Galanthamine (Synonyms: Galantamine)

Name: Galanthamine
Brand: MedChemExpress
SKU: HY-76299
Rating: 5.0 (15 reviews)

Cat. No.: HY-76299 Purity: 99.81%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Galanthamine is a potent acetylcholinesterase (AChE) inhibitor with an IC₅₀ of 500 nM.

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Galanthamine 화학구조

CAS No. : 357-70-0

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 15 publication(s) in Google Scholar

Other Forms of Galanthamine:

15 Publications Citing Use of MCE Galanthamine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Cholinesterase (ChE) Isoform Specific Products:

View All Isoforms

AChE BChE

Biological Activity
Protocol
순도&문서
References
고객리뷰

제품 설명

Galanthamine is a potent acetylcholinesterase (AChE) inhibitor with an IC₅₀ of 500 nM.

IC₅₀ & Target

AChE

Cellular Effect

Cell Line	Type	Value	Description	References
BJ	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against human BJ cells after 72 hrs by Calcein AM assay Cytotoxicity against human BJ cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
CCRF-CEM	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against human CEM cells after 72 hrs by Calcein AM assay Cytotoxicity against human CEM cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
Cortical neurone	EC₅₀	1.48 μM Compound: Galantamine	Neuroprotective activity against NMDA-induced excitotoxicity in Sprague-Dawley rat primary cortical neurons assessed as increase in cell viability after 3 hrs by MTT assay Neuroprotective activity against NMDA-induced excitotoxicity in Sprague-Dawley rat primary cortical neurons assessed as increase in cell viability after 3 hrs by MTT assay	[PMID: 27376495]
G-361	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against human G361 cells after 72 hrs by Calcein AM assay Cytotoxicity against human G361 cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
HeLa	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against human HeLa cells after 72 hrs by Calcein AM assay Cytotoxicity against human HeLa cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
HepG2	IC₅₀	4200 μM Compound: Galantamine	Cytotoxicity against human HepG2 cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay	[PMID: 36996715]
HepG2	IC₅₀	4200 μM Compound: Galantamine	Cytotoxicity against human HepG2 cells incubated for 24 hrs by MTT assay Cytotoxicity against human HepG2 cells incubated for 24 hrs by MTT assay	[PMID: 38784455]
K562	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against human K562 cells after 72 hrs by Calcein AM assay Cytotoxicity against human K562 cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
MCF7	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against human MCF7 cells after 72 hrs by Calcein AM assay Cytotoxicity against human MCF7 cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
NIH3T3	IC₅₀	7.28 μM Compound: Galanthamine	Cytotoxicity against mouse NIH/3T3 cells incubated for 24 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells incubated for 24 hrs by MTT assay	[PMID: 38614062]
SH-SY5Y	EC₅₀	1.94 μM Compound: Galantamine	Neuroprotective activity against okadaic acid-induced tau phosphorylation-mediated cell death in human SH-SY5Y cells assessed as increase in cell viability after 24 hrs by XTT assay Neuroprotective activity against okadaic acid-induced tau phosphorylation-mediated cell death in human SH-SY5Y cells assessed as increase in cell viability after 24 hrs by XTT assay	[PMID: 27376495]
SH-SY5Y	EC₅₀	2.81 μM Compound: Galantamine	Neuroprotective activity against oligomycine/rotenone-induced oxidative stress-mediated cell death in human SH-SY5Y cells assessed as increase in cell viability after 24 hrs by XTT assay Neuroprotective activity against oligomycine/rotenone-induced oxidative stress-mediated cell death in human SH-SY5Y cells assessed as increase in cell viability after 24 hrs by XTT assay	[PMID: 27376495]
SH-SY5Y	IC₅₀	4700 μM Compound: Galantamine	Cytotoxicity against human SH-SY5Y cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay Cytotoxicity against human SH-SY5Y cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay	[PMID: 36996715]
SH-SY5Y	IC₅₀	4700 μM Compound: Galantamine	Cytotoxicity against human SH-SY5Y cells incubated for 24 hrs by MTT assay Cytotoxicity against human SH-SY5Y cells incubated for 24 hrs by MTT assay	[PMID: 38784455]
SH-SY5Y	IC₅₀	747 nM Compound: 1	Neuroprotective activity in human SH-SY5Y cells assessed as protection against NMDA-induced cell death after 6 hrs by MTS assay Neuroprotective activity in human SH-SY5Y cells assessed as protection against NMDA-induced cell death after 6 hrs by MTS assay	[PMID: 23033965]

In Vitro

Galanthamine inhibits AChE and BChE with IC₅₀ of 0.5 and 8.5 μM^[1]. Galanthamine acts as a positive allosteric modulator (PAM) of human α4β2 AChRs expressed in permanently transfected HEK 293 cells. Galanthamine increases the response of (α4β2)₂α5 AChRs to 1 μM ACh by up to 220% with very low concerntration(EC₅₀=0.25 nM). Only small potentiation (20%) of either α4β2 or (α4β2)₂β3 AChRs is detected using FLEXstation assays. Galanthamine at concentrations of 1 μM and above inhibits all three AChR subtypes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Acute administration of Galantamine (0.3-3 mg/kg, i.p.) increases IGF2 mRNA levels in the hippocampus, but not in the prefrontal cortex, in time- and dose-dependent manner. Galantamine (3 mg/kg, i.p.) causes a transient increase in fibroblast growth factor 2 mRNA levels and a decrease in brain-derived neurotrophic factor mRNA levels in the hippocampus, while it does not affect the mRNA levels of other neurotrophic/growth factors. The Galantamine-induced increase in the hippocampal IGF2 mRNA levels is blocked by Mecamylamine, a nonselective nicotinic acetylcholine (ACh) receptor (nAChR) antagonist, and Methyllycaconitine, a selective α7 nAChR antagonist, but not by Telenzepine, a preferential M1muscarinic ACh receptor antagonist. Moreover, the selective α7 nAChR agonist PHA-543613 increasea the IGF2 mRNA levels, while Donepezil, an acetylcholinesterase inhibitor, does not. Galantamine also increases hippocampal IGF2 protein, which is blocked by Methyllycaconitine^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

분자량

287.36

화학식

C₁₇H₂₁NO₃

CAS No.

357-70-0

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1C[C@@H]2OC3=C4C(CN(C)CC[C@]42C=C1)=CC=C3OC

Structure Classification

Initial Source

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

용액&용해도

In Vitro:

DMSO : ≥ 59 mg/mL (205.32 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4800 mL	17.3998 mL	34.7997 mL
5 mM	0.6960 mL	3.4800 mL	6.9599 mL
10 mM	0.3480 mL	1.7400 mL	3.4800 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.90%

Select Batch:

Data Sheet (282 KB) SDS (481 KB)

COA (254 KB) HNMR (195 KB) RP-HPLC (266 KB) LCMS (293 KB)

Handling Instructions (2659 KB)

References

[1]. Melanie-Jayne R. Howes, et al. Acetylcholinesterase inhibitors of natural origin. International Journal of Research in Pharmaceutical and Biomedical Sciences 3(SI 1):67-86.

[2]. Kuryatov A, et al. Roles of accessory subunits in alpha4beta2(*) nicotinic receptors. Mol Pharmacol. 2008 Jul;74(1):132-43. [Content Brief]

[3]. Kita Y, et al. Galantamine increases hippocampal insulin-like growth factor 2 expression via α7 nicotinic acetylcholine receptors in mice. Psychopharmacology (Berl). 2013 Feb;225(3):543-51. [Content Brief]

Animal Administration
^[2]

Mice^[2]
Eight-week-old male ddY mice are housed in cages (24 cm×17 cm×12 cm) in each group of five to six animals under controlled environmental conditions (22±1°C; 12:12-h light-dark cycle, lights on at 0800 hours, food and water ad libitum) for 1 week before use in the experiments. 453 mice are used in total and in single use for each purpose. The following drugs are used: mecamylamine, methyllycaconitine, oxotremorine, and telenzepine, and Galantamine, Donepezil, and PHA-543613. All drugs are dissolved in saline (0.9 % solution of NaCl). Drugs are administered in a volume of 10 mL/kg intraperitoneally (i.p.) (Galantamine, Donepezil, Mecamylamine, Methyllycaconitine, Oxotremorine) or subcutaneously (s.c.) (PHA-543613, Telenzepine).

MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.

References

[1]. Melanie-Jayne R. Howes, et al. Acetylcholinesterase inhibitors of natural origin. International Journal of Research in Pharmaceutical and Biomedical Sciences 3(SI 1):67-86.

[2]. Kuryatov A, et al. Roles of accessory subunits in alpha4beta2(*) nicotinic receptors. Mol Pharmacol. 2008 Jul;74(1):132-43. [Content Brief]

[3]. Kita Y, et al. Galantamine increases hippocampal insulin-like growth factor 2 expression via α7 nicotinic acetylcholine receptors in mice. Psychopharmacology (Berl). 2013 Feb;225(3):543-51. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4800 mL	17.3998 mL	34.7997 mL	86.9992 mL
	5 mM	0.6960 mL	3.4800 mL	6.9599 mL	17.3998 mL
	10 mM	0.3480 mL	1.7400 mL	3.4800 mL	8.6999 mL
	15 mM	0.2320 mL	1.1600 mL	2.3200 mL	5.7999 mL
	20 mM	0.1740 mL	0.8700 mL	1.7400 mL	4.3500 mL
	25 mM	0.1392 mL	0.6960 mL	1.3920 mL	3.4800 mL
	30 mM	0.1160 mL	0.5800 mL	1.1600 mL	2.9000 mL
	40 mM	0.0870 mL	0.4350 mL	0.8700 mL	2.1750 mL
	50 mM	0.0696 mL	0.3480 mL	0.6960 mL	1.7400 mL
	60 mM	0.0580 mL	0.2900 mL	0.5800 mL	1.4500 mL
	80 mM	0.0435 mL	0.2175 mL	0.4350 mL	1.0875 mL
	100 mM	0.0348 mL	0.1740 mL	0.3480 mL	0.8700 mL

Galanthamine Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Galanthamine357-70-0GalantamineCholinesterase (ChE)ApoptosisInhibitorinhibitorinhibit

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Galanthamine (Synonyms: Galantamine)

고객리뷰

Other Forms of Galanthamine:

Galanthamine Related Antibodies

15 Publications Citing Use of MCE Galanthamine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Cholinesterase (ChE) Isoform Specific Products:

Biological Activity

Protocol

순도&문서

References

고객리뷰

Galanthamine Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Galanthamine Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report