Cat. No.: HY-B0617A

S-Adenosyl-L-methionine tosylate

Name: S-Adenosyl-L-methionine tosylate
Brand: MedChemExpress
SKU: HY-B0617A
Price: 84.00 USD
Availability: InStock
Rating: 5 (22 reviews)

(Synonyms: S-Adenosyl methionine tosylate; Ademetionine tosylate; AdoMet tosylate)

22 Cited Publications

Customer Review

Based on 22 publication(s) in Google Scholar

S-Adenosyl-L-methionine (S-Adenosyl methionine) tosylate is an orally active methyl group donor. S-Adenosyl-L-methionine tosylate is a dietary supplement with potent antidepressant effects. S-Adenosyl-L-methionine tosylate also has anti‑proliferative, pro‑apoptotic and anti‑metastatic roles in cancers. S-Adenosyl-L-methionine tosylate has the potential for, cancer, liver disease and osteoarthritis research.

For research use only. We do not sell to patients.

Purity: 99.59%
CAS No.: 52248-03-0
Formula: C₂₂H₃₀N₆O₈S₂
Molecular Weight:570.64

Storage:

-20°C, stored under nitrogen, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

S-Adenosyl-L-methionine tosylate Chemical Structure

CAS No.: 52248-03-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (22)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

S-Adenosyl-L-methionine (300 µM, 24 or 48 h) tosylate induces cell apoptosis, and promotes the cell cycle arrest in Cal-33 and JHU-SCC-011 cells^[4].
S-Adenosyl-L-methionine (300 µM, 24 h) tosylate decreases the migration of the Cal-33 and JHU-SCC-011 cells^[4].
S-Adenosyl-L-methionine (5-40 μg/mL, 48 h) tosylate protects the anticancer effect of 5‑FU by regulating the expression of DNMTs^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[4]

Cell Line:

Cal-33 and JHU-SCC-011 cells
Concentration:

300 µM
Incubation Time:

24 h (Cal-33) or 48 h (HU-SCC-011)
Result:

Showed an approximately 10% and 3%of apoptotic cells respectively.

Western Blot Analysis^[4]

Cell Line:

Cal-33 and JHU-SCC-011 cells
Concentration:

300 µM
Incubation Time:

24 h (Cal-33) or 48 h (HU-SCC-011)
Result:

Decreased the expression of cyclin B1, E1 and D1 in the Cal-33 and JHU-SCC-011 cells.

In Vivo

S-Adenosyl-L-methionine (30 mg/kg, p.o., for 3 days) tosylate prevents ASD like behaviors induced by early postnatal valproic acid exposure in young mice^[6].
S-Adenosyl-L-methionine (50 and 100 mg/kg, p.o.) tosylate shows antiepileptic, memory-enhancing, and antioxidant properties in a Pentylenetetrazole-induced rat epilepsy model^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Valproic acid treated young mice^[6]
Dosage:

30 mg/kg
Administration:

p.o., for 3 days
Result:

Alleviated most ASD like neurobehavioral symptoms.
Normalized the redox potential in the prefrontal cortex.

Chemical Information

CAS No. 52248-03-0
Appearance Solid
Molecular Weight 570.64
Formula C₂₂H₃₀N₆O₈S₂
Color White to yellow
SMILES

O[C@@H]([C@H]([C@H](N1C=NC2=C(N=CN=C21)N)O3)O)[C@H]3C[S+](CC[C@H](N)C(O)=O)C.O=S(C4=CC=C(C)C=C4)([O-])=O
Synonyms

S-Adenosyl methionine tosylate; Ademetionine tosylate; AdoMet tosylate
Structure Classification
- Others
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, stored under nitrogen, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Publications (22)

All Cell Proliferation/Viability AssayFlow CytometryWB

Sort by:

Journal Impact Factor

Mol Cancer

ZNF526 drives tumor growth by enhancing SHMT1-dependent serine metabolism and antioxidant capability in TNBC. [Abstract]

2025 Nov 20;24(1):295. PMID: 41267037
Autophagy

SETDB1 enhances starvation-induced lipophagy by inhibiting m6A-mediated mRNA decay via DDX5 methylation. [Abstract]

2026 May 17:1-23. PMID: 42087801
Nat Commun

Nuclear localization of MTHFD2 is required for correct mitosis progression. [Abstract]

2024 Nov 12;15(1):9529. PMID: 39532843
Gut Microbes

Gut microbiota-mediated betaine regulates skeletal muscle fiber type transition by affecting m6A RNA methylation and Myh7 expression. [Abstract]

2025 Dec;17(1):2545434. PMID: 40824213
Environ Sci Technol

Arsenic Exposure Triggers Nonalcoholic Fatty Liver Disease through Repressing S-Adenosylmethionine-Dependent Histone Methylation in Rats. [Abstract]

2025 Jan 14;59(1):268-278. PMID: 39746780
Pharmacol Res

Developing patient-derived organoids to demonstrate JX24120 inhibits SAMe synthesis in endometrial cancer by targeting MAT2B. [Abstract]

2024 Nov:209:107420. PMID: 39293586

S-Adenosyl-L-methionine tosylate purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Nov:209:107420. [Abstract]

Cell viability was determined in response to JX24120 with the supplement of exogenous S-Adenosyl-L-methionine disulfate tosylate (SAMe, 5-10 μM). The results demonstrated that SAMe effectively reversed the cytotoxicity induced by JX24120 in endometrial cancer organoids and cell lines.

S-Adenosyl-L-methionine tosylate purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Nov:209:107420. [Abstract]

The protein synthesis rates of treated organoids, ISK and KLE were examined by puromycin incorporation assay. In organoids and cell lines, JX24120 inhibited protein synthesis in a dose-dependent manner, which was also rescued by exogenous supplementation with S-Adenosyl-L-methionine disulfate tosylate (SAMe, 10 μM).
J Exp Med

Adenosine metabolic clearance maintains liver homeostasis by licensing arginine methylation of RIPK1. [Abstract]

2026 Jan 5;223(1):e20250603. PMID: 41081715
Int J Biol Macromol

Targeting OXCT1 with the methyl donor S-adenosylmethionine as a therapeutic strategy for cerebral cavernous malformations. [Abstract]

2026 Apr:355:151129. PMID: 41865434
J Agric Food Chem

Sesamol Epigenetically Induces Estrogen Receptor α Re-expression by Upregulating miR-370-3p in Estrogen Receptor α-Negative Breast Cancer. [Abstract]

2021 Aug 11;69(31):8737-8746. PMID: 34325508
Biochem Pharmacol

S-adenosylmethionine attenuates angiotensin II-induced aortic dissection formation by inhibiting vascular smooth muscle cell phenotypic switch and autophagy. [Abstract]

2024 Jan:219:115967. PMID: 38065291
Ind Crops Prod

2025 Dec 2;238:122414.
Food Funct

Methionine restriction attenuates renal injury by suppressing macrophage polarization via IRF1 downregulation. [Abstract]

2025 Oct 27. PMID: 41140247

S-Adenosyl-L-methionine tosylate purchased from MedChemExpress. Usage Cited in: Food Funct. 2025 Oct 27. [Abstract]

Representative flow cytometry plots of CD86⁺ and CD206⁺ BMDMs cultured under different treatment conditions (n = 5). The results showed that S-Adenosyl-L-methionine disulfate tosylate (SAM, 100 μM) supplementation reversed the aberrant polarization phenotype of BMDMs.
Plant J

Transcriptomic and metabolomic analyses unveil TaASMT3-mediated wheat resistance against stripe rust by promoting melatonin biosynthesis. [Abstract]

2025 Apr;122(2):e70182. PMID: 40298361
Int Immunopharmacol

Hydrogen sulfide ameliorates high glucose-induced pro-inflammation factors in HT-22 cells: Involvement of SIRT1-mTOR/NF-κB signaling pathway. [Abstract]

2021 Jun:95:107545. PMID: 33765609

S-Adenosyl-L-methionine tosylate purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2021 Jun:95:107545. [Abstract]

S-adenosylmethionine (SAMe) application at 30 min prior to high glucose treatment significantly increased H2S levels compared with the HG group. The cystathionine-β-synthase (CBS) level is obviously elevated in HG + SAMe group compared with the HG group.
Epigenetics Chromatin

Ethionine-mediated reduction of S-adenosylmethionine is responsible for the neural tube defects in the developing mouse embryo-mediated m6A modification and is involved in neural tube defects via modulating Wnt/β-catenin signaling pathway. [Abstract]

2021 Dec 4;14(1):52. PMID: 34863249
J Pharm Biomed Anal

Quantitative determination of ademetionine in tablets utilizing ATR-FTIR and partial least squares methods approaches. [Abstract]

2024 Apr 15:241:115991. PMID: 38301577
SSRN

2026 Jun 23.
Biomed Pharmacother

Identification of nsp16 inhibitors of SARS -CoV-2, SARS -CoV-1 and MERS-CoV from FDA-approved drugs using in silico and in vitro methods. [Abstract]

2025 Aug:189:118246. PMID: 40543162
bioRxiv

2025 Jun 27.
bioRxiv

2023 Jun 1.
Research Square Preprint

2021 Jun.
Research Square Preprint

2020 Aug.

Solvent & Solubility

In Vitro:

H₂O : 175 mg/mL (306.67 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7524 mL

8.7621 mL

17.5242 mL

5 mM

0.3505 mL

1.7524 mL

3.5048 mL

10 mM

0.1752 mL

0.8762 mL

1.7524 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. G M Bressa. et al. S-adenosyl-l-methionine (SAMe) as antidepressant: meta-analysis of clinical studies. Acta Neurol Scand Suppl. 1994;154:7-14. [Content Brief]

[2]. Wadie I Najm, et al. S-adenosyl methionine (SAMe) versus celecoxib for the treatment of osteoarthritis symptoms: a double-blind cross-over trial. [ISRCTN36233495]. BMC Musculoskelet Disord. 2004 Feb 26;5:6. [Content Brief]

[3]. Shelly C Lu, et al. S-adenosylmethionine in liver health, injury, and cancer. Physiol Rev. 2012 Oct;92(4):1515-42. [Content Brief]

[4]. Mosca L, et al. Effects of S‑adenosyl‑L‑methionine on the invasion and migration of head and neck squamous cancer cells and analysis of the underlying mechanisms. Int J Oncol. 2020 May;56(5):1212-1224. [Content Brief]

[5]. Ham MS, et al. S-adenosyl methionine specifically protects the anticancer effect of 5-FU via DNMTs expression in human A549 lung cancer cells. Mol Clin Oncol. 2013 Mar;1(2):373-378. [Content Brief]

[6]. Ornoy A, et al. S-adenosyl methionine prevents ASD like behaviors triggered by early postnatal valproic acid exposure in very young mice. Neurotoxicol Teratol. 2019 Jan-Feb;71:64-74. [Content Brief]

[7]. Dhediya RM, et al. Evaluation of antiepileptic effect of S-adenosyl methionine and its role in memory impairment in pentylenetetrazole-induced kindling model in rats. Epilepsy Behav. 2016 Aug;61:153-157. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	1.7524 mL	8.7621 mL	17.5242 mL	43.8105 mL
	5 mM	0.3505 mL	1.7524 mL	3.5048 mL	8.7621 mL
	10 mM	0.1752 mL	0.8762 mL	1.7524 mL	4.3810 mL
	15 mM	0.1168 mL	0.5841 mL	1.1683 mL	2.9207 mL
	20 mM	0.0876 mL	0.4381 mL	0.8762 mL	2.1905 mL
	25 mM	0.0701 mL	0.3505 mL	0.7010 mL	1.7524 mL
	30 mM	0.0584 mL	0.2921 mL	0.5841 mL	1.4603 mL
	40 mM	0.0438 mL	0.2191 mL	0.4381 mL	1.0953 mL
	50 mM	0.0350 mL	0.1752 mL	0.3505 mL	0.8762 mL
	60 mM	0.0292 mL	0.1460 mL	0.2921 mL	0.7302 mL
	80 mM	0.0219 mL	0.1095 mL	0.2191 mL	0.5476 mL
	100 mM	0.0175 mL	0.0876 mL	0.1752 mL	0.4381 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Bioz

Keywords

S-Adenosyl-L-methionine tosylate

22 Cited Publications

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S-Adenosyl-L-methionine tosylate

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