Calcium N5-methyltetrahydrofolate
Based on 9 publication(s) in Google Scholar
Calcium N5-methyltetrahydrofolate (NSC173328; 5-Methyl THF (calcium)), Levomefolic acid (HY-14781) derivative, is a folic acid supplement. Calcium N5-methyltetrahydrofolate can be used for the research of neural tube defects, infertility, non-hodgkin's lymphoma, endometriosis.
For research use only. We do not sell to patients.
- Purity: 99.85%
- CAS No.: 26560-38-3
- Formula: C20H23CaN7O6
- Molecular Weight:497.52
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Calcium N5-methyltetrahydrofolate
More- J Adv Res. 2026 Mar 5:S2090-1232(26)00188-8. [Abstract]
- Nucleic Acids Res. 2023 Jan 25;51(2):952-965. [Abstract]
- J Transl Med. 2023 Jun 6;21(1):365. [Abstract]
- Int J Pharm. 2019 Feb 10:556:89-96. [Abstract]
- Appl Microbiol Biotechnol. 2024 Dec;108(1):1-15. [Abstract]
- Aquac Rep. 2025 Apr 15.
- J Pharm Pharmacol. 2019 Nov;71(11):1645-1654. [Abstract]
- J Pharm Biomed Anal. 2026 Jan 15:268:117178. [Abstract]
- bioRxiv. 2025 Nov 20.
Customer Validation & Images
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Bio/Physico-chemical Assay
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RT-PCR
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WB
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Bio/Physico-chemical Assay
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Flow Cytometry
Biological Activity
Calcium N5-methyltetrahydrofolate (Compound 5-MTHF) effectively bypasses folate insufficiency due to MTHFR deficiency in cells with low MTHFR activity[1].
Calcium N5-methyltetrahydrofolate uptake by human umbilical vascular endothelial cells is inhibited by Folic acid (HY-16637)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 26560-38-3
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Appearance Solid
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Molecular Weight 497.52
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Formula C20H23CaN7O6
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Color Off-white to light yellow
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SMILES
O=C([O-])CC[C@@H](C([O-])=O)NC(C1=CC=C(NCC2N(C)C3=C(N=C(N)NC3=O)NC2)C=C1)=O.[Ca+2]
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Synonyms
NSC173328; 5-Methyl THF calcium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (9)
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Journal Impact Factor
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Most Recent
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J Adv Res
A novel zebrafish model unveils folate deficiency disrupts embryonic development via p53-driven cell cycle arrest. [Abstract]2026 Mar 5:S2090-1232(26)00188-8. PMID: 41794221 -
Nucleic Acids Res
2023 Jan 25;51(2):952-965. PMID: 36620887
Calcium N5-methyltetrahydrofolate purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2023 Jan 25;51(2):952-965. [Abstract]
Thermogram of a representative ITC experiment of THF-II-loti62 binding to THF (top) and overlay of integrated fitted heat plots of THF-II-loti62 binding to THF, NPR, H4B, 7DG, folinic acid, 5-Methyltetrahydrofolic acid and 6-biopterin (bottom).
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J Transl Med
Mitochondrial folate pathway regulates myofibroblast differentiation and silica-induced pulmonary fibrosis. [Abstract]2023 Jun 6;21(1):365. PMID: 37280614
Calcium N5-methyltetrahydrofolate purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]
Gene expression levels in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated were determined by RT-qPCR.
Calcium N5-methyltetrahydrofolate purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]
Lysates from cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated were analyzed by western blotting.
Calcium N5-methyltetrahydrofolate purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]
Concentration analysis of H2O2 and MDA in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated.
Calcium N5-methyltetrahydrofolate purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Jun 6;21(1):365. [Abstract]
Flow cytometry analysis of total intracellular ROS (DCF) and mitochondrial ROS (MitoSOX Red) in cells treated with 5-Methyltetrahydrofolic acid (50 μM, 12 h) and TGF-β1 as indicated.
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Int J Pharm
A "Ship-in-a-Bottle" strategy to create folic acid nanoclusters inside the nanocages of γ-cyclodextrin metal-organic frameworks. [Abstract]2019 Feb 10:556:89-96. PMID: 30528633 -
Appl Microbiol Biotechnol
Role of folic acid in regulating gut microbiota and short-chain fatty acids based on an in vitro fermentation model. [Abstract]2024 Dec;108(1):1-15. PMID: 38175236 -
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J Pharm Pharmacol
Folic acid and its metabolite codetermination for pharmacokinetics with circadian rhythms and evaluation of oral bioavailability. [Abstract]2019 Nov;71(11):1645-1654. PMID: 31435940 -
J Pharm Biomed Anal
A rapid LC-MS/MS method for the simultaneous quantification of 10 key folate cycle intermediates in human plasma. [Abstract]2026 Jan 15:268:117178. PMID: 41061512 -
Solvent & Solubility
H2O : 18.18 mg/mL (36.54 mM; ultrasonic and warming and adjust pH to 2 with 1 M HCl and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 2.0100 mL | 10.0498 mL | 20.0997 mL | 50.2492 mL |
| 5 mM | 0.4020 mL | 2.0100 mL | 4.0199 mL | 10.0498 mL | |
| 10 mM | 0.2010 mL | 1.0050 mL | 2.0100 mL | 5.0249 mL | |
| 15 mM | 0.1340 mL | 0.6700 mL | 1.3400 mL | 3.3499 mL | |
| 20 mM | 0.1005 mL | 0.5025 mL | 1.0050 mL | 2.5125 mL | |
| 25 mM | 0.0804 mL | 0.4020 mL | 0.8040 mL | 2.0100 mL | |
| 30 mM | 0.0670 mL | 0.3350 mL | 0.6700 mL | 1.6750 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Calcium N5-methyltetrahydrofolate Related Classifications
HY-17557 Related Classifications
Powered by Bioz
- Calcium N5-methyltetrahydrofolate
- 26560-38-3
- NSC173328
- 5-Methyl THF
- NSC 173328
- NSC-173328
- Drug Derivative
- non-hodgkin's lymphoma
- human umbilical vascular endothelial cells
- endometriosis
- N5-methyltetrahydrofolate
- one-carbon cycle
- methionine synthase
- infertility
- methionine
- neural tube defects
- homocysteine
- Inhibitor
- inhibitor
- inhibit