2. Metabolic Enzyme/Protease
  3. Phospholipase
  4. BAPTA

BAPTA is a selective and cell-impermeant chelator for calcium. BAPTA has high selectivity against magnesium and calcium. BAPTA can also inhibit phospholipase C activity independently of their role as Ca2+ chelators.

For research use only. We do not sell to patients.

CAS No. : 85233-19-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
Solid
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200 mg In-stock
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Customer Review

Based on 36 publication(s) in Google Scholar

Other Forms of BAPTA:

BAPTA Related Antibodies

Top Publications Citing Use of Products

36 Publications Citing Use of MCE BAPTA

WB
RT-PCR
ELISA
IF

    BAPTA purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2025 Aug 28;22(1):208.  [Abstract]

    BAPTA (10 μM). WB results showed that BAPTA could attenuate the Hb-induced decrease in LAMP1 and m-CTSD/pro-CTSD.

    BAPTA purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2025 Aug 28;22(1):208.  [Abstract]

    IF results showed that BAPTA (10 μM) can partially increase the co-localization coefficients of LAMP1 and CTSD.

    BAPTA purchased from MedChemExpress. Usage Cited in: Mil Med Res. 2023 Nov 25;10(1):56.  [Abstract]

    BAPTA (10 μmol/L, 24 h). qRT-PCR was used to assess the mRNA level of Il6, Tnfα and Tgfβ3 in HMs.

    BAPTA purchased from MedChemExpress. Usage Cited in: Mil Med Res. 2023 Nov 25;10(1):56.  [Abstract]

    BAPTA (10 μmol/L, 24 h). TGF-β1 and IL-6 level in the supernatant of HMs were detected by ELISA.

    BAPTA purchased from MedChemExpress. Usage Cited in: Mil Med Res. 2023 Nov 25;10(1):56.  [Abstract]

    BAPTA (10 μmol/L, 24 h). The expression and location of TNF-α in HMs was assessed by confocal microscopy (c). Scale bar = 20 μm.

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    Phospholipase A PLD Phospholipase C

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    BAPTA is a selective and cell-impermeant chelator for calcium. BAPTA has high selectivity against magnesium and calcium. BAPTA can also inhibit phospholipase C activity independently of their role as Ca2+ chelators[1][2][3].

    IC50 & Target

    Calcium[1]
    In Vitro

    BAPTA is widely used as an intracellular buffer to investigate the effects caused by Ca2+ release from intracellular calcium stores or Ca2+ influx via Ca2+-permeable channels in the plasma membrane[1].
    BAPTA can be introduced into cells via methods such as patch-clamp and microinjection[1].
    BAPTA inhibits PLC activity in isolated Drosophila melanogaster photoreceptor cells with an IC50 of approximately 8 mM[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    BAPTA Related Antibodies
    Molecular Weight

    476.43

    Formula

    C22H24N2O10
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light brown

    SMILES

    O=C(O)CN(C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(O)=O)CC(O)=O)CC(O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    1M NaOH : 33.33 mg/mL (69.96 mM; ultrasonic and adjust pH to 11 with NaOH)

    DMSO : 15.62 mg/mL (32.79 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0989 mL 10.4947 mL 20.9894 mL
    5 mM 0.4198 mL 2.0989 mL 4.1979 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.56 mg/mL (3.27 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.56 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.6 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1.56 mg/mL (3.27 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.56 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.6 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.10%

    SDS (396 KB)

    COA (249 KB) HNMR (267 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / 1M NaOH 1 mM 2.0989 mL 10.4947 mL 20.9894 mL 52.4736 mL
    5 mM 0.4198 mL 2.0989 mL 4.1979 mL 10.4947 mL
    10 mM 0.2099 mL 1.0495 mL 2.0989 mL 5.2474 mL
    15 mM 0.1399 mL 0.6996 mL 1.3993 mL 3.4982 mL
    20 mM 0.1049 mL 0.5247 mL 1.0495 mL 2.6237 mL
    25 mM 0.0840 mL 0.4198 mL 0.8396 mL 2.0989 mL
    30 mM 0.0700 mL 0.3498 mL 0.6996 mL 1.7491 mL
    1M NaOH 40 mM 0.0525 mL 0.2624 mL 0.5247 mL 1.3118 mL
    50 mM 0.0420 mL 0.2099 mL 0.4198 mL 1.0495 mL
    60 mM 0.0350 mL 0.1749 mL 0.3498 mL 0.8746 mL
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    BAPTA Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BAPTA85233-19-8PhospholipaseCa2+Mg2+calcium chelatorDrosophila melanogaster photoreceptorsPLCInhibitorinhibitorinhibit

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