1. PI3K/Akt/mTOR
    Stem Cell/Wnt
    MAPK/ERK Pathway
    Autophagy
    Anti-infection
  2. Akt
    ERK
    Autophagy
    HCV
  3. Honokiol

Honokiol (Synonyms: NSC 293100)

Cat. No.: HY-N0003 Purity: 99.90%
Handling Instructions

Honokiol is a bioactive, biphenolic phytochemical that possesses potent antioxidative, anti-inflammatory, antiangiogenic, and anticancer activities by targeting a variety of signaling molecules. It inhibits the activation of Akt and enhances the phosphorylation of ERK1/ERK2. Honokiol can readily cross the blood brain barrier.

For research use only. We do not sell to patients.

Honokiol Chemical Structure

Honokiol Chemical Structure

CAS No. : 35354-74-6

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
50 mg USD 60 In-stock
Estimated Time of Arrival: December 31
100 mg USD 72 In-stock
Estimated Time of Arrival: December 31
200 mg USD 90 In-stock
Estimated Time of Arrival: December 31
500 mg   Get quote  
1 g   Get quote  

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Customer Review

Based on 8 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Honokiol purchased from MCE. Usage Cited in: Int J Biol Macromol. 2020 Jan 7. pii: S0141-8130(19)39993-3.

    Effect of GFP-A and Honokiol (activator of ERK1/2) (10 μmol/L) on the protein expression of NF-κB p65 in HT-29 cells. Cells were treated with 180 μg/mL GFP-A in the presence or absence of Honokiol for different durations of time. The expression of protein was analyzed by western blot. β-Actin was used as an equal loading control.

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    • References

    • Customer Review

    Description

    Honokiol is a bioactive, biphenolic phytochemical that possesses potent antioxidative, anti-inflammatory, antiangiogenic, and anticancer activities by targeting a variety of signaling molecules. It inhibits the activation of Akt and enhances the phosphorylation of ERK1/ERK2. Honokiol can readily cross the blood brain barrier.

    IC50 & Target[4]

    Akt

     

    ERK1

     

    ERK2

     

    Autophagy

     

    In Vitro

    Honokiol (0, 12.5, 25 and 50 μM) inhibits the growth of GBM cells and induces apoptosis, with IC50 of appr against 30 μM DBTRG-05MG cell. Honokiol-induced apoptosis of GBM cells is associated with the downregulation of the Rb protein and cleavage of PARP and Bcl-x (S/L). Honokiol (50 μM) increases the level of autophagy markers in GBM cells[1]. Honokiol has anticancer effect, and the IC50 values with MDA-MB-231, MDA-MB-468, and MDA-MB-453 cell lines is 16.99 ± 1.28 μM, 15.94 ± 2.35 μM and 20.11 ±3.13 μM respectively. Honokiol (3, 10 μM) produces significant inhibition on the spheroid number and spheroid sizes in the clonogenic assay[2]. Honokiol (0.1-1.0 μM) specifically inhibits washed human platelet aggregation stimulated by collagen, but not by other agonists. honokiol (0.6 and 1.0 μM) can concentration-dependently inhibit the collagen-induced ATP-release reaction in washed human platelets. Honokiol specifically inhibits platelet aggregation and the phosphorylation of Lyn, PLCγ2, and PKC stimulated with convulxin. Honokiol (5, 10 μM) significantly inhibits convulxin-stimulated MAPKs and Akt activation[3]. Honokiol (10, 20 μM) increases ERK1/2 phosphorylation in a dose-dependent manner depending on CaMK II activation[4].

    In Vivo

    Honokiol-NM (40 mg/kg, p.o.) produces superior anticancer effects, and the PCNA, Cyclin D1 and cleaved caspase 3 expressions are 2.12, 1.92 and 1.68-fold significantly altered in this treated group[2].

    Molecular Weight

    266.33

    Formula

    C₁₈H₁₈O₂

    CAS No.

    35354-74-6

    SMILES

    OC1=C(CC=C)C=C(C2=CC(CC=C)=CC=C2O)C=C1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 50 mg/mL (187.74 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7547 mL 18.7737 mL 37.5474 mL
    5 mM 0.7509 mL 3.7547 mL 7.5095 mL
    10 mM 0.3755 mL 1.8774 mL 3.7547 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (9.39 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (9.39 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (9.39 mM); Clear solution

    • 4.

      Add each solvent one by one:  corn oil

      Solubility: 16.67 mg/mL (62.59 mM); Clear solution; Need ultrasonic

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [2]

    In cytotoxicity assays, 10,000 cells/well are added to 96 wells plates and incubated overnight, thereafter cells are treated with different concentrations of Honokiol dissolved in dimethylsulphoxide (DMSO). Since Honokiol is not soluble in aqueous solvents, for in vitro studies Honokiol is dissolved in DMSO. To study the possible effect of DMSO on cells, solvent (DMSO) control is used at highest concentration of <0.1%. After 72 h treatment, cells are fixed and cell viability is measured by crystal violet staining (0.05%).

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2]

    For anticancer in vivo studies, the MDA-MB-231 cells (2 million) are injected into mammary fat tissue. Two weeks after the tumor cell injections, palpable tumors are observed in mammary tissues, which is an indication of tumor formation. Then drug treatment either in free form or in nanomicellar forms is given orally at the dose of 40 and 80 mg/kg daily. The drug treatment is continued for 4 weeks, and the tumor volumes and body weights are recorded weekly. After 4 weeks of treatment, animals are sacrificed; final tumor volumes and weights are measured. These tumors are used for western blot and immunohistochemical analysis. For western blot experiments, tumor tissues are stored at −80°C till the analysis is done. For IHC, tumors are fixed in formal saline.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Keywords:

    HonokiolNSC 293100NSC293100NSC-293100AktERKAutophagyHCVPKBProtein kinase BExtracellular signal regulated kinasesHepatitis C virusInhibitorinhibitorinhibit

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    Product Name:
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    Cat. No.:
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