2. Epigenetics TGF-beta/Smad
  3. PKC
  4. (-)-Indolactam V

(-)-Indolactam V  (Synonyms: Indolactam V; (-)-インドラクタムV)

製品番号: HY-12307 純度: 98.42%
COA 取扱説明書 Technical Support

(-)-Indolactam V is a PKC activator, with Kis of 3.36 nM, 1.03 μM for η-CRD2 (PKCη surrogate peptide), γ-CRD2 (PKCγ surrogate peptide), and Kds of 5.5 nM (η-C1B), 7.7 nM (ε-C1B), 8.3 nM (δ-C1B), 18.9 nM (β-C1A-long), 20.8 nM (α-C1A-long), 137 nM (β-C1B), 138 nM (γ-C1A), 213 nM (γ-C1B), and has antitumor activity.

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(-)-Indolactam V

(-)-Indolactam V 構造式

CAS 番号 : 90365-57-4

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 798 在庫あり
Solution
10 mM * 1 mL in DMSO USD 798 在庫あり
Solid
1 mg $198 在庫あり
5 mg $726 在庫あり
10 mg $1150 在庫あり
25 mg $2300 在庫あり
50 mg $3680 在庫あり
100 mg $5800 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 2 publication(s) in Google Scholar

(-)-Indolactam V 関連抗体

Top Publications Citing Use of Products

PKC アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

  • 生物活性

  • プロトコル

  • 純度とドキュメンテーション

  • 参考文献

  • カスタマーレビュー

製品説明

(-)-Indolactam V is a PKC activator, with Kis of 3.36 nM, 1.03 μM for η-CRD2 (PKCη surrogate peptide), γ-CRD2 (PKCγ surrogate peptide), and Kds of 5.5 nM (η-C1B), 7.7 nM (ε-C1B), 8.3 nM (δ-C1B), 18.9 nM (β-C1A-long), 20.8 nM (α-C1A-long), 137 nM (β-C1B), 138 nM (γ-C1A), 213 nM (γ-C1B), and has antitumor activity.

IC50 & Target[1][2]

PKCη-CRD2

3.36 nM (Ki)

PKCη-C1B

5.5 nM (Kd)

PKCε-C1B

7.7 nM (Kd)

PKCδ-C1B

8.3 nM (Kd)

PKCθ-C1B

8.7 nM (Kd)

PKCβ-C1A-long

18.9 nM (Kd)

PKCα-C1A-long

20.8 nM (Kd)

PKCβ-C1B

137 nM (Kd)

PKCγ-C1A

138 nM (Kd)

PKCγ-C1B

213 nM (Kd)

PKCγ-CRD2

1030 nM (Ki)

PKCδ-C1A

1900 nM (Kd)

PKCη-C1A

3770 nM (Kd)

PKCα-C1B-long

4000 nM (Kd)

PKCε-C1A

4110 nM (Kd)

Cellular Effect
Cell Line Type Value Description References
HL-60 EC50
5 1
Compound: 3 (Indolactam V)
Effective concentration against monocytes of HL-60 cells growth inhibition
Effective concentration against monocytes of HL-60 cells growth inhibition
[PMID: 9554881]
HL-60 ED50
5.3 × 10-7 48
Compound: 2
Dose inducing HL-60 cell adhesion related to skin tumor promotion.
Dose inducing HL-60 cell adhesion related to skin tumor promotion.
[PMID: 1613749]
HL-60 EC50
5 1
Compound: 3 (Indolactam V)
Effective concentration against monocytes of HL-60 cells growth inhibition
Effective concentration against monocytes of HL-60 cells growth inhibition
[PMID: 9554881]
HL-60 ED50
5.3 48
Compound: 2
Dose inducing HL-60 cell adhesion related to skin tumor promotion.
Dose inducing HL-60 cell adhesion related to skin tumor promotion.
[PMID: 1613749]
PBMC CC50
> 100 3
Compound: Indolactam-V
Toxicity in human PBMC assessed as reduction in cell viability after 5 days by WST8 assay
Toxicity in human PBMC assessed as reduction in cell viability after 5 days by WST8 assay
[PMID: 30413535]
U-937 CC50
> 100 3
Compound: Indolactam-V
Toxicity in human U-937 cells assessed as reduction in cell viability after 48 hrs by WST8 assay
Toxicity in human U-937 cells assessed as reduction in cell viability after 48 hrs by WST8 assay
[PMID: 30413535]
体外実験

(-)-Indolactam V is a PKC activator, with Kis of 3.36 nM, 1.03 μM for η-CRD2 (PKCη surrogate peptide), γ-CRD2 (PKCγ surrogate peptide), and has antitumor activity[1]. (-)-Indolactam V shows Kds of 5.5 nM (η-C1B), 7.7 nM (ε-C1B), 8.3 nM (δ-C1B), 18.9 nM (β-C1A-long), 20.8 nM (α-C1A-long), 137 nM (β-C1B), 138 nM (γ-C1A), 213 nM (γ-C1B), respectively[2]. (-)-Indolactam V (20 nM-5 μM) dose-dependently affects multiple hESC lines, such as HUES 2, 4 and 8. (-)-Indolactam V also increases the mRNA levels of Pdx1, HNF6, PTF1A, SOX9, HB9 and PROX1. In addition, (-)-Indolactam V (300 nM) functions in both mouse and human cells and confirms that some signals for pancreatic development[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(-)-Indolactam V 関連抗体
分子量

301.38

分子式

C17H23N3O2
CAS 番号
Appearance

Solid

Color

White to light yellow

SMILES

CC(C)[C@@H](C1=O)N(C)C2=C3C(NC=C3C[C@H](N1)CO)=CC=C2
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 50 mg/mL (165.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3181 mL 16.5904 mL 33.1807 mL
5 mM 0.6636 mL 3.3181 mL 6.6361 mL
10 mM 0.3318 mL 1.6590 mL 3.3181 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.30 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.30 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 98.75%

COA (253 KB) LCMS (93 KB)

取扱説明書 (2659 KB)
参考文献
細胞実験
[3]

For induced differentiation to endocrine or exocrine cells, the (-)-Indolactam V (300 nM)-treated populations are cultured in DMEM/F12 supplemented with 1 N2, 2 mg/mL albumin fraction V and 10 ng/mL bovine FGF for the first 4 d. 10 mM nicotinamide is then added and maintained for an additional 8 d, changing the medium every 3 d[3].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3181 mL 16.5904 mL 33.1807 mL 82.9518 mL
5 mM 0.6636 mL 3.3181 mL 6.6361 mL 16.5904 mL
10 mM 0.3318 mL 1.6590 mL 3.3181 mL 8.2952 mL
15 mM 0.2212 mL 1.1060 mL 2.2120 mL 5.5301 mL
20 mM 0.1659 mL 0.8295 mL 1.6590 mL 4.1476 mL
25 mM 0.1327 mL 0.6636 mL 1.3272 mL 3.3181 mL
30 mM 0.1106 mL 0.5530 mL 1.1060 mL 2.7651 mL
40 mM 0.0830 mL 0.4148 mL 0.8295 mL 2.0738 mL
50 mM 0.0664 mL 0.3318 mL 0.6636 mL 1.6590 mL
60 mM 0.0553 mL 0.2765 mL 0.5530 mL 1.3825 mL
80 mM 0.0415 mL 0.2074 mL 0.4148 mL 1.0369 mL
100 mM 0.0332 mL 0.1659 mL 0.3318 mL 0.8295 mL
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(-)-Indolactam V Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(-)-Indolactam V90365-57-4Indolactam VPKCProtein kinase CInhibitorinhibitorinhibit

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