チミジン (Synonyms: Thymidine; DThyd; NSC 21548)

Name: Thymidine
Brand: MedChemExpress
SKU: HY-N1150
Rating: 5.0 (24 reviews)

製品番号: HY-N1150 純度: 99.94%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication.

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CAS 番号 : 50-89-5

容量	価格（税別）	在庫状況	数量
500 mg	$55	在庫あり	Estimated Time of Arrival: December 31
1 g	$66	在庫あり	Estimated Time of Arrival: December 31
5 g		お問い合わせ
10 g		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 24 publication(s) in Google Scholar

Other Forms of Thymidine:

Thymidine-d₃ 在庫あり
Thymidine-d₄ 在庫あり
Thymidine-¹³C₁₀ 在庫あり
Thymidine-¹³C 在庫あり
Thymidine-¹³C₁₀,¹⁵N₂ 在庫あり
Thymidine-¹⁵N₂ 在庫あり
Thymidine-¹³C₅ 在庫あり
Thymidine (Standard) 在庫あり
Thymidine-d₁₄ お問い合わせ
Thymidine-¹³C-1 お問い合わせ
Thymidine-¹³C-2 お問い合わせ
Thymidine-2′-¹³C お問い合わせ
Thymidine-d₂ お問い合わせ
Thymidine-1¹³C,¹⁵N₂ お問い合わせ

顧客検証

Flow Cytometry

Cell Imaging/Staining

Cell Proliferation/Viability Assay

: Thymidine purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Sep 29;16(1):673. [Abstract]; Thymidine (2.5 mM, 16 h). Immunoblots of TUFT1 in synchronous HeLa cells. Cyclin B1 served as a marker for M phase. GAPDH served as a loading control.

: Thymidine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Dec;11(45):e2405441. [Abstract]; IB analysis of WCL derived from T24 cells synchronized at the late G1/S boundary by double-thymidine block, following by releasing back into the cell cycle for the indicated times.

: Thymidine purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2024 May 20. [Abstract]; Cell viability in day 2 and UMP and dTMP levels were examined in shCtrl and shHES4 A549 cells supplemented with or without 200 μM choline chloride, 50 μM betaine, 2 mM serine, 3 mM sodium pyruvate and 100 μM each of uridine (U) and thymidine (T) for 24 has indicated.

: Thymidine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Aug;9(22):e2104823. [Abstract]; Thymidine (2 mM, 16 h). KG1A cells were synchronized at the G1/S boundary, released into fresh media, and harvested at the indicated times (T/T release).

: Thymidine purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2022 Jan 5:421:126815.; Chromosome segregation image in live HTR-8 cells in control or Thymidine (2 nM, 16 h) treatment and progesterone treatment (after Thymidine treatment).

DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Human Endogenous Metabolite Microbial Metabolite

生物活性
純度とドキュメンテーション
参考文献
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製品説明

IC₅₀ & Target^[2]

Microbial Metabolite

DNA Synthesis

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
143B	CC₅₀	0.6 2 Compound: Thymidine	In vitro cell cytotoxicity was determined against 143B cell line In vitro cell cytotoxicity was determined against 143B cell line	[PMID: 15027876]
143B	CC₅₀	0.0006 41 Compound: Thymidine	In vitro cell cytotoxicity was determined against 143B cell line In vitro cell cytotoxicity was determined against 143B cell line	[PMID: 15027876]
CCRF-CEM	CC₅₀	10.6 3 Compound: 1i	Cytotoxicity in human CEM/0 cells assessed as reduction in cell viability incubated for 4 to 5 days Cytotoxicity in human CEM/0 cells assessed as reduction in cell viability incubated for 4 to 5 days	[PMID: 32515595]
143B	CC₅₀	0.0007 41 Compound: Thymidine	In vitro cell cytotoxicity was determined against 143B-LTK cell line In vitro cell cytotoxicity was determined against 143B-LTK cell line	[PMID: 15027876]
143B	CC₅₀	0.7 2 Compound: Thymidine	In vitro cell cytotoxicity was determined against 143B-LTK cell line In vitro cell cytotoxicity was determined against 143B-LTK cell line	[PMID: 15027876]
HeLa	IC₅₀	4000 3 Compound: 1	Inhibitory concentration against HeLa cell proliferation after 72 hours of incubation Inhibitory concentration against HeLa cell proliferation after 72 hours of incubation	[PMID: 15582414]
Vero	IC₅₀	0.3 1 Compound: Thymidine	Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay	[PMID: 10464017]
HepG2	CC₅₀	2100 3 Compound: beta-L-dT	Cytotoxicity against human HepG2 cells after 3 days by MTT assay Cytotoxicity against human HepG2 cells after 3 days by MTT assay	[PMID: 17404006]
ATH-8 cell line	ED₅₀	>100 3 Compound: 4a	Protection of ATH8 cells against the cytopathic effect of HIV. Protection of ATH8 cells against the cytopathic effect of HIV.	[PMID: 3497272]
HepG2 2.2.15	EC₅₀	0.3 3 Compound: beta-L-dT	Antiviral activity against HBV in HepG2.2.15 cells assessed as decrease in extracellular viral DNA level by RT-PCR Antiviral activity against HBV in HepG2.2.15 cells assessed as decrease in extracellular viral DNA level by RT-PCR	[PMID: 17404006]
Vero	IC₅₀	0.4 1 Compound: thymidine	Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay	[PMID: 10464017]
HL-60	CC₅₀	1760 3 Compound: beta-L-dT	Cytotoxicity against human HL60 cells after 3 days by MTT assay Cytotoxicity against human HL60 cells after 3 days by MTT assay	[PMID: 17404006]
CCRF-CEM	CC₅₀	10.6 3 Compound: 1i	Cytotoxicity in human CEM/0 cells assessed as reduction in cell viability incubated for 4 to 5 days Cytotoxicity in human CEM/0 cells assessed as reduction in cell viability incubated for 4 to 5 days	[PMID: 32515595]
Vero	IC₅₀	0.3 1 Compound: Thymidine	Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay	[PMID: 10464017]
CCRF-CEM	EC₅₀	>40 3 Compound: 3 (d Thd)	Inhibitory effect against HIV-1 replication in CEM cells. Inhibitory effect against HIV-1 replication in CEM cells.	[PMID: 7538589]
Vero	IC₅₀	0.4 1 Compound: thymidine	Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay	[PMID: 10464017]
HeLa	IC₅₀	4 2 Compound: 1	Inhibitory concentration against HeLa cell proliferation after 72 hours of incubation Inhibitory concentration against HeLa cell proliferation after 72 hours of incubation	[PMID: 15582414]
CCRF-CEM	CC₅₀	10.6 3 Compound: 1i	Cytotoxicity in human CEM/0 cells assessed as reduction in cell viability incubated for 4 to 5 days Cytotoxicity in human CEM/0 cells assessed as reduction in cell viability incubated for 4 to 5 days	[PMID: 32515595]
HepG2	CC₅₀	2100 3 Compound: beta-L-dT	Cytotoxicity against human HepG2 cells after 3 days by MTT assay Cytotoxicity against human HepG2 cells after 3 days by MTT assay	[PMID: 17404006]
CCRF-CEM	EC₅₀	> 40 3 Compound: 3 (d Thd)	Inhibitory effect against HIV-1 replication in CEM cells. Inhibitory effect against HIV-1 replication in CEM cells.	[PMID: 7538589]
HepG2 2.2.15	EC₅₀	0.3 3 Compound: beta-L-dT	Antiviral activity against HBV in HepG2.2.15 cells assessed as decrease in extracellular viral DNA level by RT-PCR Antiviral activity against HBV in HepG2.2.15 cells assessed as decrease in extracellular viral DNA level by RT-PCR	[PMID: 17404006]
HL-60	CC₅₀	1760 3 Compound: beta-L-dT	Cytotoxicity against human HL60 cells after 3 days by MTT assay Cytotoxicity against human HL60 cells after 3 days by MTT assay	[PMID: 17404006]
CCRF-CEM	EC₅₀	> 40 3 Compound: 3 (d Thd)	Inhibitory effect against HIV-2 replication in CEM /TK-(thymidine kinase deficient) cells. Inhibitory effect against HIV-2 replication in CEM /TK-(thymidine kinase deficient) cells.	[PMID: 7538589]
Huh-7	CC₅₀	>200 6 Compound: 6	Cytotoxicity against human HuH7 cells Cytotoxicity against human HuH7 cells	[PMID: 20863701]
CCRF-CEM	EC₅₀	> 40 3 Compound: 3 (d Thd)	Inhibitory effect against HIV-2 replication in CEM cells. Inhibitory effect against HIV-2 replication in CEM cells.	[PMID: 7538589]
KBALB cell line	CC₅₀	0.97 2 Compound: Thymidine	In vitro cell cytotoxicity was determined against KBALB cell line In vitro cell cytotoxicity was determined against KBALB cell line	[PMID: 15027876]
HL-60	CC₅₀	1760 3 Compound: beta-L-dT	Cytotoxicity against human HL60 cells after 3 days by MTT assay Cytotoxicity against human HL60 cells after 3 days by MTT assay	[PMID: 17404006]
KBALB-STK	CC₅₀	0.1 2 Compound: Thymidine	In vitro cell cytotoxicity against KBALB-STK cell lines expressed in HSV-1 TK In vitro cell cytotoxicity against KBALB-STK cell lines expressed in HSV-1 TK	[PMID: 12620076]
KBALB-STK	CC₅₀	0.65 2 Compound: Thymidine	In vitro cell cytotoxicity was determined against KBALB-STK cell line In vitro cell cytotoxicity was determined against KBALB-STK cell line	[PMID: 15027876]
HeLa	IC₅₀	4000 3 Compound: 1	Inhibitory concentration against HeLa cell proliferation after 72 hours of incubation Inhibitory concentration against HeLa cell proliferation after 72 hours of incubation	[PMID: 15582414]
HepG2	CC₅₀	2100 3 Compound: beta-L-dT	Cytotoxicity against human HepG2 cells after 3 days by MTT assay Cytotoxicity against human HepG2 cells after 3 days by MTT assay	[PMID: 17404006]
HepG2 2.2.15	EC₅₀	0.3 3 Compound: beta-L-dT	Antiviral activity against HBV in HepG2.2.15 cells assessed as decrease in extracellular viral DNA level by RT-PCR Antiviral activity against HBV in HepG2.2.15 cells assessed as decrease in extracellular viral DNA level by RT-PCR	[PMID: 17404006]
Huh-7	CC₅₀	> 200 6 Compound: 6	Cytotoxicity against human HuH7 cells Cytotoxicity against human HuH7 cells	[PMID: 20863701]
KBALB-STK	CC₅₀	0.0001 41 Compound: Thymidine	In vitro cell cytotoxicity against KBALB-STK cell lines expressed in HSV-1 TK In vitro cell cytotoxicity against KBALB-STK cell lines expressed in HSV-1 TK	[PMID: 12620076]
KBALB-STK	CC₅₀	0.00065 41 Compound: Thymidine	In vitro cell cytotoxicity was determined against KBALB-STK cell line In vitro cell cytotoxicity was determined against KBALB-STK cell line	[PMID: 15027876]
Vero	IC₅₀	0.3 1 Compound: Thymidine	Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay	[PMID: 10464017]
Vero	IC₅₀	0.4 1 Compound: thymidine	Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay	[PMID: 10464017]

体内実験

Thymidine (500 mg/kg; i.p.; twice a day) completely reverses both Methotrexate- and Tomudex-induced deletion of both CD4⁺Vβ8⁺ and CD8⁺Vβ8⁺ T cells ^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-12 weeks BALB/c mice^[3]
Dosage:	500 mg/kg
Administration:	i.p.; twice a day
Result:	Completely abrogates Methotrexate- and Tomudex-induced deletion of Vβ8⁺ T cells after SEB injection.

臨床実験

分子量

242.23

分子式

C₁₀H₁₄N₂O₅

CAS 番号

50-89-5

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1C[C@H](N2C(NC(C(C)=C2)=O)=O)O[C@@H]1CO

Structure Classification

Others

Initial Source

Microorganisms

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 50 mg/mL (206.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 33.33 mg/mL (137.60 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1283 mL	20.6415 mL	41.2831 mL
5 mM	0.8257 mL	4.1283 mL	8.2566 mL
10 mM	0.4128 mL	2.0642 mL	4.1283 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 20 mg/mL (82.57 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.96%

Select Batch:

データシート (278 KB) SDS (396 KB)

COA (252 KB) LCMS (107 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Chen G, et al. Cell Synchronization by Double Thymidine Block. Bio Protoc. 2018 Sep 5;8(17). [Content Brief]

[2]. FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181(4604):274-5.FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181(4604):274-5. [Content Brief]

[3]. Izeradjene K, et al. Inhibition of thymidine synthesis by folate analogues induces a Fas-Fas ligand-independentdeletion of superantigen-reactive peripheral T cells. Int Immunol. 2001 Jan;13(1):85-93. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.1283 mL	20.6415 mL	41.2831 mL	103.2077 mL
	5 mM	0.8257 mL	4.1283 mL	8.2566 mL	20.6415 mL
	10 mM	0.4128 mL	2.0642 mL	4.1283 mL	10.3208 mL
	15 mM	0.2752 mL	1.3761 mL	2.7522 mL	6.8805 mL
	20 mM	0.2064 mL	1.0321 mL	2.0642 mL	5.1604 mL
	25 mM	0.1651 mL	0.8257 mL	1.6513 mL	4.1283 mL
	30 mM	0.1376 mL	0.6881 mL	1.3761 mL	3.4403 mL
	40 mM	0.1032 mL	0.5160 mL	1.0321 mL	2.5802 mL
	50 mM	0.0826 mL	0.4128 mL	0.8257 mL	2.0642 mL
	60 mM	0.0688 mL	0.3440 mL	0.6881 mL	1.7201 mL
	80 mM	0.0516 mL	0.2580 mL	0.5160 mL	1.2901 mL
	100 mM	0.0413 mL	0.2064 mL	0.4128 mL	1.0321 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Thymidine Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Thymidine50-89-5DThyd NSC 21548NSC21548NSC 21548NSC-21548DNA/RNA SynthesisEndogenous MetaboliteOrthopoxvirusprecursorsynchronizingarrestDNAreplicationInhibitorinhibitorinhibit

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チミジン (Synonyms: Thymidine; DThyd; NSC 21548)

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Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Thymidine Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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チミジン (Synonyms: Thymidine; DThyd; NSC 21548)

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Other Forms of Thymidine:

チミジン 関連抗体

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おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Thymidine Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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