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  4. Calcium N5-methyltetrahydrofolate

Calcium N5-methyltetrahydrofolate  (Synonyms: NSC173328; 5-Methyl THF calcium)

Cat. No.: HY-17557 Purity: 99.85%
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Calcium N5-methyltetrahydrofolate (NSC173328; 5-Methyl THF (calcium)), Levomefolic acid (HY-14781) derivative, is a folic acid supplement. Calcium N5-methyltetrahydrofolate can be used for the research of neural tube defects, infertility, non-hodgkin's lymphoma, endometriosis.

For research use only. We do not sell to patients.

CAS No. : 26560-38-3

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Calcium N5-methyltetrahydrofolate:

Calcium N5-methyltetrahydrofolate Related Antibodies

Top Publications Citing Use of Products

    Calcium N5-methyltetrahydrofolate purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2023 Jan 25;51(2):952-965.  [Abstract]

    Thermogram of a representative ITC experiment of THF-II-loti62 binding to THF (top) and overlay of integrated fitted heat plots of THF-II-loti62 binding to THF, NPR, H4B, 7DG, folinic acid, 5-Methyltetrahydrofolic acid and 6-biopterin (bottom).

    Calcium N5-methyltetrahydrofolate purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365.  [Abstract]

    Gene expression levels in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated were determined by RT-qPCR.

    Calcium N5-methyltetrahydrofolate purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365.  [Abstract]

    Lysates from cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated were analyzed by western blotting.

    Calcium N5-methyltetrahydrofolate purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365.  [Abstract]

    Concentration analysis of H2O2 and MDA in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated.

    Calcium N5-methyltetrahydrofolate purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365.  [Abstract]

    Flow cytometry analysis of total intracellular ROS (DCF) and mitochondrial ROS (MitoSOX Red) in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Calcium N5-methyltetrahydrofolate (NSC173328; 5-Methyl THF (calcium)), Levomefolic acid (HY-14781) derivative, is a folic acid supplement. Calcium N5-methyltetrahydrofolate can be used for the research of neural tube defects, infertility, non-hodgkin's lymphoma, endometriosis[1].

    In Vitro

    Calcium N5-methyltetrahydrofolate (Compound 5-MTHF) effectively bypasses folate insufficiency due to MTHFR deficiency in cells with low MTHFR activity[1].
    Calcium N5-methyltetrahydrofolate uptake by human umbilical vascular endothelial cells is inhibited by Folic acid (HY-16637)[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Calcium N5-methyltetrahydrofolate Related Antibodies
    Clinical Trial
    Molecular Weight

    497.52

    Formula

    C20H23CaN7O6
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    O=C([O-])CC[C@@H](C([O-])=O)NC(C1=CC=C(NCC2N(C)C3=C(N=C(N)NC3=O)NC2)C=C1)=O.[Ca+2]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 18.18 mg/mL (36.54 mM; ultrasonic and warming and adjust pH to 2 with 1 M HCl and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0100 mL 10.0498 mL 20.0997 mL
    5 mM 0.4020 mL 2.0100 mL 4.0199 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 2.0100 mL 10.0498 mL 20.0997 mL 50.2492 mL
    5 mM 0.4020 mL 2.0100 mL 4.0199 mL 10.0498 mL
    10 mM 0.2010 mL 1.0050 mL 2.0100 mL 5.0249 mL
    15 mM 0.1340 mL 0.6700 mL 1.3400 mL 3.3499 mL
    20 mM 0.1005 mL 0.5025 mL 1.0050 mL 2.5125 mL
    25 mM 0.0804 mL 0.4020 mL 0.8040 mL 2.0100 mL
    30 mM 0.0670 mL 0.3350 mL 0.6700 mL 1.6750 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Calcium N5-methyltetrahydrofolate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Calcium N5-methyltetrahydrofolate26560-38-3NSC1733285-Methyl THFNSC 173328NSC-173328Drug DerivativeDrug derivativenon-hodgkin's lymphomahuman umbilical vascular endothelial cellsendometriosisN5-methyltetrahydrofolateone-carbon cyclemethionine synthaseinfertilitymethionineneural tube defectshomocysteineInhibitorinhibitorinhibit

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