Tibulizumab  (Synonyms: LY 3090106)

Tibulizumab (LY 3090106) is a tetravalent bispecific monoclonal antibody targeting B-cell activating factor (BAFF) and IL-17A with Kd values of 60 pM and 14 pM, respectively. Tibulizumab can be used for autoimmune disease research^[1].

IgG4-kappa-[scFv-heavy-kappa]2

Human IgG4 (S228P) kappa, Isotype Control

Human

Tibulizumab (LY 3090106) binds to mouse BAFF with a K_D of 340 nM and does not bind to mouse IL-17. Tibulizumab binds to cynomolgus BAFF and cynomolgus IL-17 with K_D of 26.8 pM and 19 pM, respectively^[1].
In HT-29 cells, Tibulizumab antagonizes the IL-17-induced secretion of CXCL1 in a concentration-dependent manner (IC₅₀ of 2.00 nM). In T1165 cells, Tibulizumab blocks BAFF-induced proliferation in a concentration-dependent manner (IC₅₀ of 0.064 nM). Tibulizumab simultaneously binds and blocks both BAFF and IL-17^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tibulizumab (LY 3090106; 660 µg/mouse, i.p; 66 µg/mouse, s.c) effectively antagonizes the biological effects induced by human BAFF and IL-17 in the mouse^[1].
In cynomolgus monkey, Tibulizumab (i.v, 0.3, 1, 5, or 20 mg/kg; or s.c, 5 mg/kg) suppresses B cell development and survival and remains functionally intact in circulation, with a prolonged half-life^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

10673  [NCBI] & 16171  [NCBI]

Q9Y275 & Q62386

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

201.43 kDa

1849636-24-3

Liquid

Colorless to light yellow

[Tibulizumab]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  IgG4-kappa-[scFv-heavy-kappa]2

• 
  Immobilized BAFF/TNFSF13B Protein, Human (CHO, HY-P7003) can bind Tibulizumab. The EC₅₀ for this effect is 36.68 ng/mL.
• 
  Immobilized IL-17A Protein, Human (HEK293, His, HY-P70527) can bind Tibulizumab. The EC₅₀ for this effect is 19.5 ng/mL.

• [1]. Robert J Benschop, et al. Development of tibulizumab, a tetravalent bispecific antibody targeting BAFF and IL-17A for the treatment of autoimmune disease. MAbs. 2019 Aug/Sep;11(6):1175-1190.  [Content Brief]