Phenylacetylglycine

Name: Phenylacetylglycine
Brand: MedChemExpress
SKU: HY-W015061
Rating: 4.5 (1 reviews)

Cat. No.: HY-W015061 Pureté: 99.42%: Fiche technique
COA

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Instruction de manipulation
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Phenylacetylglycine is a gut microbial metabolite that can activate β2AR. Phenylacetylglycine protects against cardiac injury caused by ischemia/reperfusion.

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Phenylacetylglycine Chemical Structure

CAS No. : 500-98-1

Size	Stock	Quantité

Échantillon gratuit (0.1 - 0.2 mg)	Appliquer maintenant
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
250 mg	En stock	Estimated Time of Arrival: December 31
500 mg	Obtenir un devis
1 g	Obtenir un devis

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Based on 1 publication(s) in Google Scholar

Other Forms of Phenylacetylglycine:

Phenylacetylglycine (Standard) In-stock
N-(Phenylacetyl-d₅)glycine Obtenir un devis

1 Publications Citing Use of MCE Phenylacetylglycine

•Adv Sci (Weinh). 2024 May;11(20):e2306297. [Abstract]

Recommandations en vedette

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

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α adrenergic receptor β adrenergic receptor

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Human Endogenous Metabolite Microbial Metabolite

Activité biologique
Pureté et documentation
Références
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Description

Phenylacetylglycine is a gut microbial metabolite that can activate β2AR. Phenylacetylglycine protects against cardiac injury caused by ischemia/reperfusion^[1].

IC₅₀ & Target^[1]

β2 adrenoceptor

Microbial Metabolite

Human Endogenous Metabolite

In Vitro

Phenylacetylglycine (10-100 μM; 30 min before H/R injury) reduces disreoxygenation (H/R) injury-induced apoptosis and activates Gαi and Gαs signaling in neonatal mouse cardiomyocytes (NMCMs)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:	Neonatal mouse cardiomyocytes (NMCMs)
Concentration:	10, 33 and 100 μM
Incubation Time:	Half an hour before H/R injury
Result:	Inhibited disreoxygenation injury induced apoptosis.

Western Blot Analysis^[1]

Cell Line:	Neonatal mouse cardiomyocytes (NMCMs)
Concentration:	10, 33 and 100 μM
Incubation Time:	Half an hour before H/R injury
Result:	Significantly decreased the ratio of Bax/Bcl2 and cleaved-caspase 3 expression. Enhanced p-PI3K protein expression. cAMP levels were increased in the early stage and then gradually decreased.

Masse moléculaire

193.20

Formule

C₁₀H₁₁NO₃

CAS No.

500-98-1

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CNC(CC1=CC=CC=C1)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Endogenous metabolite

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (517.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.1760 mL	25.8799 mL	51.7598 mL
5 mM	1.0352 mL	5.1760 mL	10.3520 mL
10 mM	0.5176 mL	2.5880 mL	5.1760 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.83 mg/mL (4.30 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.83 mg/mL (4.30 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.83 mg/mL (4.30 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 99.42%

Select Batch:

Fiche technique (272 KB) SDS (392 KB)

COA (244 KB) LCMS (88 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Xu X, et al. The gut microbial metabolite phenylacetylglycine protects against cardiac injury caused by ischemia/reperfusion through activating β2AR. Arch Biochem Biophys. 2021 Jan 15;697:108720. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.1760 mL	25.8799 mL	51.7598 mL	129.3996 mL
	5 mM	1.0352 mL	5.1760 mL	10.3520 mL	25.8799 mL
	10 mM	0.5176 mL	2.5880 mL	5.1760 mL	12.9400 mL
	15 mM	0.3451 mL	1.7253 mL	3.4507 mL	8.6266 mL
	20 mM	0.2588 mL	1.2940 mL	2.5880 mL	6.4700 mL
	25 mM	0.2070 mL	1.0352 mL	2.0704 mL	5.1760 mL
	30 mM	0.1725 mL	0.8627 mL	1.7253 mL	4.3133 mL
	40 mM	0.1294 mL	0.6470 mL	1.2940 mL	3.2350 mL
	50 mM	0.1035 mL	0.5176 mL	1.0352 mL	2.5880 mL
	60 mM	0.0863 mL	0.4313 mL	0.8627 mL	2.1567 mL
	80 mM	0.0647 mL	0.3235 mL	0.6470 mL	1.6175 mL
	100 mM	0.0518 mL	0.2588 mL	0.5176 mL	1.2940 mL