Anabasine (Synonyms: (S)-Anabasine; (+)-Anabasine)

Name: Anabasine
Brand: MedChemExpress
SKU: HY-B1532
Rating: 4.5 (0 reviews)

Cat. No.: HY-B1532 Pureza: 99.02%: Ficha de datos
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Anabasine ((S)-Anabasine) is an alkaloid that found as a minor component in tobacco (Nicotiana). Anabasine is a botanical pesticide nicotine, acts as a full agonist of nicotinic acetylcholine receptors (nAChRs). Anabasine induces depolarization of TE671 cells endogenously expressing human fetal muscle-type nAChRs (EC₅₀=0.7 μM).

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Anabasine Estructura química

No. CAS : 494-52-0

Tamaño	Stock	Cantidad
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Liquid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Other Forms of Anabasine:

(±) Anabasine In-stock
Anabasine hydrochloride Obtener un presupuesto
Anabasine (Standard) Obtener un presupuesto

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Protocolo
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Descripciòn

IC₅₀ & Target

nicotinic receptor^[1]

In Vivo

Anabasine significantly reverses the impairment at the 0.2 mg/kg (p<0.05) and 2 mg/kg doses (p<0.025). Anabasine does not have any significant effects on response latency when administered alone. The 0.06 mg/kg Anabasine dose, in fact, significantly (p<0.05) exacerbates the dizocilpine-induced impairment. None of these Anabasine doses affects choice accuracy on their own. Individual dose comparisons show that the 0.06 mg/kg Anabasine dose plus dizocilpine (6.7±2.6) causes a significant (p<0.05) increase in non-response trials compare with dizocilpine alone (2.1±0.8)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

162.24

Fòrmula

C₁₀H₁₄N₂

No. CAS

494-52-0

Appearance

Liquid (Density: 1.0455 g/cm³)

Color

Colorless to light yellow

SMILES

C1([C@H]2NCCCC2)=CC=CN=C1

Structure Classification

Initial Source

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (616.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.1637 mL	30.8185 mL	61.6371 mL
5 mM	1.2327 mL	6.1637 mL	12.3274 mL
10 mM	0.6164 mL	3.0819 mL	6.1637 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (15.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.02%

Select Batch:

Ficha de datos (274 KB) SDS (644 KB)

COA (249 KB) HNMR (234 KB) RP-HPLC (252 KB) LCMS (94 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Levin ED, et al. Effects of tobacco smoke constituents, anabasine and anatabine, on memory and attention in female rats. J Psychopharmacol. 2014 Oct;28(10):915-22. [Content Brief]

[2]. Benedict T Green, et al. Actions of piperidine alkaloid teratogens at fetal nicotinic acetylcholine receptors. Neurotoxicol Teratol. May-Jun 2010;32(3):383-90. [Content Brief]

Administraciòn de animales
^[1]

Adult (age range 4 to 16 months) female Sprague-Dawley rats are used in this study. One group of rats (N=12) is trained on the radial arm maze test of working and reference memory and undergo tests of an acute dose-response of Anabasine (0.02, 0.2, 1 and 2 mg/kg). Then, two doses of Anabasine (0.2 and 2 mg/kg) are tested alone or in combination with the glutamate NMDA antagonist dizocilpine (0.05 mg/kg). The saline vehicle is used as vehicle control and dizocilpine alone is used as an impaired control. All conditions are given to each rat in a repeated measures counterbalanced design^[1].

MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.

Referencias

[1]. Levin ED, et al. Effects of tobacco smoke constituents, anabasine and anatabine, on memory and attention in female rats. J Psychopharmacol. 2014 Oct;28(10):915-22. [Content Brief]

[2]. Benedict T Green, et al. Actions of piperidine alkaloid teratogens at fetal nicotinic acetylcholine receptors. Neurotoxicol Teratol. May-Jun 2010;32(3):383-90. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.1637 mL	30.8185 mL	61.6371 mL	154.0927 mL
	5 mM	1.2327 mL	6.1637 mL	12.3274 mL	30.8185 mL
	10 mM	0.6164 mL	3.0819 mL	6.1637 mL	15.4093 mL
	15 mM	0.4109 mL	2.0546 mL	4.1091 mL	10.2728 mL
	20 mM	0.3082 mL	1.5409 mL	3.0819 mL	7.7046 mL
	25 mM	0.2465 mL	1.2327 mL	2.4655 mL	6.1637 mL
	30 mM	0.2055 mL	1.0273 mL	2.0546 mL	5.1364 mL
	40 mM	0.1541 mL	0.7705 mL	1.5409 mL	3.8523 mL
	50 mM	0.1233 mL	0.6164 mL	1.2327 mL	3.0819 mL
	60 mM	0.1027 mL	0.5136 mL	1.0273 mL	2.5682 mL
	80 mM	0.0770 mL	0.3852 mL	0.7705 mL	1.9262 mL
	100 mM	0.0616 mL	0.3082 mL	0.6164 mL	1.5409 mL

Anabasine Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anabasine494-52-0(S)-Anabasine (+)-AnabasinenAChREnvironmental PollutantsNicotinic acetylcholine receptorsEnvironmental ContaminantsalkaloidTE 671pesticidemuscle-typeInhibitorinhibitorinhibit

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Anabasine (Synonyms: (S)-Anabasine; (+)-Anabasine)

Revisión del cliente

Other Forms of Anabasine:

Anabasine Related Antibodies

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Actividad biológica

Protocolo

Pureza y Documentación

Referencias

Revisión del cliente

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

Anabasine Related Classifications

Keywords:

Productos vistos recientemente:

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