Talfirastide
Based on 17 publication(s) in Google Scholar
Angiotensin 1-7 (Ang-(1-7)) is an endogenous heptapeptide from the renin-angiotensin system (RAS) with a cardioprotective role due to its anti-inflammatory and anti-fibrotic activities in cardiac cells. Angiotensin 1-7 inhibits purified canine ACE activity (IC50=0.65 μM). Angiotensin 1-7 acts as a local synergistic modulator of kinin-induced vasodilation by inhibiting ACE and releasing nitric oxide. Angiotensin 1-7 blocks Ang II-induced smooth muscle cell proliferation and hypertrophy and shows antiangiogenic and growth-inhibitory effects on the endothelium. Angiotensin 1-7 shows anti-inflammatory activity .
For research use only. We do not sell to patients.
- Purity: 99.90%
- CAS No.: 51833-78-4
- Formula: C41H62N12O11
- Molecular Weight:899.00
-
Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocol
- Purity & Documentation
- References
-
Help & FAQs
-
GPCR/G Protein Compound Library
HY-L006
-
Immunology/Inflammation Compound Library
HY-L007
-
Metabolism/Protease Compound Library
HY-L012
-
Anti-Cancer Compound Library
HY-L025
-
Clinical Compound Library
HY-L026
-
Peptidomimetic Library
HY-L033
-
Drug Repurposing Compound Library
HY-L035
-
Anti-Cardiovascular Disease Compound Library
HY-L046
-
Endocrinology Compound Library
HY-L047
-
Orally Active Compound Library
HY-L061
-
Neurotransmitter Receptor Compound Library
HY-L062
-
Neuroprotective Compound Library
HY-L070
-
Angiogenesis-Related Compound Library
HY-L088
-
Rare Diseases Drug Library
HY-L102
-
Peptide Library
HY-L105
-
Human Metabolite Library
HY-L123
-
Anti-Prostate Cancer Compound Library
HY-L124
-
Heterocyclic Compound Library
HY-L138
-
Antihypertensive Compound Library
HY-L145
-
Protease Inhibitor Library
HY-L147
-
Membrane Protein-targeted Compound Library
HY-L149
-
Membrane Receptor-targeted Compound Library
HY-L150
-
Multi-Target Compound Library
HY-L176
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Drug Repurposing Compound Library Plus
HY-L035P
-
Clinical Compound Library Plus
HY-L026P
-
Peptide Compound Library
HY-L105S
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
-
Mass Spectrometry Human Metabolite Library
HY-L215
-
Natural Product Diversity Scaffold Library
HY-L245
-
Lactylation Compound Library
HY-L249
Publications Citing Use of MedChemExpress (MCE) Talfirastide
More- Proc Natl Acad Sci U S A. 2025 Dec 9;122(49):e2515641122. [Abstract]
- Chin Chem Lett. 2023 Mar;34(3):107522. [Abstract]
- Phytomedicine. 2025 Oct 4:148:157371. [Abstract]
- Cell Biosci. 2023 Feb 4;13(1):23. [Abstract]
- Acta Physiol. 2026 Apr;242(4):e70200. [Abstract]
- Chem Biol Interact. 2025 Jun 2:111596. [Abstract]
- Biol Proced Online. 2022 Oct 25;24(1):15. [Abstract]
- Front Cell Dev Biol. 2021 Jun 11:9:659809. [Abstract]
- J Inflamm Res. 2024 Jan 24:17:469-485. [Abstract]
- Sci Rep. 2025 Nov 25. [Abstract]
- Front Biosci (Landmark Ed). 2024 Sep 24;29(9):334. [Abstract]
- BMC Pulm Med. 2023 Nov 2;23(1):422. [Abstract]
- Endokrynol Pol. 2023;74(5):544-552. [Abstract]
- Int J Clin Exp Med. 2019;12(5):4773-4780.
- bioRxiv. 2026 Mar 25.
- SSRN. 2025 Feb 28.
- Research Square Preprint. 2024 Feb 19.
Customer Validation & Images
-
Cell Imaging/Staining
-
WB
-
Flow Cytometry
-
Flow Cytometry
-
RT-PCR
All Angiotensin Receptor Isoforms
MoreAll Endogenous Metabolite Isoforms
More
Biological Activity
|
AT1 Receptor |
Angiotensin 1-7 (Ang-(1-7)) inhibits cultured vascular smooth muscle cell growth, whereas equal molar concentration of Ang II stimulates cell growth[2].
Angiotensin 1-7 (Ang 1-7) abrogates the methylglyoxal-modified albumin (MGA)-stimulated myofibroblast phenotype by inhibiting the chronic stimulation of the TGF-β-ERK pathway in NRK-52E cells[4].
Angiotensin 1-7 signals through the Mas receptor ( MasR) in opposition to Ang II/angiotensin II type 1 receptor (AT1R), promoting anti-inflammatory,vasodilatory, and neuroprotective effects[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
-
CAS No. 51833-78-4
-
Appearance Solid
-
Molecular Weight 899.00
-
Formula C41H62N12O11
-
Color White to off-white
-
Synonyms
TXA127; Angiotensin (1-7); Ang-(1-7)
-
Sequence
Asp-Arg-Val-Tyr-Ile-His-Pro
-
Sequence Shortening
DRVYIHP
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (17)
-
Journal Impact Factor
-
Most Recent
-
Proc Natl Acad Sci U S A
Ace2 safeguards embryonic hematopoietic stem and progenitor cell production by restraining Nlrp3-mediated pyroptosis. [Abstract]2025 Dec 9;122(49):e2515641122. PMID: 41348733 -
Chin Chem Lett
Nanokit coupled electrospray ionization mass spectrometry for analysis of angiotensin converting enzyme 2 activity in single living cell. [Abstract]2023 Mar;34(3):107522. PMID: 35602918 -
Phytomedicine
Baicalin and ginsenoside Rb1 suppress the activation of cardiac fibroblasts via regulating the GRK2/AT1R/MasR network. [Abstract]2025 Oct 4:148:157371. PMID: 41110355 -
Cell Biosci
Ang-(1-7)/MasR axis promotes functional recovery after spinal cord injury by regulating microglia/macrophage polarization. [Abstract]2023 Feb 4;13(1):23. PMID: 36739421
Talfirastide purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2023 Feb 4;13(1):23. [Abstract]
Ang-(1–7) (Talfirastide, 10−6 mol/L, 24 h) regulates the polarization of macrophages towards the M2 phenotype. Double-staining for F4/80 (green)/iNOS (red) or F4/80 (green)/Arg-1 (red) in each group of macrophages (scale bar: 50 μm, n = 5).
Talfirastide purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2023 Feb 4;13(1):23. [Abstract]
(Talfirastide, 10−6 mol/L). Representative immunoblots and quantification of macrophages in each group showing the expression of iNOS and Arg-1 after treatment for 24 h (n = 3).
Talfirastide purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2023 Feb 4;13(1):23. [Abstract]
Talfirastide (10−6 mol/L, 24h). The ratio of M1 macrophage phenotype-related surface markers, CD86/CD11b, were determined by flow cytometry for each group (n = 3).
Talfirastide purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2023 Feb 4;13(1):23. [Abstract]
Talfirastide (10−6 mol/L, 24h). The ratio of M2 macrophage phenotype-related surface markers, CD206/ CD11b, were determined by flow cytometry for each group (n = 3).
Talfirastide purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2023 Feb 4;13(1):23. [Abstract]
Ang-(1–7) (Talfirastide, 10−6 mol/L, 24h) inhibits inflammation response by modulating the expression of inflammatory cytokines in BMDMs. Quantitative RT-qPCR analysis of macrophages showing mRNA expression of IL-1β, TNF-α, IL-10, IL-4 after treatment for 24 h. GAPDH: loading control. Data are expressed as fold change compared to the control group (n = 3).
Talfirastide purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2023 Feb 4;13(1):23. [Abstract]
Ang-(1–7)(Talfirastide, 10−6 mol/L, 24h) inhibits inflammation response by modulating the expression of inflammatory cytokines. Representative immunoblots and quantification showing protein expression of IL-1β and TNF-α in the macrophages after treatment for 24 h.
Talfirastide purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2023 Feb 4;13(1):23. [Abstract]
Ang-(1–7) (Talfirastide; administered immediately by intrathecal injection; 10 μL; 500 ng) improved the motor function of rats after SCI. a Basso, Beattie and Bresnahan (BBB) scores (n = 6) and Inclined plane test scores (n = 6).
Talfirastide purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2023 Feb 4;13(1):23. [Abstract]
Continuous sections of the spinal cord by HE staining were obtained on the 28th day post-surgery, and the remaining neurons in the SCI group, SCI + Ang-(1–7) groups, and SCI + Ang-(1–7) + A779 groups at the same distance from the epicenter were displayed. (scale bar: 100 μm and 40 μm, n = 3).
-
Acta Physiol
Angiotensin-(1-7) Alleviates Isoproterenol-Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor. [Abstract]2026 Apr;242(4):e70200. PMID: 41886752 -
Chem Biol Interact
Mechanism of ferroptosis in hypoxia-induced pulmonary vascular remodeling in hypoxia pulmonary hypertension: a study based on the ACE2-Ang-(1-7)-Mas axis. [Abstract]2025 Jun 2:111596. PMID: 40466901 -
Biol Proced Online
Angiotensin-converting enzyme 2 identifies immuno-hot tumors suggesting angiotensin-(1-7) as a sensitizer for chemotherapy and immunotherapy in breast cancer. [Abstract]2022 Oct 25;24(1):15. PMID: 36284262 -
Front Cell Dev Biol
2021 Jun 11:9:659809. PMID: 34178985 -
J Inflamm Res
Angiotensin-(1-7) Modulates the Warburg Effect to Alleviate Inflammation in LPS-Induced Macrophages and Septic Mice. [Abstract]2024 Jan 24:17:469-485. PMID: 38282712 -
Sci Rep
Ginsenoside Rg1 alleviates HG-induced autophagy of retinal microvascular endothelial cells by activating ACE2/Ang1-7/Mas in vitro. [Abstract]2025 Nov 25. PMID: 41290961 -
Front Biosci (Landmark Ed)
ACE2 Alleviates Endoplasmic Reticulum Stress and Protects against Pyroptosis by Regulating Ang1-7/Mas in Ventilator-Induced Lung Injury. [Abstract]2024 Sep 24;29(9):334. PMID: 39344337 -
BMC Pulm Med
Angiotensin-(1-7) suppresses airway inflammation and airway remodeling via inhibiting ATG5 in allergic asthma. [Abstract]2023 Nov 2;23(1):422. PMID: 37919667 -
Endokrynol Pol
Angiotensin (1-7) reverses glucose-induced islet β cell dedifferentiation by Wnt/β-catenin/FoxO1 signalling pathway. [Abstract]2023;74(5):544-552. PMID: 37779373 -
-
-
-
Solvent & Solubility
H2O : 25 mg/mL (27.81 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (111.23 mM); Clear solution; Need ultrasonic
Protocol
Competition assays using purified canine ACE are determined using a fixed concentration of the substrate Hip-His-Leu (1 mM) and varying the concentrations of the competing agents [Lisinopril (0.1 to 100 nM), Angiotensin (1-7) (10 nM to 10 μM), or Sar1, Thr8-Ang II (10 nM to 10 μM)]. Inhibitory constants (IC50) are determined from the respective competition curves. To study the effect of Angiotensin (1-7) on BK metabolism in intact coronary rings, 125I-[Tyr0]-BK (final concentration of 1 nM) is added to the tubes containing three rings preincubated with 1 mL Krebs' buffer and aerated with 95% O2 and 5% CO2 at 37°C. Lisinopril (2 μM), Angiotensin (1-7) (2 μM), or Krebs' buffer as control are added to the rings 10 minutes before addition of the radiolabeled BK. Aliquots of the incubation medium are removed at 5, 10, and 20 minutes and diluted with 1% HFBA to inhibit peptidase activity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
500 μM Methylglyoxal is incubated with 100 μM BSA dissolved in phosphate buffered saline (PBS) for 24 hours, then washed on 10 kDa filters to remove excess methyl glyoxal, reconstituted with DMEM/F12 serum free media and passed through a 0.2 μmicron filter. TGF-β (5 ng/mL) is prepared to treat cells in a subset of experiments. Cells are co-treated with one or combinations of the following: Angiotensin (1-7) (100 nM), D-Ala7-Ang-(1-7) (10 μM), ERK1/2 kinase inhibitor, PD 98059 (1 μM), TGF-β receptor kinase inhibitor; SB525334 (1 μM), the AT1 receptor antagonist Losartan (1 μM), the renin inhibitor Aliskerin (1 μM) and the ACE inhibitor Lisinopril (1 μM)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[3]
Male and female BALB/c mice (1:1 ratio, 6-10 weeks old, mean weight 20 g.) are used. Angiotensin fragment 1-7 acetate salt hydrate (Ang 1-7) is dissolved in 0.9% saline (vehicle) at 1 mg/mL and stored at -80°C. Various doses (0.01, 0.06, 0.1, 0.3 and 1 mg/kg) are freshly prepared from the stock each day of the experiment, and administered to mice by daily intra-peritoneal (i.p) injections in a volume of 500 μL per injection, either before (prophylactic approach) or after (treatment approach) DSS treatment. A779 (MAS-1 R antagonist) is similarly dissolved in distilled water at 1 mg/mL and stored at -80°C. A freshly prepared dose of 1 mg/kg is administered to a second group of mice by daily i.p injections in a volume of 500 μL daily (for 4 days) along with colitis induction (prophylactic approach). A third group of mice receive DSS containing water and daily i.p injections of 0.9% saline (vehicle). The fourth group receive DSS containing water along with daily i.p injections with Dexamethasone (DEX) at doses of 0.01-1.0 mg/kg or its vehicle (0.9% saline) (prophylactic approach).
Rats[4]
Twenty six ovariectomized female Wistar rats weighing 200±20 g are used. Angiotensin (1-7) is administered intravenously by a microsyringe pump at two different continuous doses of 100 and 300 ng/kg/min after antagonist/saline infusion. Each dose is infused for 15 min; and MAP, RPP, and RBF are recorded during Angiotensin (1-7) infusion and the last 3-5 min of each dose measured as “response to Angiotensin (1-7) infusion”. During Angiotensin (1-7) infusion, RPP is sustained at pre-Ang1-7 infusion levels via an adjustable aortic clamp.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
-
Data Sheet (291 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
[1]. Gómez-Mendoza DP, et al. Angiotensin-(1-7) oral treatment after experimental myocardial infarction leads to downregulation of CXCR4. J Proteomics. 2019;208:103486. [Content Brief]
[2]. Li P, et al. Angiotensin-(1-7) augments bradykinin-induced vasodilation by competing with ACE and releasing nitric oxide. Hypertension. 1997 Jan;29(1 Pt 2):394-400. [Content Brief]
[3]. Khajah MA, et al. Anti-Inflammatory Action of Angiotensin 1-7 in Experimental Colitis. PLoS One. 2016 Mar 10;11(3):e0150861. [Content Brief]
[4]. Alzayadneh EM, et al. Angiotensin-(1-7) abolishes AGE-induced cellular hypertrophy and myofibroblast transformation via inhibition of ERK1/2. Cell Signal. 2014 Sep 19. pii: S0898-6568(14)00314-3. [Content Brief]
[5]. Janatpour ZC, et al. Subcutaneous Administration of Angiotensin-(1-7) Improves Recovery after Traumatic Brain Injury in Mice. J Neurotrauma. 2019;36(22):3115-3131. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.1123 mL | 5.5617 mL | 11.1235 mL | 27.8087 mL |
| 5 mM | 0.2225 mL | 1.1123 mL | 2.2247 mL | 5.5617 mL | |
| 10 mM | 0.1112 mL | 0.5562 mL | 1.1123 mL | 2.7809 mL | |
| 15 mM | 0.0742 mL | 0.3708 mL | 0.7416 mL | 1.8539 mL | |
| 20 mM | 0.0556 mL | 0.2781 mL | 0.5562 mL | 1.3904 mL | |
| 25 mM | 0.0445 mL | 0.2225 mL | 0.4449 mL | 1.1123 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Powered by Bioz