1. Epigenetics
  2. Histone Methyltransferase
  3. UNC 0631

UNC 0631 is a potent histone methyltransferase G9a inhibitor with an IC50 of 4 nM. UNC 0631 potently reduces H3K9me2 levels in MDA-MB-231 cells with an IC50 of 25 nM.

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No. CAS : 1320288-19-4

Tamaño Precio Stock Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Revisión del cliente

Based on 5 publication(s) in Google Scholar

Other Forms of UNC 0631:

Top Publications Citing Use of Products

    UNC 0631 purchased from MedChemExpress. Usage Cited in: mBio. 2025 Mar 19:e0013825.  [Abstract]

    CD14+ monocytes were exposed to 22 pM CyaA for 8 days in the presence or absence of the G9a methyltransferase inhibitor UNC 0631 (160 nM). Monocyte differentiation to macrophages was assessed by analyzing the expression of macrophage markers CD36, CD206, and CD204.
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    Descripciòn

    UNC 0631 is a potent histone methyltransferase G9a inhibitor with an IC50 of 4 nM. UNC 0631 potently reduces H3K9me2 levels in MDA-MB-231 cells with an IC50 of 25 nM[1].

    IC50 & Target[1]

    G9a

    4 nM (IC50)

    Cellular Effect
    Cell Line Type Value Description References
    CWR22R EC50
    2.5 3
    Compound: 7, UNC0631
    Cytotoxicity against human 22Rv1 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human 22Rv1 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    CWR22R IC50
    0.024 3
    Compound: 7, UNC0631
    Inhibition of G9a in human 22Rv1 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human 22Rv1 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    CWR22R IC50
    0.024 3
    Compound: 7, UNC0631
    Inhibition of G9a in human 22Rv1 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human 22Rv1 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    CWR22R EC50
    2.5 3
    Compound: 7, UNC0631
    Cytotoxicity against human 22Rv1 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human 22Rv1 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    HCT-116 EC50
    5.8 3
    Compound: 7, UNC0631
    Cytotoxicity against human HCT116 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human HCT116 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    HCT-116 IC50
    0.051 3
    Compound: 7, UNC0631
    Inhibition of G9a in human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    CWR22R EC50
    2.5 3
    Compound: 7, UNC0631
    Cytotoxicity against human 22Rv1 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human 22Rv1 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    HCT-116 EC50
    6.4 3
    Compound: 7, UNC0631
    Cytotoxicity against p53-deficient human HCT116 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against p53-deficient human HCT116 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    HCT-116 IC50
    0.072 3
    Compound: 7, UNC0631
    Inhibition of G9a in p53-deficient human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in p53-deficient human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    CWR22R IC50
    0.024 3
    Compound: 7, UNC0631
    Inhibition of G9a in human 22Rv1 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human 22Rv1 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    HCT-116 IC50
    0.051 3
    Compound: 7, UNC0631
    Inhibition of G9a in human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    HCT-116 EC50
    5.8 3
    Compound: 7, UNC0631
    Cytotoxicity against human HCT116 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human HCT116 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    HCT-116 IC50
    0.072 3
    Compound: 7, UNC0631
    Inhibition of G9a in p53-deficient human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in p53-deficient human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    HCT-116 EC50
    6.4 3
    Compound: 7, UNC0631
    Cytotoxicity against p53-deficient human HCT116 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against p53-deficient human HCT116 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    HCT-116 EC50
    5.8 3
    Compound: 7, UNC0631
    Cytotoxicity against human HCT116 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human HCT116 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    IMR-90 EC50
    1.8 3
    Compound: 7, UNC0631
    Cytotoxicity against human IMR90 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human IMR90 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    IMR-90 IC50
    0.046 3
    Compound: 7, UNC0631
    Inhibition of G9a in human IMR90 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human IMR90 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    HCT-116 EC50
    6.4 3
    Compound: 7, UNC0631
    Cytotoxicity against p53-deficient human HCT116 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against p53-deficient human HCT116 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    IMR-90 IC50
    0.046 3
    Compound: 7, UNC0631
    Inhibition of G9a in human IMR90 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human IMR90 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    IMR-90 EC50
    1.8 3
    Compound: 7, UNC0631
    Cytotoxicity against human IMR90 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human IMR90 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    HCT-116 IC50
    0.051 3
    Compound: 7, UNC0631
    Inhibition of G9a in human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    MCF7 IC50
    0.018 3
    Compound: 7, UNC0631
    Inhibition of G9a in human MCF7 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human MCF7 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    MCF7 IC50
    0.018 3
    Compound: 7, UNC0631
    Inhibition of G9a in human MCF7 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human MCF7 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    HCT-116 IC50
    0.072 3
    Compound: 7, UNC0631
    Inhibition of G9a in p53-deficient human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in p53-deficient human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    MDA-MB-231 EC50
    2.8 3
    Compound: 7, UNC0631
    Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    MDA-MB-231 IC50
    0.025 3
    Compound: 7, UNC0631
    Inhibition of G9a in human MDA-MB-231 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human MDA-MB-231 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    MDA-MB-231 IC50
    0.025 3
    Compound: 7, UNC0631
    Inhibition of G9a in human MDA-MB-231 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human MDA-MB-231 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    MDA-MB-231 EC50
    2.8 3
    Compound: 7, UNC0631
    Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    IMR-90 EC50
    1.8 3
    Compound: 7, UNC0631
    Cytotoxicity against human IMR90 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human IMR90 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    PC-3 EC50
    3.8 3
    Compound: 7, UNC0631
    Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    PC-3 IC50
    0.026 3
    Compound: 7, UNC0631
    Inhibition of G9a in human PC3 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human PC3 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    PC-3 IC50
    0.026 3
    Compound: 7, UNC0631
    Inhibition of G9a in human PC3 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human PC3 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    IMR-90 IC50
    0.046 3
    Compound: 7, UNC0631
    Inhibition of G9a in human IMR90 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human IMR90 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    PC-3 EC50
    3.8 3
    Compound: 7, UNC0631
    Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    MCF7 IC50
    0.018 3
    Compound: 7, UNC0631
    Inhibition of G9a in human MCF7 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human MCF7 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    MDA-MB-231 EC50
    2.8 3
    Compound: 7, UNC0631
    Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    MDA-MB-231 IC50
    0.025 3
    Compound: 7, UNC0631
    Inhibition of G9a in human MDA-MB-231 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human MDA-MB-231 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    PC-3 EC50
    3.8 3
    Compound: 7, UNC0631
    Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by MTT assay
    Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by MTT assay
    [PMID: 21780790]
    PC-3 IC50
    0.026 3
    Compound: 7, UNC0631
    Inhibition of G9a in human PC3 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    Inhibition of G9a in human PC3 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay
    [PMID: 21780790]
    In Vitro

    UNC 0631 (Compound 7) has high cellular potency and excellent separation of functional potency versus cell toxicity in a variety of cell lines. UNC 0631 is highly potent in reducing H3K9me2 levels and has low cell toxicity. UNC 0631 reduces H3K9me2 levels with ICW IC50 values of 25 nM, 18 nM, 26 nM, 24 nM, 51 nM, 72 nM and 46 nM in MDA-MB-231, MCF7, PC3, 22RV1, HCT116 wt, HCT 116 p53-/- and IMR90 cell lines, respectively[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Peso molecular

    635.93

    Fòrmula

    C37H61N7O2

    No. CAS
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    COC1=CC2=C(NC3CCN(CC4CCCCC4)CC3)N=C(N5CCN(C(C)C)CCC5)N=C2C=C1OCCCN6CCCCC6

    Envío

    Room temperature in continental US; may vary elsewhere.

    Almacenamiento
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvente y solubilidad
    In Vitro: 

    DMF : 150 mg/mL (235.88 mM; Need ultrasonic)

    Ethanol : 100 mg/mL (157.25 mM; Need ultrasonic)

    DMSO : 16.67 mg/mL (26.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5725 mL 7.8625 mL 15.7250 mL
    5 mM 0.3145 mL 1.5725 mL 3.1450 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (3.93 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (3.93 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Pureza y Documentación
    Referencias

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / Ethanol / DMF 1 mM 1.5725 mL 7.8625 mL 15.7250 mL 39.3125 mL
    5 mM 0.3145 mL 1.5725 mL 3.1450 mL 7.8625 mL
    10 mM 0.1573 mL 0.7863 mL 1.5725 mL 3.9313 mL
    15 mM 0.1048 mL 0.5242 mL 1.0483 mL 2.6208 mL
    20 mM 0.0786 mL 0.3931 mL 0.7863 mL 1.9656 mL
    25 mM 0.0629 mL 0.3145 mL 0.6290 mL 1.5725 mL
    Ethanol / DMF 30 mM 0.0524 mL 0.2621 mL 0.5242 mL 1.3104 mL
    40 mM 0.0393 mL 0.1966 mL 0.3931 mL 0.9828 mL
    50 mM 0.0315 mL 0.1573 mL 0.3145 mL 0.7863 mL
    60 mM 0.0262 mL 0.1310 mL 0.2621 mL 0.6552 mL
    80 mM 0.0197 mL 0.0983 mL 0.1966 mL 0.4914 mL
    100 mM 0.0157 mL 0.0786 mL 0.1573 mL 0.3931 mL
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    Nombre del producto:
    UNC 0631
    Cat. No.:
    HY-13808
    Cantidad:
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