C=C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (8)

All Bio/Physico-chemical AssayRT-PCRWBFlow Cytometry

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Journal Impact Factor

J Adv Res

A novel zebrafish model unveils folate deficiency disrupts embryonic development via p53-driven cell cycle arrest. [Abstract]

2026 Mar 5:S2090-1232(26)00188-8. PMID: 41794221
Nucleic Acids Res

Structural insights into translation regulation by the THF-II riboswitch. [Abstract]

2023 Jan 25;51(2):952-965. PMID: 36620887

5-Methyltetrahydrofolic acid disodium purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2023 Jan 25;51(2):952-965. [Abstract]

Thermogram of a representative ITC experiment of THF-II-loti62 binding to THF (top) and overlay of integrated fitted heat plots of THF-II-loti62 binding to THF, NPR, H4B, 7DG, folinic acid, 5-Methyltetrahydrofolic acid and 6-biopterin (bottom).
J Transl Med

Mitochondrial folate pathway regulates myofibroblast differentiation and silica-induced pulmonary fibrosis. [Abstract]

2023 Jun 6;21(1):365. PMID: 37280614

5-Methyltetrahydrofolic acid disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]

Gene expression levels in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated were determined by RT-qPCR.

5-Methyltetrahydrofolic acid disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]

Lysates from cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated were analyzed by western blotting.

5-Methyltetrahydrofolic acid disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]

Concentration analysis of H₂O₂ and MDA in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated.

5-Methyltetrahydrofolic acid disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]

Flow cytometry analysis of total intracellular ROS (DCF) and mitochondrial ROS (MitoSOX Red) in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated.
Appl Microbiol Biotechnol

Role of folic acid in regulating gut microbiota and short-chain fatty acids based on an in vitro fermentation model. [Abstract]

2024 Dec;108(1):1-15. PMID: 38175236
Aquac Rep

2025 Apr 15.
J Pharm Pharmacol

Folic acid and its metabolite codetermination for pharmacokinetics with circadian rhythms and evaluation of oral bioavailability. [Abstract]

2019 Nov;71(11):1645-1654. PMID: 31435940
J Pharm Biomed Anal

A rapid LC-MS/MS method for the simultaneous quantification of 10 key folate cycle intermediates in human plasma. [Abstract]

2026 Jan 15:268:117178. PMID: 41061512
bioRxiv

2025 Nov 20.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (266 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Schleyer E, et al., Highly sensitive coupled-column high-performance liquid chromatographic method for the separation and quantitation of the diastereomers of leucovorin and 5-methyltetrahydrofolate in serum and urine. J Chromatogr B Biomed Appl. 1995 Jul 21;669(2):319-30. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Keywords

5-Methyltetrahydrofolic acid disodium

8 Cited Publications

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Biological Activity
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Chemical Information
Publications (8)
Purity & Documentation
References

1/16
1/16

5-Methyltetrahydrofolic acid disodium purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2023 Jan 25;51(2):952-965. [Abstract]

Customer Validation
Thermogram of a representative ITC experiment of THF-II-loti62 binding to THF (top) and overlay of integrated fitted heat plots of THF-II-loti62 binding to THF, NPR, H4B, 7DG, folinic acid, 5-Methyltetrahydrofolic acid and 6-biopterin (bottom).
1/16

5-Methyltetrahydrofolic acid disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]

Customer Validation
Gene expression levels in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated were determined by RT-qPCR.
1/16

5-Methyltetrahydrofolic acid disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]

Customer Validation
Lysates from cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated were analyzed by western blotting.
1/16

5-Methyltetrahydrofolic acid disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]

Customer Validation
Concentration analysis of H₂O₂ and MDA in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated.
1/16

5-Methyltetrahydrofolic acid disodium purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]

Customer Validation
Flow cytometry analysis of total intracellular ROS (DCF) and mitochondrial ROS (MitoSOX Red) in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated.

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5-Methyltetrahydrofolic acid disodium

Publications Citing Use of MedChemExpress (MCE) 5-Methyltetrahydrofolic acid disodium

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (8)

Purity & Documentation

References

Calculators

Help & FAQs

5-Methyltetrahydrofolic acid disodium Related Classifications

HY-137829 Related Classifications

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