デシタビン (Synonyms: Decitabine; 5-Aza-2′-Deoxycytidine; 5-AZA-CdR)

Name: Decitabine
Brand: MedChemExpress
SKU: HY-A0004
Rating: 5.0 (168 reviews)

製品番号: HY-A0004 純度: 99.87%: Data Sheet SDS COA 取扱説明書
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Technical Support

Decitabine (NSC 127716) is an orally active deoxycytidine analogue antimetabolite and DNA methyltransferase inhibitor. Decitabine incorporates into DNA in place of cytosine can covalently trap DNA methyltransferase to DNA causing irreversible inhibition of the enzyme. Decitabine induces cell G2/M arrest and cell Apoptosis. Decitabine has potent anticancer activity.

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CAS 番号 : 2353-33-5

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	在庫あり	Estimated Time of Arrival: December 31
Solid
50 mg	$30	在庫あり	Estimated Time of Arrival: December 31
100 mg	$50	在庫あり	Estimated Time of Arrival: December 31
500 mg	$150	在庫あり	Estimated Time of Arrival: December 31
1 g	$250	在庫あり	Estimated Time of Arrival: December 31
2 g	$450	在庫あり	Estimated Time of Arrival: December 31
5 g		お問い合わせ
10 g		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 168 publication(s) in Google Scholar

Other Forms of Decitabine:

Decitabine (Standard) 在庫あり
Decitabine-¹³C₅ お問い合わせ
Decitabine-¹⁵N₄ お問い合わせ
Decitabine methanesulfonate お問い合わせ

顧客検証

RT-PCR

Cell Proliferation/Viability Assay

: Decitabine purchased from MedChemExpress. Usage Cited in: Cell. 2024 Apr 25;187(9):2288-2304.e27. [Abstract]; SLC6A6 mRNA levels in the indicated cells treated with decitabine (1 μM for 3 days).

: Decitabine purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 29;2022:2371807. [Abstract]; The toxicity of decitabine to Y79 and WERI-RB-1 cells is detected by CCK-8 assay at different concentrations of Decitabine.

: Decitabine purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 29;2022:2371807. [Abstract]; The mRNA expression of GSDME is detected using RT-PCR after treatment with different concentrations of Decitabine for 3 days (the cells were centrifuged, and decitabine is replaced every day).

: Decitabine purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 29;2022:2371807. [Abstract]; The protein level of GSDME in cells is quantitatively analysed by western blot in Y79 and WERI-RB-1 cells after Decitabine (2 nM; 3 days) treatment.

: Decitabine purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 29;2022:2371807. [Abstract]; The protein level of GSDME in cells is detected using immunofluorescence in Y79 and WERI-RB-1 cells after Decitabine (2 nM; 3 days) treatment.

: Decitabine purchased from MedChemExpress. Usage Cited in: Prog Neurobiol. 2021 Jun:201:102028. [Abstract]; The TF changes induced by DNMT3L in these cells are not affected with Decitabine (10 µM; for 24 hours) treatment in most genes tested, except NEUROD2 and NEUROG2 as shown by qRTPCR.

: Decitabine purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2021 Jul;54(7):e13072. [Abstract]; The indicated HSCs are subjected to DNMTs inhibitor (5‐azadC, 2 μM), G9a inhibitor (UNC0642, 2 μM) and pan‐HDAC inhibitor (ITF‐2357, 100 nM) for 48 h, or EZH2 inhibitor (EPZ‐6438, 10 μM) for 72h. KLF14 expression is measured by RT‐qPCR.

: Decitabine purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2021 Jul;54(7):e13072. [Abstract]; The indicated HSCs are subjected to DNMTs inhibitor (5‐azadC, 2 μM), G9a inhibitor (UNC0642, 2 μM) and pan‐HDAC inhibitor (ITF‐2357, 100 nM) for 48 h, or EZH2 inhibitor (EPZ‐6438, 10 μM) for 72h. KLF14 expression is measured by Western blotting analyses.

: Decitabine purchased from MedChemExpress. Usage Cited in: Oncogene. 2020 Aug;39(33):5507-5519. [Abstract]; Quantitative real-time PCR analysis of CDH13 expression after 5 μM Decitabine treatment.

: Decitabine purchased from MedChemExpress. Usage Cited in: J Invest Dermatol. 2020 Dec;140(12):2442-2454.e5. [Abstract]; In EZH2 mutant cells, after six days of drug treatment with both GSK126 and 0.5 μM Decitabine, Western blots shows complete elimination of H3K37me3 with reductions in DNMT1A, DNMT3A, and DNMT3B.

: Decitabine purchased from MedChemExpress. Usage Cited in: J Invest Dermatol. 2020 Dec;140(12):2442-2454.e5. [Abstract]; In EZH2 mutant cells, after six days of drug treatment with both GSK126 and 0.5 μM Decitabine, Significant upregulation of RASSF5, EPAS1, ITGB2, and NDRG1 target gene expression is seen in EZH2 mutant cell lines.

: Decitabine purchased from MedChemExpress. Usage Cited in: Ann Rheum Dis. 2019 Oct;78(10):1420-1429. [Abstract]; Western blot of PPARγ from mouse chondrocytes treated with IL-1β (10 ng/mL) for 24 hours in absence or presence 5Aza of various doses (0, 1, 5 and 10 µM).

: Decitabine purchased from MedChemExpress. Usage Cited in: Ann Rheum Dis. 2019 Oct;78(10):1420-1429. [Abstract]; Western blot of PPARγ, MMP13, Aamts5, Aggrecan and Collagen2 from Sham, DMM and 5Aza-treated mouse joint tissues (0.35 mg/kg; intraperitoneal injection daily; 10 weeks).

: Decitabine purchased from MedChemExpress. Usage Cited in: J Allergy Clin Immunol. 2019 Jun;143(6):2038-2051.e12. [Abstract]; Naive CD4⁺ T cells are stimulated with anti-CD3/CD28 in the presence of mock stimulant or Decitabine (5-aza-29-deoxycytidine; 1 µM). Real-time PCR analysis of Il4 and Il13 mRNA after 24 hours of stimulation and IL-4 and IL-13 concentrations in culture supernatants after 72 hours of stimulation are measured.

DNA Methyltransferase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

DNA Methyltransferase DNMT1 DNMT3 MGMT

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target^[5]

DNMT1

DNMT3A

DNMT3B

Cellular Effect

Cell Line	Type	Value	Description	References
LoVo	IC₅₀	21.9 μM Decitabine	Compound was tested for its inhibitory activity against LoVo cells. Compound was tested for its inhibitory activity against LoVo cells.	[PMID: 24874286]
SW111	IC₅₀	24.2 μM Decitabine	Compound was tested for its inhibitory activity against SW111 cells. Compound was tested for its inhibitory activity against SW111 cells.	[PMID: 24874286]

体外実験

Decitabine treatment significantly inhibits cell growth of SNU719, NCC24 and KATOIII 96 hours after exposure to decitabine. Decitabine induces G2/M arrest and apoptosis in EBVaGC, inhibits invasion ability, and up-regulates E-cadherin expression for EBVaGC^[1].
Only high concentrations (10 μM) Decitabine (0.1-1 μM; 24-72 hours) results in a G2 phase arrest, which is accompanied by a reduction of cells in G1 phase^[3].
Decitabine up-regulates DCTPP1 and dUTPase expression in HeLa cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:	HCT116 cells
Concentration:	0.1, 1, 10 µM
Incubation Time:	24, 48, 72 hours
Result:	Only high drug concentrations (10 µM) resulted in a G2 phase arrest, which was accompanied by a reduction of cells in G1 phase.

体内実験

Decitabine (1.0 mg/kg, p.o.) in combination with tetrahydrouridine (THU) causes severe toxicity occurs in female CD-1 mice, and results in an increased sensitivity to decitabine toxicity correlating with decitabine plasma levels^[5].
Decitabine (1.0 mg/kg; i.p.; once daily for 5 consecutive days) leads to regression of EL4 tumors established in C57BL/6 Mice^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice (bearing EL4 cells)^[6]
Dosage:	1.0 mg/kg
Administration:	Intraperitoneal injection; once daily for 5 consecutive days
Result:	Caused continuous tumor regression even after Decitabine treatment was stopped.

臨床実験

分子量

228.21

分子式

C₈H₁₂N₄O₄

CAS 番号

2353-33-5

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1C[C@H](N2C(N=C(N)N=C2)=O)O[C@@H]1CO

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶剤 & 溶解度

体外:

DMSO : ≥ 50 mg/mL (219.10 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 20 mg/mL (87.64 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.3819 mL	21.9096 mL	43.8193 mL
5 mM	0.8764 mL	4.3819 mL	8.7639 mL
10 mM	0.4382 mL	2.1910 mL	4.3819 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 10 mg/mL (43.82 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.97%

Select Batch:

データシート (281 KB) SDS (558 KB)

COA (252 KB) HNMR (199 KB) LCMS (347 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Nakamura M, et al. Decitabine inhibits tumor cell proliferation and up-regulates E-cadherin expression in Epstein-Barr virus-associated gastric cancer. J Med Virol. 2016 Jul 19. [Content Brief]

[2]. Parker WB. Enzymology of purine and pyrimidine antimetabolites used in the treatment of cancer. Chem Rev. 2009 Jul;109(7):2880-93. [Content Brief]

[3]. Hagemann S, et al. Azacytidine and decitabine induce gene-specific and non-random DNA demethylation in human cancer cell lines. PLoS One. 2011 Mar 7;6(3):e17388. [Content Brief]

[4]. Requena CE, et al. The nucleotidohydrolases DCTPP1 and dUTPase are involved in the cellular response to decitabine. Biochem J. 2016 Jun 20. [Content Brief]

[5]. Terse P, et al. Subchronic oral toxicity study of decitabine in combination with tetrahydrouridine in CD-1 mice. Int J Toxicol. 2014 Mar-Apr;33(2):75-85. [Content Brief]

[6]. Yu J, et al. DNA methyltransferase expression in triple-negative breast cancer predicts sensitivity to decitabine. J Clin Invest. 2018 Jun 1;128(6):2376-2388. [Content Brief]

[7]. Wang LX, et al. Low dose decitabine treatment induces CD80 expression in cancer cells and stimulates tumorspecific cytotoxic T lymphocyte responses. PLoS One. 2013 May 9;8(5):e62924. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.3819 mL	21.9096 mL	43.8193 mL	109.5482 mL
	5 mM	0.8764 mL	4.3819 mL	8.7639 mL	21.9096 mL
	10 mM	0.4382 mL	2.1910 mL	4.3819 mL	10.9548 mL
	15 mM	0.2921 mL	1.4606 mL	2.9213 mL	7.3032 mL
	20 mM	0.2191 mL	1.0955 mL	2.1910 mL	5.4774 mL
	25 mM	0.1753 mL	0.8764 mL	1.7528 mL	4.3819 mL
	30 mM	0.1461 mL	0.7303 mL	1.4606 mL	3.6516 mL
	40 mM	0.1095 mL	0.5477 mL	1.0955 mL	2.7387 mL
	50 mM	0.0876 mL	0.4382 mL	0.8764 mL	2.1910 mL
	60 mM	0.0730 mL	0.3652 mL	0.7303 mL	1.8258 mL
	80 mM	0.0548 mL	0.2739 mL	0.5477 mL	1.3694 mL
DMSO	100 mM	0.0438 mL	0.2191 mL	0.4382 mL	1.0955 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Decitabine Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Decitabine2353-33-55-Aza-2'-deoxycytidine 5-AZA-CdR NSC 127716NSC127716NSC 127716NSC-127716DNA MethyltransferaseApoptosisNucleoside Antimetabolite/AnalogDNMTsDNA MTasesInhibitorinhibitorinhibit

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デシタビン (Synonyms: Decitabine; 5-Aza-2′-Deoxycytidine; 5-AZA-CdR)

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Other Forms of Decitabine:

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DNA Methyltransferase アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Decitabine Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

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デシタビン (Synonyms: Decitabine; 5-Aza-2′-Deoxycytidine; 5-AZA-CdR)

カスタマーレビュー

Other Forms of Decitabine:

デシタビン 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

DNA Methyltransferase アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

デシタビン 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Decitabine Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report

デシタビン関連抗体

デシタビン関連抗体