SAH  (Synonyms: SAH (S-Adenosylhomocysteine))

SAH (S-Adenosylhomocysteine) is an amino acid derivative and a modulartor in several metabolic pathways. It is an intermediate in the synthesis of cysteine and adenosine^[1]. SAH is an inhibitor for METTL3-METTL14 heterodimer complex (METTL3-14) with an IC₅₀ of 0.9 µM^[2].

SAH (10^-7-10^-5 M) activates norepinephrine (NE) and serotonin (5-HT) in vitro uptake in synaptosomal preparations from rat brain, but does not affect dopamine (DA) uptake^[1].
SAH (0.1-10 μM) shows strong inhibitory effects on METTL3-14 activity with an IC₅₀ value of 0.9 μM in Kinetic parameter determination of the METTL3-14 complex^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

SAH (administered i.v., i.p. or p.o. in 1-10 mg/kg) is sleep-inductive and anticonvulsant with rabbit, rat and cat^[1].
SAH (intraperitoneal injection; 7 mg/kg; 1 h prior to sacrifice) induces in vitro uptake increase for NE and 5-HT, significant only in brain stem and midbrain, but does not alter DA uptake^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

384.41

Solid

C₁₄H₂₀N₆O₅S

979-92-0

NC1=NC=NC2=C1N=CN2[C@@H]3O[C@H](CSCC[C@H](N)C(O)=O)[C@@H](O)[C@H]3O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. DE LA HABA G, et al. The enzymatic synthesis of S-adenosyl-L-homocysteine from adenosine and homocysteine. J Biol Chem. 1959 Mar;234(3):603-8.  [Content Brief]

  [2]. Li F, et al. A Radioactivity-Based Assay for Screening Human m6A-RNA Methyltransferase, METTL3-METTL14 Complex, and Demethylase ALKBH5. Biomol Screen. 2016 Mar;21(3):290-7.  [Content Brief]