2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Methylmalonic acid

メチルマロン酸  (Synonyms: Methylmalonic acid; Methylpropanedioic acid; Methylmalonate)

製品番号: HY-103395 純度: 98.0%
COA 取扱説明書 Technical Support

Methylmalonic acid (Methylmalonate) is an indicator of Vitamin B-12 deficiency in cancer.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 516-05-2

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 27 在庫あり
Solution
10 mM * 1 mL in DMSO USD 27 在庫あり
Solid
500 mg $25 在庫あり
1 g $40 在庫あり
5 g $65 在庫あり
10 g   お問い合わせ  
50 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 3 publication(s) in Google Scholar

Other Forms of Methylmalonic acid:

メチルマロン酸 関連抗体

Top Publications Citing Use of Products
Bio/Physico-chemical Assay
Cell Imaging/Staining
Cell Proliferation/Viability Assay

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Genom. 2025 Apr 24:100855.  [Abstract]

    Relative abundance of methylmalonate in hepatocytes. CON, control; OE, adenovirus-based Acsf3 overexpression; MMA, methylmalonic acid.

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Genom. 2025 Apr 24:100855.  [Abstract]

    Respiratory rate of methylmalonic acid in hepatocytes. CON, control group; OE, adenovirus-based Acsf3 overexpression group; MMA, methylmalonic acid.

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Genom. 2025 Apr 24:100855.  [Abstract]

    Respiratory rates in hepatocytes. MMA, 5 mM methylmalonate. N = 3. CON, control mice (Acsf3+/−); KO, knockout mice (Acsf3−/−).

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2024 Jan 18;22(1):53.  [Abstract]

    In vitro, AC16 cells were treated with Methylmalonic acid (MMA, 17.5 mM) for 3 h and employed for further analyses. Morphology was evaluated by light microscopy after AC16 cells were treated with MMA.

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2024 Jan 18;22(1):53.  [Abstract]

    In vitro, AC16 cells were treated with Methylmalonic acid (MMA, 17.5 mM) for 3 h and employed for further analyses. AC16 cells were treated with/without MMA in Normoxia or hypoxia-reoxygenation (H/R) condition. Cell viability was detected by 3-(4,5-dimthyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazoliumbromide (MTT) assay.

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2024 Jan 18;22(1):53.  [Abstract]

    In vitro, AC16 cells were treated with Methylmalonic acid (MMA, 17.5 mM) for 3 h and employed for further analyses. Representative TEM images of AC16 cells treated with/without MMA. Yellow arrows indicate mitochondria.

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2024 Jan 18;22(1):53.  [Abstract]

    Methylmalonic acid (MMA)-mediated ROS contributes to cardiomyocytes injury. In vitro, AC16 cells were treated with MMA (17.5 mM) for 3 h or H2O2 (600 μM) for 6 h and then used for further analyses. ROS production was observed by DHE and DCFH-DA staining.

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Methylmalonic acid (Methylmalonate) is an indicator of Vitamin B-12 deficiency in cancer.

    IC50 & Target

    Human Endogenous Metabolite

     

    体外実験

    Vitamin B-12 is required for Methylmalonate (Methylmalonic acid; MMA) to be converted to succinyl-CoA, and in combination with folic acid, for homocysteine (HC) to be converted to methionine. Therefore, Methylmalonate is clearly more specific to vitamin B-12 deficiency compared to HC[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    メチルマロン酸 関連抗体
    臨床実験
    分子量

    118.09

    分子式

    C4H6O4
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(O)C(C)C(O)=O
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (846.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (846.81 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 8.4681 mL 42.3406 mL 84.6812 mL
    5 mM 1.6936 mL 8.4681 mL 16.9362 mL
    10 mM 0.8468 mL 4.2341 mL 8.4681 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (21.17 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (21.17 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (846.81 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 98.0%

    COA (244 KB) HNMR (227 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 8.4681 mL 42.3406 mL 84.6812 mL 211.7029 mL
    5 mM 1.6936 mL 8.4681 mL 16.9362 mL 42.3406 mL
    10 mM 0.8468 mL 4.2341 mL 8.4681 mL 21.1703 mL
    15 mM 0.5645 mL 2.8227 mL 5.6454 mL 14.1135 mL
    20 mM 0.4234 mL 2.1170 mL 4.2341 mL 10.5851 mL
    25 mM 0.3387 mL 1.6936 mL 3.3872 mL 8.4681 mL
    30 mM 0.2823 mL 1.4114 mL 2.8227 mL 7.0568 mL
    40 mM 0.2117 mL 1.0585 mL 2.1170 mL 5.2926 mL
    50 mM 0.1694 mL 0.8468 mL 1.6936 mL 4.2341 mL
    60 mM 0.1411 mL 0.7057 mL 1.4114 mL 3.5284 mL
    80 mM 0.1059 mL 0.5293 mL 1.0585 mL 2.6463 mL
    100 mM 0.0847 mL 0.4234 mL 0.8468 mL 2.1170 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Methylmalonic acid Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Methylmalonic acid516-05-2Methylpropanedioic acid MethylmalonateEndogenous MetaboliteInhibitorinhibitorinhibit

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    製品名:
    Methylmalonic acid
    製品番号:
    HY-103395
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