Lactic acid (Synonyms: DL-Lactic acid; E-270)

Name: Lactic acid
Brand: MedChemExpress
SKU: HY-B2227
Rating: 5.0 (52 reviews)

Cat. No.: HY-B2227 Pureza: 85.12%: Ficha de datos
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Lactic acid (DL-Lactic acid) is a hydroxycarboxylic acid receptor 1 (HCAR1) activator and an epigenetic modulator inducing lysine residues lactylation. Lactic acid is a glycolysis end-product, bridging the gap between glycolysis and oxidative phosphorylation. Lactic acid is an oncometabolite and has immune protective role of lactate in anti-tumor immunity. Lactic acid also has antimicrobial activity, which can be used as a food preservative.

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No. CAS : 50-21-5

Tamaño	Stock	Cantidad
100 mg	En stock	Estimated Time of Arrival: December 31
500 mg	En stock	Estimated Time of Arrival: December 31
1 g	En stock	Estimated Time of Arrival: December 31
5 g	En stock	Estimated Time of Arrival: December 31
10 g	En stock	Estimated Time of Arrival: December 31
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Revisión del cliente

Based on 52 publication(s) in Google Scholar

Other Forms of Lactic acid:

Lactic acid, Biotin labeled In-stock
Ammonium lactate (60%w/w in water) In-stock
Lactic acid calcium In-stock
Lactic acid sodium (60% w/w in water) In-stock
Lactic acid potassium In-stock
Lithium 2-hydroxypropanoate In-stock
Lactic acid (Standard) In-stock

52 Publications Citing Use of MCE Lactic acid

RT-PCR

: Lactic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 29:e09477. [Abstract]; Following the direct addition of lactic acid (a final concentration of 100 mM) to the culture medium for 12 h, western blotting, and immunoprecipitation assays revealed a significant increase in the total lactylation and corresponding lysine-312 lactylation levels in ASH2L

: Lactic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar 27:e2413709. [Abstract]; Western blot of UGDH lactylation in chondrocytes with Lactic acid sodium (lactate) (25 mM) treatment for 24 h.

: Lactic acid purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Apr:81:103553. [Abstract]; Immunoblot and quantification analyses of NFS1 in HCC cells incubated with 0, 5, or 10 mM lactate after being subjected to either a 47 °C or 37 °C water bath.

: Lactic acid purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Mar 20;16(1):193. [Abstract]; Lactate sodium (5-15 mM; 24 h) results in increased H4K12la (red) levels in CCSCs compared to the control group.

: Lactic acid purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Feb 28;16(1):144. [Abstract]; Lactate (25 mM) significantly increases the mRNA and protein expression of FBXO33.

: Lactic acid purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Feb 28;16(1):144. [Abstract]; Co-IP detected the effect of lactate on YY1 protein lactylation modification.

: Lactic acid purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Mar 5;43(1):68. [Abstract]; Lactate sodium increases IGF-1R levels in PI-resistant KAS-6/1 and U266 cells.

: Lactic acid purchased from MedChemExpress. Usage Cited in: Cell Res. 2023 Sep;33(9):679-698. [Abstract]; Lactate (NALA) (300 mM; 24 h) significantly upregulates α-MHC K1897 lactylation in H9c2 cells.

: Lactic acid purchased from MedChemExpress. Usage Cited in: J Ginseng Res. 2024 May;48(3):298-309.; The expression level of METTL3, YTHDF1, YTHDF2, and YTHDC1 in NB4-R1 and NB4-R2 cells treated with 20 μM oxamate or 20 μM lactate or 1 μM ATRA for 48 h was detected via qRT-PCR.

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Ver todos los productos específicos de isoformas Hydroxycarboxylic Acid Receptor (HCAR):

Ver todas las isoformas

HCAR1 HCAR2

Ver todos los productos específicos de isoformas Endogenous Metabolite:

Ver todas las isoformas

Human Endogenous Metabolite Microbial Metabolite

Actividad biológica
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Referencias
Revisión del cliente

Descripciòn

Lactic acid (DL-Lactic acid) is a hydroxycarboxylic acid receptor 1 (HCAR1) activator and an epigenetic modulator inducing lysine residues lactylation. Lactic acid is a glycolysis end-product, bridging the gap between glycolysis and oxidative phosphorylation. Lactic acid is an oncometabolite and has immune protective role of lactate in anti-tumor immunity^[1]^[2]. Lactic acid also has antimicrobial activity, which can be used as a food preservative^[3].

IC₅₀ & Target^[1]^[2]

HCAR1

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	26.6 3 Compound: Lactic acid	Antiproliferative activity against human A549 cells after 48 hrs by MTT assay Antiproliferative activity against human A549 cells after 48 hrs by MTT assay	[PMID: 28710964]
A549	IC₅₀	26.61 2 Compound: Lactic acid	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 25299679]
MRC5	IC₅₀	23.93 2 Compound: Lactic acid	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 25299679]
A549	IC₅₀	26.61 2 Compound: Lactic acid	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 25299679]
MRC5	IC₅₀	23.93 2 Compound: Lactic acid	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 25299679]
MRC5	IC₅₀	23.93 3 Compound: Lactic acid	Cytotoxicity against human MRC5 cells after 48 hrs by MTT assay Cytotoxicity against human MRC5 cells after 48 hrs by MTT assay	[PMID: 28710964]

In Vitro

Lactic acid (20 mM; 24, 48 h) results in a significant decrease in cell proliferation and migration, acting and switching cell metabolism toward oxidative phosphorylation^[1].
Lactic acid (20 mM; 24 h) shows an increase in the protein and mRNA expression levels of MCT1 and HCAR1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Uveal melanoma 92.1 cells
Concentration:	20 mM
Incubation Time:	24, 48 h
Result:	Inhibited cell proliferation and migration.

Western Blot Analysis^[1]

Cell Line:	Uveal melanoma cells
Concentration:	20 mM
Incubation Time:	24 h
Result:	Showed an increase in the protein expression levels of MCT1 and HCAR1

RT-PCR^[1]

Cell Line:	Uveal melanoma cells
Concentration:	20 mM
Incubation Time:	24 h
Result:	Induced a significant increase in mRNA expression levels of SLC16A1 and HCAR1.

In Vivo

Lactic acid (1.6 g/kg; pH 7.4; subcutaneous; daily from Day 8 to 30 days) shows CD8+ T cell-dependent tumor growth inhibition in MC38 colon cancer model^[2].
Note: The pH of the working solution needs to be adjusted to around 7.4 before administration to animals.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female C57BL/6 mice with MC38 colon cancer cells^[2]
Dosage:	1.6 g/kg
Administration:	Subcutaneous; daily from Day 8 to 30 days
Result:	Significantly suppressed tumor growth.

Peso molecular

90.08

Fòrmula

C₃H₆O₃

No. CAS

50-21-5

Appearance

Liquid (Density: 1.209 g/cm³)

Color

Colorless to light yellow

SMILES

CC(O)C(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

H₂O : ≥ 200 mg/mL (2220.25 mM)

DMSO : ≥ 200 mg/mL (2220.25 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	11.1012 mL	55.5062 mL	111.0124 mL
5 mM	2.2202 mL	11.1012 mL	22.2025 mL
10 mM	1.1101 mL	5.5506 mL	11.1012 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (55.51 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (55.51 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (55.51 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Pureza y Documentación

Purity: 85.12%

Select Batch:

Ficha de datos (278 KB) SDS (476 KB)

COA (248 KB) HNMR (211 KB) RP-HPLC (254 KB) MS (83 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Lucia Longhitano, et al. Lactate Rewrites the Metabolic Reprogramming of Uveal Melanoma Cells and Induces Quiescence Phenotype. Int J Mol Sci. 2022 Dec 20;24(1):24. [Content Brief]

[2]. Qiang Feng, et al. Lactate increases stemness of CD8 + T cells to augment anti-tumor immunity. Nat Commun. 2022 Sep 6;13(1):4981. [Content Brief]

[3]. Sallam KI. Antimicrobial and antioxidant effects of sodium acetate, sodium lactate, and sodium citrate in refrigerated sliced salmon. Food Control. 2007 May;18(5):566-575. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	11.1012 mL	55.5062 mL	111.0124 mL	277.5311 mL
	5 mM	2.2202 mL	11.1012 mL	22.2025 mL	55.5062 mL
	10 mM	1.1101 mL	5.5506 mL	11.1012 mL	27.7531 mL
	15 mM	0.7401 mL	3.7004 mL	7.4008 mL	18.5021 mL
	20 mM	0.5551 mL	2.7753 mL	5.5506 mL	13.8766 mL
	25 mM	0.4440 mL	2.2202 mL	4.4405 mL	11.1012 mL
	30 mM	0.3700 mL	1.8502 mL	3.7004 mL	9.2510 mL
	40 mM	0.2775 mL	1.3877 mL	2.7753 mL	6.9383 mL
	50 mM	0.2220 mL	1.1101 mL	2.2202 mL	5.5506 mL
	60 mM	0.1850 mL	0.9251 mL	1.8502 mL	4.6255 mL
	80 mM	0.1388 mL	0.6938 mL	1.3877 mL	3.4691 mL
	100 mM	0.1110 mL	0.5551 mL	1.1101 mL	2.7753 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Lactic acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Lactic acid (Synonyms: DL-Lactic acid; E-270)

Revisión del cliente

Other Forms of Lactic acid:

Lactic acid Related Antibodies

52 Publications Citing Use of MCE Lactic acid

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Hydroxycarboxylic Acid Receptor (HCAR):

Ver todos los productos específicos de isoformas Endogenous Metabolite:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

Lactic acid Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

Lactic acid Related Classifications

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Productos vistos recientemente:

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