2. Cytoskeleton
  3. Integrin
  4. Cyclo(Arg-Gly-Asp-D-Phe-Cys)

Cyclo(Arg-Gly-Asp-D-Phe-Cys)  (Synonyms: Cyclo(RGDfC))

製品番号: HY-P2300 純度: 98.08%
COA 取扱説明書 Technical Support

Cyclo(Arg-Gly-Asp-D-Phe-Cys) (Cyclo RGDfC), a cyclic RGD peptide which has high affinity to αvβ3, can disrupt cell integrin interactions. Cyclo(Arg-Gly-Asp-D-Phe-Cys) inhibits pluripotent marker expression in embryonic stem cells (ESCs) and the tumorigenic potential of mESCs in vivo. Cyclo(Arg-Gly-Asp-D-Phe-Cys) can be used in the research of tumors.

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Cyclo(Arg-Gly-Asp-D-Phe-Cys)

Cyclo(Arg-Gly-Asp-D-Phe-Cys) 構造式

CAS 番号 : 862772-11-0

容量 価格（税別） 在庫状況 数量
1 mg $70 在庫あり
5 mg $180 在庫あり
10 mg $280 在庫あり
25 mg $480 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 2 publication(s) in Google Scholar

Other Forms of Cyclo(Arg-Gly-Asp-D-Phe-Cys):

Cyclo(Arg-Gly-Asp-D-Phe-Cys) 関連抗体

Top Publications Citing Use of Products

Integrin アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
αvβ1 αvβ3 αvβ5 αvβ6 αvβ8 α5β1 α1β1 α2β1 α4β1 α9β1 αIIbβ3 α4β7 αLβ2

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製品説明

Cyclo(Arg-Gly-Asp-D-Phe-Cys) (Cyclo RGDfC), a cyclic RGD peptide which has high affinity to αvβ3, can disrupt cell integrin interactions. Cyclo(Arg-Gly-Asp-D-Phe-Cys) inhibits pluripotent marker expression in embryonic stem cells (ESCs) and the tumorigenic potential of mESCs in vivo. Cyclo(Arg-Gly-Asp-D-Phe-Cys) can be used in the research of tumors[1].

IC50 & Target[1]

αvβ3

 

体外実験

Cyclo(Arg-Gly-Asp-D-Phe-Cys) (0.5 mM; 24 h) down-regulates the transcription factors Oct 4, Sox 2 and Nanog of mESCs[1].
Cyclo(Arg-Gly-Asp-D-Phe-Cys) (0.5 mM) inhibits integrin gene expression mESC-col I (type I collagen) constructs[1].
Cyclo(Arg-Gly-Asp-D-Phe-Cys) (0.5 mM)-treated mESC leads to the formation of aggregates and detachment from the surface[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cyclo(Arg-Gly-Asp-D-Phe-Cys) 関連抗体

RT-PCR[1]

Cell Line: MESCs
Concentration: 0.5 mM
Incubation Time: 24 h
Result: Down-regulated Oct 4, Nanog, Sox 2 by 99%, 97.5%, and 90% respectively.
Up-regulated Rex1 gene expression.
体内実験

Cyclo(Arg-Gly-Asp-D-Phe-Cys) (0.5 mM, 24 h)-treated mESCs in the presence/absence of Leukemia inhibitory factor (LIF) (injected in both thighs) generated teratomas in severe combined immunodeficiency (SCID) mice, which indicates that the process of mESC tumor formation in vivo is dependent on integrin interaction[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

578.64

分子式

C24H34N8O7S
CAS 番号
Appearance

Solid

Color

White to off-white

Sequence

Cyclo(Arg-Gly-Asp-D-Phe-Cys)
Sequence Shortening

cyclo(RGDfC)
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

polypeptide, sealed storage, away from moisture and light, under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (polypeptide, sealed storage, away from moisture and light, under nitrogen)
溶剤 & 溶解度
体外: 

DMSO : 50 mg/mL (86.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7282 mL 8.6410 mL 17.2819 mL
5 mM 0.3456 mL 1.7282 mL 3.4564 mL
10 mM 0.1728 mL 0.8641 mL 1.7282 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

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体積 （終了）

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体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 98.08%

COA (254 KB) RP-HPLC (255 KB) MS (224 KB) Elemental Analysis Report (114 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7282 mL 8.6410 mL 17.2819 mL 43.2048 mL
5 mM 0.3456 mL 1.7282 mL 3.4564 mL 8.6410 mL
10 mM 0.1728 mL 0.8641 mL 1.7282 mL 4.3205 mL
15 mM 0.1152 mL 0.5761 mL 1.1521 mL 2.8803 mL
20 mM 0.0864 mL 0.4320 mL 0.8641 mL 2.1602 mL
25 mM 0.0691 mL 0.3456 mL 0.6913 mL 1.7282 mL
30 mM 0.0576 mL 0.2880 mL 0.5761 mL 1.4402 mL
40 mM 0.0432 mL 0.2160 mL 0.4320 mL 1.0801 mL
50 mM 0.0346 mL 0.1728 mL 0.3456 mL 0.8641 mL
60 mM 0.0288 mL 0.1440 mL 0.2880 mL 0.7201 mL
80 mM 0.0216 mL 0.1080 mL 0.2160 mL 0.5401 mL
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Cyclo(Arg-Gly-Asp-D-Phe-Cys) Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cyclo(Arg-Gly-Asp-D-Phe-Cys)862772-11-0Cyclo(RGDfC)Integrinαvβ3tumormESCsSCID miceInhibitorinhibitorinhibit

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製品名:
Cyclo(Arg-Gly-Asp-D-Phe-Cys)
製品番号:
HY-P2300
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