Minoxidil (Synonyms: U10858)

Name: Minoxidil
Brand: MedChemExpress
SKU: HY-B0112
Rating: 5.0 (11 reviews)

Cat. No.: HY-B0112 Purity: 99.99%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Minoxidil (U10858) is an ATP-sensitive potassium (K_ATP) channel opener, a potent oral antihypertensive agent and a peripheral vasodilator that promotes vasodilation also affects hair growth. Minoxidil is also a potent inhibitor of soybean lipoxygenaseare with an IC₅₀ of 20 μM.

For research use only. We do not sell to patients.

CAS No. : 38304-91-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

or Bulk Inquiry

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of Minoxidil:

11 Publications Citing Use of MCE Minoxidil

Cell Imaging/Staining

Histological Imaging/Staining

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

IHC

: Minoxidil purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2025 Nov 14:57:344-357. [Abstract]; Cell proliferation assay in DPCs and HFSCs treated with DHT (500 μM) and DHT combined with Minoxidil (20 μM), tFNFs (0.25 μM), Que (20 μM), and TQC (tFNFs/Que: 0.25/20 μM) for 24 h.

: Minoxidil purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2025 Nov 14:57:344-357. [Abstract]; Time-course observations of hair growth of samples at 1, 4, 7, 10, 14, 15, 18, 21, 24, and 28 days (n = 5). The results showed that Minoxidil (5%; topical application, 100 μL per application) treatment, serving as a positive control, led to gradual hair regrowth starting from day 10, with marked hair coverage observed by day 28.

: Minoxidil purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Oct 16:S2090-1232(25)00818-5. [Abstract]; Minoxidil (0.5 mM； 48 h) led to a significant increase in the expression of epithelial differentiation markers in 786-O sccRCC cells.

: Minoxidil purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Oct 16:S2090-1232(25)00818-5. [Abstract]; Minoxidil (0.25-0.5 mM; 48 h) did not cause substantial 786-O ccRCC cell death.

: Minoxidil purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Oct 16:S2090-1232(25)00818-5. [Abstract]; Minoxidil (6 mg/kg; gavage) significantly reduced the Ki-67 index in sccRCC xenograft mouse models.

: Minoxidil purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Oct 16:S2090-1232(25)00818-5. [Abstract]; Minoxidil (6 mg/kg; gavage) significantly reduced the tumor growth rate in sccRCC xenograft mouse models.

: Minoxidil purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2024 Jan 4;31(1):52-70.e8. [Abstract]; Representative bright-field images of ARPKD (ARPKD Epi-17) kidney organoids treated with a concentration gradient of ST034307 and Minoxidil (0.1-5 μM) in the presence of FSK for 3 days. Scale bars, 200 μm.

: Minoxidil purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2024 Jan 4;31(1):52-70.e8. [Abstract]; Minoxidil (i.p.; once daily for 3 weeks) administered at 1 and 5 mg/kg/day reduced the calculated cystic index of ARPKD kidney organoid xenografts from host NSG mice by 18.85% and 33.93%, respectively.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 20 μM (soybean lipoxygenaseare)^[1]; ATP-sensitive potassium channel^[2]

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	> 10 μM Compound: MNX	Induction of cell differentiation in human HL60 cells by NBT/MTT assay Induction of cell differentiation in human HL60 cells by NBT/MTT assay	[PMID: 26832215]

In Vitro

Minoxidil (1-00 μM; 24 hours; RAMEC cells) treatment shows very low cytotoxicities in the whole area of concentrations examined (from 1 μM to 100 μM)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Minoxidil (0.01 mmoL/kg body weight; intraperitoneal injection; for 3.5 hours; fisher 344 rats) treatment inhibits carrageenan-induced rat paw oedema with an inhibitory potency (49%)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Fisher 344 rats (150-200 g) with oedema^[1]
Dosage:	0.01 mmol/kg body weight
Administration:	Intraperitoneal injection; for 3.5 hours
Result:	Inhibition of the carrageenin-induced oedema.

Molecular Weight

209.25

Formula

C₉H₁₅N₅O

CAS No.

38304-91-5

Appearance

Solid

Color

White to off-white

SMILES

NC1=NC(N2CCCCC2)=CC(N)=[N+]1[O-]

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

Ethanol : 7.14 mg/mL (34.12 mM; Need ultrasonic)

DMSO : 5 mg/mL (23.89 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (4.78 mM; ultrasonic and warming and heat to 50°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.7790 mL	23.8949 mL	47.7897 mL
5 mM	0.9558 mL	4.7790 mL	9.5579 mL
10 mM	0.4779 mL	2.3895 mL	4.7790 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.71 mg/mL (3.39 mM); Clear solution

This protocol yields a clear solution of ≥ 0.71 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (7.1 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.71 mg/mL (3.39 mM); Clear solution

This protocol yields a clear solution of ≥ 0.71 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (7.1 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 0.71 mg/mL (3.39 mM); Clear solution

This protocol yields a clear solution of ≥ 0.71 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (7.1 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.5 mg/mL (2.39 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 5

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.5 mg/mL (2.39 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 6

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.5 mg/mL (2.39 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 1.96 mg/mL (9.37 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Protocol 2

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 5 mg/mL (23.89 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (253 KB) HNMR (479 KB) RP-HPLC (233 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Hadjipavlou-Litina D, et al. Synthesis and evaluation of the antioxidative potential of minoxidil-polyamine conjugates. Biochimie. 2013 Jul;95(7):1437-49. doi: 10.1016/j.biochi.2013.03.009. Epub 2013 Mar 28. [Content Brief]

[2]. Davies GC, et al. Novel and established potassium channel openers stimulate hair growth in vitro: implications for their modes of action in hair follicles. J Invest Dermatol. 2005 Apr;124(4):686-94. [Content Brief]

[3]. Cohen RL, et al. Direct effects of minoxidil on epidermal cells in culture. J Invest Dermatol. 1984 Jan;82(1):90-3. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO / Ethanol	1 mM	4.7790 mL	23.8949 mL	47.7897 mL	119.4743 mL
DMSO / Ethanol	5 mM	0.9558 mL	4.7790 mL	9.5579 mL	23.8949 mL
	10 mM	0.4779 mL	2.3895 mL	4.7790 mL	11.9474 mL
	15 mM	0.3186 mL	1.5930 mL	3.1860 mL	7.9650 mL
	20 mM	0.2389 mL	1.1947 mL	2.3895 mL	5.9737 mL
Ethanol	25 mM	0.1912 mL	0.9558 mL	1.9116 mL	4.7790 mL
Ethanol	30 mM	0.1593 mL	0.7965 mL	1.5930 mL	3.9825 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Minoxidil Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Minoxidil38304-91-5U10858U 10858U-10858Potassium ChannelEndogenous MetaboliteKcsAInhibitorinhibitorinhibit

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Minoxidil (Synonyms: U10858)

Customer Review

Other Forms of Minoxidil:

Minoxidil Related Antibodies

11 Publications Citing Use of MCE Minoxidil

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Minoxidil Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Minoxidil Related Classifications

Keywords:

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Customer Review

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