2. Neuronal Signaling Apoptosis Autophagy
  3. Serotonin Transporter Apoptosis Autophagy
  4. Imipramine hydrochloride

イミプラミン塩酸塩  (Synonyms: Imipramine (hydrochloride))

製品番号: HY-B1490 純度: 99.82%
COA 取扱説明書 Technical Support

Imipramine hydrochloride is an orally active tertiary amine tricyclic antidepressant. Imipramine hydrochloride is a Fascin1 inhibitor with antitumor activities. Imipramine hydrochloride also inhibits serotonin transporter with an IC50 value of 32 nM. Imipramine hydrochloride stimulates U-87MG glioma cells autophagy and induces HL-60 cell apoptosis. Imipramine hydrochloride shows neuroprotective and immunomodulatory effects.

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CAS 番号 : 113-52-0

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 72 在庫あり
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10 mM * 1 mL in DMSO USD 72 在庫あり
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100 mg $66 在庫あり
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カスタマーレビュー

Based on 6 publication(s) in Google Scholar

Other Forms of Imipramine hydrochloride:

イミプラミン塩酸塩 関連抗体

Top Publications Citing Use of Products

    Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969.  [Abstract]

    Cells were exposed to moclobemide, milnacipran, venlafaxine, escitalopram, amitriptyline, trazodone, fluvoxamine, or Imipramine (0–100 μM concentration) for 24 h. Cell viability was assessed with CCK-8 assays.

    Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969.  [Abstract]

    CCK-8 assays for 24 h, 48 h and 72 h in KYSE30 and KYSE150 cells treated with various concentrations of Imipramine.

    Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969.  [Abstract]

    Colony formation assays in KYSE30 and KYSE150 cells treated with Imipramine (0, 50, or 100 μM, 14 days) . Histograms represent the number of colonies per set of replicates, according to colony formation measurements (n = 3).

    Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969.  [Abstract]

    Imipramine (0, 50, or 100 μM, 48 h). KYSE30 and KYSE150 cells showing migration or invasion in Transwell assays were fixed and stained. Cells that crossed the membranes were counted under a light microscope (10 × ) in three random fields of view.

    Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969.  [Abstract]

    Imipramine (0, 50, or 100 μM, 48 h) induces apoptosis in ESCC cells. ESCC or HET-1A cells were treated with various concentrations of Imipramine for 48 h. Cell apoptosis was detected by flow cytometry with annexin V-FITC/Caspase-3 double staining.

    Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969.  [Abstract]

    ESCC cells were treated with various concentrations of Imipramine (50, 100 μM) for 48 h. Western blotting was performed to assess Bax, Bcl-2, and cleaved-caspase-3 expression.

    Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969.  [Abstract]

    Anti-tumor effect of Imipramine (receiving intraperitoneal injections of 20 mg/kg/day, 15 days) in a xenograft model.Representative images depicting the appearance of tumors at the conclusion of treatment. Tumor volumes were measured every 3 days. Final tumor weights.

    Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969.  [Abstract]

    Imipramine (receiving intraperitoneal injections of 20 mg/kg/day, 15 days). Expression of P62, ATG5-ATG12 complex, Bcl-2, Bax, and Ki-67 was detected by IHC staining in tumor tissues.

    Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2023 May 25;21(1):123.  [Abstract]

    LPS-primed BMDMs were stimulated with Imipramine (40 μM, 12 h). Western blot assessed the expression of caspase-1 and IL-1β in SN and GSDMD cleavage in WCL.

    Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2023 May 25;21(1):123.  [Abstract]

    BMDMs were incubated with LPS and MCC950 and then treated with Imipramine (40 μM, 12 h). The expressions of IL-1β and caspase-1 in SN as well as GSDMD cleavage in WCL were evaluated using western blotting.

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    製品説明

    Imipramine hydrochloride is an orally active tertiary amine tricyclic antidepressant. Imipramine hydrochloride is a Fascin1 inhibitor with antitumor activities. Imipramine hydrochloride also inhibits serotonin transporter with an IC50 value of 32 nM. Imipramine hydrochloride stimulates U-87MG glioma cells autophagy and induces HL-60 cell apoptosis. Imipramine hydrochloride shows neuroprotective and immunomodulatory effects[1][2][3][4][5].

    IC50 & Target

    Fascin1, Serotonin Autophagy, Apoptosis[1][2][3][5]
    IC50: 32 nM (human placental serotonin transporter)[5]
    体外実験

    Imipramine (0.5-300 μM, 3 days) inhibits HCT-116 cell viability[1].
    Imipramine (20 μM) inhibits cell migration (7 h) and invasion (48 h)[1].
    Imipramine (50 μM, 0-240 min) inhibites the PI3K/Akt/mTOR signaling pathway in U-87MG glioma cells[2].
    Imipramine (60 μM, 24 h) stimulates U-87MG glioma cells autophagy[2].
    Imipramine (80 μM, 24 h) induces HL-60 cell apoptosis[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    イミプラミン塩酸塩 関連抗体

    Cell Viability Assay[1]

    Cell Line: DLD-1, HCT-116, and SW-480
    Concentration: 0.5-300 μM
    Incubation Time: 3 days
    Result: Inhibited cell viability and HCT-116 was more sensitive than DLD-1 and SW-480.

    Cell Migration Assay [1]

    Cell Line: SW-480, DLD-1, and HCT-116
    Concentration: 20 μM
    Incubation Time: 7 h
    Result: Produced a remarkable inhibition of migration in all assayed cell lines.

    Cell Invasion Assay[1]

    Cell Line: HCT-116
    Concentration: 20 μM
    Incubation Time: 48 h
    Result: Inhibited cell invasion through Matrigel.

    Western Blot Analysis[2]

    Cell Line: U-87MG
    Concentration: 50 μM
    Incubation Time: 0, 15, 30, 60, 120 and 240 min
    Result: Markedly inhibited the phosphorylation of both Akt (Ser473) and mTOR (Ser2481) in a time-dependent manner. Also dephosphorylated p70 S6K, a downstream target of mTOR.

    Cell Autophagy Assay[2]

    Cell Line: U-87MG
    Concentration: 60 μM
    Incubation Time: 24 h
    Result: Stimulated the induction of autophagy through the redistribution of LC3 in U-87MG glioma cells.

    Cell Autophagy Assay[3]

    Cell Line: HL-60
    Concentration: 80 μM
    Incubation Time: 24 h
    Result: Induced cell apoptosis.
    体内実験

    Imipramine (20 mg/kg, i.p. or 15 mg/kg, p.o.; daily for 24 days) attenuates neuroinflammatory signaling and reverses stress-induced social avoidance in mice[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male C57BL/6 mice (6–8 weeks old) subjected to RSD (repeated social defeat) and HCC (home cage control)[4]
    Dosage: 20 mg/kg or 15 mg/kg
    Administration: Intraperitoneal injection or oral administration, daily for 24 days
    Result: Reversed RSD-induced social avoidance behavior, significantly increasing the interaction time, significantly decreased stress-induced mRNA levels for IL-6 in brain microglia.
    分子量

    316.87

    分子式

    C19H25ClN2
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CN(C)CCCN1C2=CC=CC=C2CCC3=CC=CC=C31.[H]Cl
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 100 mg/mL (315.59 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 62.5 mg/mL (197.24 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1559 mL 15.7793 mL 31.5587 mL
    5 mM 0.6312 mL 3.1559 mL 6.3117 mL
    10 mM 0.3156 mL 1.5779 mL 3.1559 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

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    一般には略語で表示されます：C1V1 = C2V2

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    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.89 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (7.89 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 60 mg/mL (189.35 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.92%

    COA (256 KB) HNMR (255 KB) LCMS (103 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.1559 mL 15.7793 mL 31.5587 mL 78.8967 mL
    5 mM 0.6312 mL 3.1559 mL 6.3117 mL 15.7793 mL
    10 mM 0.3156 mL 1.5779 mL 3.1559 mL 7.8897 mL
    15 mM 0.2104 mL 1.0520 mL 2.1039 mL 5.2598 mL
    20 mM 0.1578 mL 0.7890 mL 1.5779 mL 3.9448 mL
    25 mM 0.1262 mL 0.6312 mL 1.2623 mL 3.1559 mL
    30 mM 0.1052 mL 0.5260 mL 1.0520 mL 2.6299 mL
    40 mM 0.0789 mL 0.3945 mL 0.7890 mL 1.9724 mL
    50 mM 0.0631 mL 0.3156 mL 0.6312 mL 1.5779 mL
    60 mM 0.0526 mL 0.2630 mL 0.5260 mL 1.3149 mL
    80 mM 0.0394 mL 0.1972 mL 0.3945 mL 0.9862 mL
    100 mM 0.0316 mL 0.1578 mL 0.3156 mL 0.7890 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Imipramine hydrochloride Related Classifications

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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Imipramine113-52-0Serotonin TransporterApoptosisAutophagy5-HTTSERTSLC6A4colorectal cancer cellsevere chronic depressionimmunomodulatoryneuroprotectiveInhibitorinhibitorinhibit

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