5'-Methylthioadenosine
Based on 10 publication(s) in Google Scholar
5'-Methylthioadenosine (5'-(Methylthio)-5'-deoxyadenosine) is a nucleoside generated from S-adenosylmethionine (SAM) during polyamine synthesis. 5'-Methylthioadenosine suppresses tumors by inhibiting tumor cell proliferation, invasion, and the induction of apoptosis while controlling the inflammatory micro-environments of tumor tissue. 5'-Methylthioadenosine and its associated materials have striking regulatory effects on tumorigenesis.
For research use only. We do not sell to patients.
- Purity: 99.75%
- CAS No.: 2457-80-9
- Formula: C11H15N5O3S
- Molecular Weight:297.33
-
Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Anti-Infection Compound Library
HY-L002
-
Apoptosis Compound Library
HY-L003
-
Metabolism/Protease Compound Library
HY-L012
-
Natural Product Library
HY-L021
-
Anti-Cancer Compound Library
HY-L025
-
Human Endogenous Metabolite Compound Library
HY-L030
-
Medicine Food Homology Compound Library
HY-L055
-
Traditional Chinese Medicine Active Compound Library
HY-L065
-
Gut Microbial Metabolite Library
HY-L078
-
Antiparasitic Compound library
HY-L082
-
Microbial Metabolite Library
HY-L084
-
Food-Sourced Compound Library
HY-L094
-
Anti-Cancer Natural Product Library
HY-L107
-
Human Metabolite Library
HY-L123
-
Animal-Sourced Natural Product Library
HY-L157
-
Cell Death Library
HY-L162
-
Multi-Target Compound Library
HY-L176
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Natural Product Library Plus
HY-L021P
-
Natural Product and Natural Product-Like Compound Library
HY-L021M
-
Bioactive Compound Library
HY-L001
-
Miao Ethnicity Medicine Compound Library
HY-L190
-
Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library
HY-L194
-
Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
-
RO5 Drug-like Natural Product Library
HY-L200
-
High-Throughput Bioactive Compound Library
HY-L205
-
High-Throughput Natural Product Library
HY-L206
-
Mass Spectrometry Human Endogenous Metabolite Library
HY-L207
-
Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library
HY-L209
-
Mass Spectrometry Human Metabolite Library
HY-L215
-
Mouse Metabolite Compound Library
HY-L217
-
Diarrhea-Related Traditional Chinese Medicine Active Compound Library
HY-L224
-
Amino Acid Metabolite Compound Library
HY-L227
-
Mongolian Medicine Compound Library
HY-L238
-
Fungal-Sourced Compound Library
HY-L253
-
Mass Spectrometry Natural Product Library
HY-L262
-
MCE Kinase Hinge Binder Fragment Library
HY-L907
Publications Citing Use of MedChemExpress (MCE) 5'-Methylthioadenosine
More- Cell. 2025 Oct 30;188(22):6151-6169.e24. [Abstract]
- Immunity. 2025 Feb 11;58(2):381-396.e9. [Abstract]
- Clin Transl Med. 2025 Aug;15(8):e70424. [Abstract]
- J Mol Cell Cardiol. 2023 Jan:174:88-100. [Abstract]
- J Chromatogr A. 2025 May 10:1755:466036. [Abstract]
- Environ Toxicol. 2020 Feb;35(2):277-291. [Abstract]
- bioRxiv. 2026 Jun 22.
- bioRxiv. 2026 Jun 9:2026.06.05.730515. [Abstract]
- SSRN. 2025 Jun 12.
- Research Square Preprint. 2022 Feb.
Customer Validation & Images
-
ELISA
-
RT-PCR
-
WB
-
RT-PCR
All Endogenous Metabolite Isoforms
MoreAll Parasite Isoforms
More
Biological Activity
|
Human Endogenous Metabolite |
Microbial Metabolite |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| CCRF-CEM | IC50 |
150 μM
Compound: MTA
|
The concentration required to inhibit cell growth by 50% was measured on human CCRF-CEM cells (96h after exposure)
The concentration required to inhibit cell growth by 50% was measured on human CCRF-CEM cells (96h after exposure)
|
[PMID: 1908523] |
| L1210 | IC50 |
600 μM
Compound: 3
|
Compound was evaluated for its ability to inhibit growth by 50% at 48 hr in L1210 murine leukemia cells
Compound was evaluated for its ability to inhibit growth by 50% at 48 hr in L1210 murine leukemia cells
|
[PMID: 2496231] |
| L1210 | IC50 |
850 μM
Compound: MTA
|
The concentration required to inhibit cell growth by 50% was measured on L1210 Leukemic cell line of mouse(48h after exposure)
The concentration required to inhibit cell growth by 50% was measured on L1210 Leukemic cell line of mouse(48h after exposure)
|
[PMID: 1908523] |
| L5178Y | IC50 |
200 μM
Compound: MTA
|
The concentration required to inhibit cell growth by 50% was measured on L5178Y Leukemic cell line of mouse(48h after exposure)
The concentration required to inhibit cell growth by 50% was measured on L5178Y Leukemic cell line of mouse(48h after exposure)
|
[PMID: 1908523] |
| L5178Y | IC50 |
60 μM
Compound: 3
|
Compound was evaluated for its ability to inhibit growth by 50% at 48 hr in L5178Y murine leukemia cells
Compound was evaluated for its ability to inhibit growth by 50% at 48 hr in L5178Y murine leukemia cells
|
[PMID: 2496231] |
| MOLT-4 | IC50 |
11 μM
Compound: MTA
|
The concentration required to inhibit cell growth by 50% was measured on human MOLT-4 cells (96h after exposure)
The concentration required to inhibit cell growth by 50% was measured on human MOLT-4 cells (96h after exposure)
|
[PMID: 1908523] |
5'-Methylthioadenosine protects MTAP+ cells significantly better than MTAP cells from 6TG (6′-thioguanine) toxicity[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:MTAP+ and MTAP− NIH3T3 cells, isogenic MTAP+ and MTAP− cell line derived from a human HT1080 fibrosarcoma cell line
-
Concentration:6TG and 2FA in combination with 10 μM MTA
-
Incubation Time:48 hours
-
Result:Significantly increased IC50 concentration in MTAP+ cells, but no significant change in MTAP− cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 2457-80-9
-
Appearance Solid
-
Molecular Weight 297.33
-
Formula C11H15N5O3S
-
Color Off-white to light yellow
-
SMILES
NC1=NC=NC2=C1N=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](CSC)O3
-
Synonyms
5'-(Methylthio)-5'-deoxyadenosine; 5'-Deoxy-5'-(methylthio)adenosine; 5'-S-Methyl-5'-thioadenosine
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (10)
-
Journal Impact Factor
-
Most Recent
-
Cell
Adenosine kinase and ADAL coordinate detoxification of modified adenosines to safeguard metabolism. [Abstract]2025 Oct 30;188(22):6151-6169.e24. PMID: 40840445 -
Immunity
Microbiota translocation following intestinal barrier disruption promotes Mincle-mediated training of myeloid progenitors in the bone marrow. [Abstract]2025 Feb 11;58(2):381-396.e9. PMID: 39848243
5'-Methylthioadenosine purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Feb 11;58(2):381-396.e9. [Abstract]
BMDMs were treated or not with 5'-Methylthioadenosine (MTA, 500 μM) 1 h before plating in trehalose-6,6-dibehenate (TDB)-coated wells. After 24 h, cells were re-plated in non-TDB-coated plates and rested for 5 days before stimulation with CpG for 24 h. TNF (left) and IL-6 (right) production measured by ELISA in culture supernatant of BMDMs from indicated treatments and stimulations.
-
Clin Transl Med
Analysis of hepatitis B virus integration identifies KMT2B as a novel cancer-related gene in pancreatic cancer. [Abstract]2025 Aug;15(8):e70424. PMID: 40741875 -
J Mol Cell Cardiol
Adenosine kinase promotes post-infarction cardiac repair by epigenetically maintaining reparative macrophage phenotype. [Abstract]2023 Jan:174:88-100. PMID: 36473288
5'-Methylthioadenosine purchased from MedChemExpress. Usage Cited in: J Mol Cell Cardiol. 2023 Jan:174:88-100. [Abstract]
qPCR analysis of the mRNA level of Irf4 in BMDMs pretreated with medium or 5'-Methylthioadenosine (MTA, 1 mM) after IL-4 (10 ng/mL) treatment for 6 h. n = 3–4 per group.
5'-Methylthioadenosine purchased from MedChemExpress. Usage Cited in: J Mol Cell Cardiol. 2023 Jan:174:88-100. [Abstract]
Immunoblot analysis of IRF4 and H3K4me3 levels in BMDMs pretreated with medium or 5'-Methylthioadenosine (MTA, 1 mM) after IL-4 (10 ng/mL) treatment for 24 h.
-
J Chromatogr A
Quantification of five intracellular and extracellular methionine pathway intermediates using stable isotope dilution UHPLC-MS/MS. [Abstract]2025 May 10:1755:466036. PMID: 40398135 -
Environ Toxicol
Epigenetic modification of H3K4 and oxidative stress are involved in MC-LR-induced apoptosis in testicular cells of SD rats. [Abstract]2020 Feb;35(2):277-291. PMID: 31691492
5'-Methylthioadenosine purchased from MedChemExpress. Usage Cited in: Environ Toxicol. 2020 Feb;35(2):277-291. [Abstract]
The expression of H3K4me3 and apoptosis-related genes in cocultured SD rat Sertoli-germ cells after exposed to 0, 9, 18, 36 (μg/mL) MC-LR or MC-LR (36 μg/mL) + 5'-Methylthioadenosine (MTA, 1 mmoL/L). The mRNA levels of apoptosis-related genes in the apoptosis signaling pathway.
-
-
bioRxiv
Revealing the spatiotemporal dynamics of methionine metabolism with a genetically encoded single-fluorophore biosensor. [Abstract]2026 Jun 9:2026.06.05.730515. PMID: 42327153 -
-
Solvent & Solubility
DMSO : 100 mg/mL (336.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.41 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.41 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (279 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
-
Handling Instructions (2659 KB)
References
[1]. Yaofeng Li, et al. 5'-Methylthioadenosine and Cancer: old molecules, new understanding. J Cancer. 2019;10(4):927-936. [Content Brief]
[2]. Tang Y, et al. 5'-Methylthioadenosine attenuates ischemia reperfusion injury after liver transplantation in rats. Inflammation. 2014;37(5):1366-1373. [Content Brief]
[4]. Tang B, et al. Specific Targeting of MTAP-Deleted Tumors with a Combination of 2'-Fluoroadenine and 5'-Methylthioadenosine. Cancer Res. 2018;78(15):4386-4395. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3633 mL | 16.8163 mL | 33.6327 mL | 84.0817 mL |
| 5 mM | 0.6727 mL | 3.3633 mL | 6.7265 mL | 16.8163 mL | |
| 10 mM | 0.3363 mL | 1.6816 mL | 3.3633 mL | 8.4082 mL | |
| 15 mM | 0.2242 mL | 1.1211 mL | 2.2422 mL | 5.6054 mL | |
| 20 mM | 0.1682 mL | 0.8408 mL | 1.6816 mL | 4.2041 mL | |
| 25 mM | 0.1345 mL | 0.6727 mL | 1.3453 mL | 3.3633 mL | |
| 30 mM | 0.1121 mL | 0.5605 mL | 1.1211 mL | 2.8027 mL | |
| 40 mM | 0.0841 mL | 0.4204 mL | 0.8408 mL | 2.1020 mL | |
| 50 mM | 0.0673 mL | 0.3363 mL | 0.6727 mL | 1.6816 mL | |
| 60 mM | 0.0561 mL | 0.2803 mL | 0.5605 mL | 1.4014 mL | |
| 80 mM | 0.0420 mL | 0.2102 mL | 0.4204 mL | 1.0510 mL | |
| 100 mM | 0.0336 mL | 0.1682 mL | 0.3363 mL | 0.8408 mL |
Powered by Bioz