S-Adenosyl-L-methionine disulfate tosylate
Based on 22 publication(s) in Google Scholar
S-Adenosyl-L-methionine (Ademetionine) disulfate tosylate is the disulfate tosylate form of S-Adenosyl-L-methionine (HY-B0617). S-Adenosyl-L-methionine disulfate tosylate is an orally active methyl group donor. S-Adenosyl-L-methionine disulfate tosylate is a dietary supplement with potent antidepressant effects. S-Adenosyl-L-methionine disulfate tosylate also has anti‑proliferative, pro‑apoptotic and anti‑metastatic roles in cancers. S-Adenosyl-L-methionine disulfate tosylate has the potential for, cancer, liver disease and osteoarthritis research.
For research use only. We do not sell to patients.
- Purity : 99.50%
- CAS No.: 97540-22-2
- Formula: C22H34N6O16S4
- Molecular Weight:766.80
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) S-Adenosyl-L-methionine disulfate tosylate
More- Mol Cancer. 2025 Nov 20;24(1):295. [Abstract]
- Autophagy. 2026 May 17:1-23. [Abstract]
- Nat Commun. 2024 Nov 12;15(1):9529. [Abstract]
- Gut Microbes. 2025 Dec;17(1):2545434. [Abstract]
- MedComm (2020). 2026 Jul 5;7(7):e70831.
- Environ Sci Technol. 2025 Jan 14;59(1):268-278. [Abstract]
- Pharmacol Res. 2024 Nov:209:107420. [Abstract]
- J Exp Med. 2026 Jan 5;223(1):e20250603. [Abstract]
- Int J Biol Macromol. 2026 Apr:355:151129. [Abstract]
- J Agric Food Chem. 2021 Aug 11;69(31):8737-8746. [Abstract]
- Biochem Pharmacol. 2024 Jan:219:115967. [Abstract]
- Ind Crops Prod. 2025 Dec 2;238:122414.
- Food Funct. 2025 Oct 27. [Abstract]
- Plant J. 2025 Apr;122(2):e70182. [Abstract]
- Int Immunopharmacol. 2021 Jun:95:107545. [Abstract]
- Epigenetics Chromatin. 2021 Dec 4;14(1):52. [Abstract]
- SSRN. 2026 Jun 23.
- Biomed Pharmacother. 2025 Aug:189:118246. [Abstract]
- bioRxiv. 2025 Jun 27.
- bioRxiv. 2023 Jun 1.
- Research Square Preprint. 2021 Jun.
- Research Square Preprint. 2020 Aug.
Customer Validation & Images
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Flow Cytometry
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Cell Proliferation/Viability Assay
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WB
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WB
All Endogenous Metabolite Isoforms
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Biological Activity
Description
IC50 & Target
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Human Endogenous Metabolite |
In Vitro
S-Adenosyl-L-methionine (300 µM, 24 or 48 h) disulfate tosylate induces cell apoptosis, and promotes the cell cycle arrest in Cal-33 and JHU-SCC-011 cells[4].
S-Adenosyl-L-methionine (300 µM, 24 h) disulfate tosylate decreases the migration of the Cal-33 and JHU-SCC-011 cells[4].
S-Adenosyl-L-methionine (5-40 μg/mL, 48 h) disulfate tosylate protects the anticancer effect of 5‑FU by regulating the expression of DNMTs[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Cal-33 and JHU-SCC-011 cells
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Concentration:300 µM
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Incubation Time:24 h (Cal-33) or 48 h (HU-SCC-011)
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Result:Showed an approximately 10% and 3%of apoptotic cells respectively.
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Cell Line:Cal-33 and JHU-SCC-011 cells
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Concentration:300 µM
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Incubation Time:24 h (Cal-33) or 48 h (HU-SCC-011)
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Result:Decreased the expression of cyclin B1, E1 and D1 in the Cal-33 and JHU-SCC-011 cells.
In Vivo
S-Adenosyl-L-methionine (50 and 100 mg/kg, p.o.) disulfate tosylate shows antiepileptic, memory-enhancing, and antioxidant properties in a Pentylenetetrazole-induced rat epilepsy model[7].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Valproic acid treated young mice[6]
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Dosage:30 mg/kg
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Administration:p.o., for 3 days
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Result:Alleviated most ASD like neurobehavioral symptoms.
Normalized the redox potential in the prefrontal cortex.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 97540-22-2
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Appearance Solid
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Molecular Weight 766.80
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Formula C22H34N6O16S4
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Color White to light yellow
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SMILES
N[C@@H](CC[S+](C[C@@H]1[C@H]([C@H]([C@H](N2C=NC3=C2N=CN=C3N)O1)O)O)C)C(O)=O.O=S(O)([O-])=O.O=S(O)(O)=O.OS(=O)(C4=CC=C(C)C=C4)=O
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Synonyms
Ademetionine disulfate tosylate; S-Adenosyl methionine disulfate tosylate; AdoMet disulfate tosylate
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (22)
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Journal Impact Factor
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Most Recent
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Mol Cancer
ZNF526 drives tumor growth by enhancing SHMT1-dependent serine metabolism and antioxidant capability in TNBC. [Abstract]2025 Nov 20;24(1):295. PMID: 41267037 -
Autophagy
SETDB1 enhances starvation-induced lipophagy by inhibiting m6A-mediated mRNA decay via DDX5 methylation. [Abstract]2026 May 17:1-23. PMID: 42087801 -
Nat Commun
2024 Nov 12;15(1):9529. PMID: 39532843 -
Gut Microbes
Gut microbiota-mediated betaine regulates skeletal muscle fiber type transition by affecting m6A RNA methylation and Myh7 expression. [Abstract]2025 Dec;17(1):2545434. PMID: 40824213 -
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Environ Sci Technol
Arsenic Exposure Triggers Nonalcoholic Fatty Liver Disease through Repressing S-Adenosylmethionine-Dependent Histone Methylation in Rats. [Abstract]2025 Jan 14;59(1):268-278. PMID: 39746780 -
Pharmacol Res
Developing patient-derived organoids to demonstrate JX24120 inhibits SAMe synthesis in endometrial cancer by targeting MAT2B. [Abstract]2024 Nov:209:107420. PMID: 39293586
S-Adenosyl-L-methionine disulfate tosylate purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Nov:209:107420. [Abstract]
Cell viability was determined in response to JX24120 with the supplement of exogenous S-Adenosyl-L-methionine disulfate tosylate (SAMe, 5-10 μM). The results demonstrated that SAMe effectively reversed the cytotoxicity induced by JX24120 in endometrial cancer organoids and cell lines.
S-Adenosyl-L-methionine disulfate tosylate purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Nov:209:107420. [Abstract]
The protein synthesis rates of treated organoids, ISK and KLE were examined by puromycin incorporation assay. In organoids and cell lines, JX24120 inhibited protein synthesis in a dose-dependent manner, which was also rescued by exogenous supplementation with S-Adenosyl-L-methionine disulfate tosylate (SAMe, 10 μM).
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J Exp Med
Adenosine metabolic clearance maintains liver homeostasis by licensing arginine methylation of RIPK1. [Abstract]2026 Jan 5;223(1):e20250603. PMID: 41081715 -
Int J Biol Macromol
Targeting OXCT1 with the methyl donor S-adenosylmethionine as a therapeutic strategy for cerebral cavernous malformations. [Abstract]2026 Apr:355:151129. PMID: 41865434 -
J Agric Food Chem
Sesamol Epigenetically Induces Estrogen Receptor α Re-expression by Upregulating miR-370-3p in Estrogen Receptor α-Negative Breast Cancer. [Abstract]2021 Aug 11;69(31):8737-8746. PMID: 34325508 -
Biochem Pharmacol
S-adenosylmethionine attenuates angiotensin II-induced aortic dissection formation by inhibiting vascular smooth muscle cell phenotypic switch and autophagy. [Abstract]2024 Jan:219:115967. PMID: 38065291 -
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Food Funct
Methionine restriction attenuates renal injury by suppressing macrophage polarization via IRF1 downregulation. [Abstract]2025 Oct 27. PMID: 41140247
S-Adenosyl-L-methionine disulfate tosylate purchased from MedChemExpress. Usage Cited in: Food Funct. 2025 Oct 27. [Abstract]
Representative flow cytometry plots of CD86+ and CD206+ BMDMs cultured under different treatment conditions (n = 5). The results showed that S-Adenosyl-L-methionine disulfate tosylate (SAM, 100 μM) supplementation reversed the aberrant polarization phenotype of BMDMs.
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Plant J
Transcriptomic and metabolomic analyses unveil TaASMT3-mediated wheat resistance against stripe rust by promoting melatonin biosynthesis. [Abstract]2025 Apr;122(2):e70182. PMID: 40298361 -
Int Immunopharmacol
Hydrogen sulfide ameliorates high glucose-induced pro-inflammation factors in HT-22 cells: Involvement of SIRT1-mTOR/NF-κB signaling pathway. [Abstract]2021 Jun:95:107545. PMID: 33765609
S-Adenosyl-L-methionine disulfate tosylate purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2021 Jun:95:107545. [Abstract]
S-adenosylmethionine (SAMe) application at 30 min prior to high glucose treatment significantly increased H2S levels compared with the HG group. The cystathionine-β-synthase (CBS) level is obviously elevated in HG + SAMe group compared with the HG group.
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Epigenetics Chromatin
Ethionine-mediated reduction of S-adenosylmethionine is responsible for the neural tube defects in the developing mouse embryo-mediated m6A modification and is involved in neural tube defects via modulating Wnt/β-catenin signaling pathway. [Abstract]2021 Dec 4;14(1):52. PMID: 34863249 -
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Biomed Pharmacother
Identification of nsp16 inhibitors of SARS -CoV-2, SARS -CoV-1 and MERS-CoV from FDA-approved drugs using in silico and in vitro methods. [Abstract]2025 Aug:189:118246. PMID: 40543162 -
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Solvent & Solubility
In Vitro:
H2O : 250 mg/mL (326.03 mM; Need ultrasonic)
DMSO : ≥ 50 mg/mL (65.21 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.26 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.26 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (130.41 mM); Clear solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Bressa GM. S-adenosyl-l-methionine (SAMe) as antidepressant: meta-analysis of clinical studies. Acta Neurol Scand Suppl. 1994;154:7-14. [Content Brief]
[2]. Najm WI, et al. S-adenosyl methionine (SAMe) versus celecoxib for the treatment of osteoarthritis symptoms: a double-blind cross-over trial. [ISRCTN36233495]. BMC Musculoskelet Disord. 2004 Feb 26;5:6. [Content Brief]
[3]. Lu SC, et al. S-adenosylmethionine in liver health, injury, and cancer. Physiol Rev. 2012 Oct;92(4):1515-42. [Content Brief]
[4]. Mosca L, et al. Effects of S‑adenosyl‑L‑methionine on the invasion and migration of head and neck squamous cancer cells and analysis of the underlying mechanisms. Int J Oncol. 2020 May;56(5):1212-1224. [Content Brief]
[5]. Ham MS, et al. S-adenosyl methionine specifically protects the anticancer effect of 5-FU via DNMTs expression in human A549 lung cancer cells. Mol Clin Oncol. 2013 Mar;1(2):373-378. [Content Brief]
[6]. Ornoy A, et al. S-adenosyl methionine prevents ASD like behaviors triggered by early postnatal valproic acid exposure in very young mice. Neurotoxicol Teratol. 2019 Jan-Feb;71:64-74. [Content Brief]
[7]. Dhediya RM, et al. Evaluation of antiepileptic effect of S-adenosyl methionine and its role in memory impairment in pentylenetetrazole-induced kindling model in rats. Epilepsy Behav. 2016 Aug;61:153-157. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.3041 mL | 6.5206 mL | 13.0412 mL | 32.6030 mL |
| 5 mM | 0.2608 mL | 1.3041 mL | 2.6082 mL | 6.5206 mL | |
| 10 mM | 0.1304 mL | 0.6521 mL | 1.3041 mL | 3.2603 mL | |
| 15 mM | 0.0869 mL | 0.4347 mL | 0.8694 mL | 2.1735 mL | |
| 20 mM | 0.0652 mL | 0.3260 mL | 0.6521 mL | 1.6302 mL | |
| 25 mM | 0.0522 mL | 0.2608 mL | 0.5216 mL | 1.3041 mL | |
| 30 mM | 0.0435 mL | 0.2174 mL | 0.4347 mL | 1.0868 mL | |
| 40 mM | 0.0326 mL | 0.1630 mL | 0.3260 mL | 0.8151 mL | |
| 50 mM | 0.0261 mL | 0.1304 mL | 0.2608 mL | 0.6521 mL | |
| 60 mM | 0.0217 mL | 0.1087 mL | 0.2174 mL | 0.5434 mL | |
| H2O | 80 mM | 0.0163 mL | 0.0815 mL | 0.1630 mL | 0.4075 mL |
| 100 mM | 0.0130 mL | 0.0652 mL | 0.1304 mL | 0.3260 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
S-Adenosyl-L-methionine disulfate tosylate Related Classifications
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